%0 Journal Article
%A Wefers, Benedikt
%A Brandl, Christina
%A Ortiz, Oskar
%A Wurst, Wolfgang
%A Kühn, Ralf
%T Genome Editing in Mice Using TALE Nucleases.
%J Methods in molecular biology
%V 1338
%@ 1064-3745
%C [Heidelberg]
%I [Springer]
%M DZNE-2020-04656
%@ 978-1-4939-2931-3 (print)
%B Methods in Molecular Biology
%P 229-243
%D 2016
%< TALENs / Kühn, Ralf (Editor)   ; New York, NY : Springer New York, 2016, Chapter 17 ; ISSN: 1064-3745=1940-6029 ; ISBN: 978-1-4939-2931-3=978-1-4939-2932-0 ; doi:10.1007/978-1-4939-2932-0
%X Gene engineering for generating targeted mouse mutants is a key technology for biomedical research. Using TALENs as sequence-specific nucleases to induce targeted double-strand breaks, the mouse genome can be directly modified in zygotes in a single step without the need for embryonic stem cells. By embryo microinjection of TALEN mRNAs and targeting vectors, knockout and knock-in alleles can be generated fast and efficiently. In this chapter we provide protocols for the application of TALENs in mouse zygotes.
%K Animals
%K Animals, Genetically Modified: genetics
%K DNA Breaks, Double-Stranded
%K Endonucleases: genetics
%K Gene Knockout Techniques: methods
%K Genome
%K Mice
%K Microinjections
%K Mutation
%K RNA Editing: genetics
%K RNA, Messenger: genetics
%K RNA, Messenger (NLM Chemicals)
%K Endonucleases (NLM Chemicals)
%F PUB:(DE-HGF)3 ; PUB:(DE-HGF)16
%9 BookJournal Article
%$ pmid:26443225
%R 10.1007/978-1-4939-2932-0_17
%U https://pub.dzne.de/record/138334