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000138334 005__ 20250416111447.0
000138334 020__ $$a978-1-4939-2931-3 (print)
000138334 020__ $$a978-1-4939-2932-0 (electronic)
000138334 0247_ $$2doi$$a10.1007/978-1-4939-2932-0_17
000138334 0247_ $$2pmid$$apmid:26443225
000138334 0247_ $$2ISSN$$a1064-3745
000138334 0247_ $$2ISSN$$a1940-6029
000138334 0247_ $$2altmetric$$aaltmetric:4603489
000138334 037__ $$aDZNE-2020-04656
000138334 041__ $$aEnglish
000138334 082__ $$a570
000138334 1001_ $$0P:(DE-2719)2810988$$aWefers, Benedikt$$b0$$eFirst author$$udzne
000138334 245__ $$aGenome Editing in Mice Using TALE Nucleases.
000138334 260__ $$a[Heidelberg]$$b[Springer]$$c2016
000138334 264_1 $$2Crossref$$3print$$bSpringer New York$$c2016-01-01
000138334 29510 $$aTALENs / Kühn, Ralf (Editor)   ; New York, NY : Springer New York, 2016, Chapter 17 ; ISSN: 1064-3745=1940-6029 ; ISBN: 978-1-4939-2931-3=978-1-4939-2932-0 ; doi:10.1007/978-1-4939-2932-0
000138334 300__ $$a229 - 243
000138334 3367_ $$2DRIVER$$aarticle
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000138334 3367_ $$0PUB:(DE-HGF)16$$2PUB:(DE-HGF)$$aJournal Article$$bjournal$$mjournal$$s1744794791_2859
000138334 3367_ $$2BibTeX$$aARTICLE
000138334 3367_ $$2ORCID$$aJOURNAL_ARTICLE
000138334 3367_ $$00$$2EndNote$$aJournal Article
000138334 4900_ $$aMethods in Molecular Biology$$v1338
000138334 520__ $$aGene engineering for generating targeted mouse mutants is a key technology for biomedical research. Using TALENs as sequence-specific nucleases to induce targeted double-strand breaks, the mouse genome can be directly modified in zygotes in a single step without the need for embryonic stem cells. By embryo microinjection of TALEN mRNAs and targeting vectors, knockout and knock-in alleles can be generated fast and efficiently. In this chapter we provide protocols for the application of TALENs in mouse zygotes.
000138334 536__ $$0G:(DE-HGF)POF3-342$$a342 - Disease Mechanisms and Model Systems (POF3-342)$$cPOF3-342$$fPOF III$$x0
000138334 542__ $$2Crossref$$i2016-01-01$$uhttp://www.springer.com/tdm
000138334 588__ $$aDataset connected to CrossRef Book Series, PubMed,
000138334 650_7 $$2NLM Chemicals$$aRNA, Messenger
000138334 650_7 $$0EC 3.1.-$$2NLM Chemicals$$aEndonucleases
000138334 650_2 $$2MeSH$$aAnimals
000138334 650_2 $$2MeSH$$aAnimals, Genetically Modified: genetics
000138334 650_2 $$2MeSH$$aDNA Breaks, Double-Stranded
000138334 650_2 $$2MeSH$$aEndonucleases: genetics
000138334 650_2 $$2MeSH$$aGene Knockout Techniques: methods
000138334 650_2 $$2MeSH$$aGenome
000138334 650_2 $$2MeSH$$aMice
000138334 650_2 $$2MeSH$$aMicroinjections
000138334 650_2 $$2MeSH$$aMutation
000138334 650_2 $$2MeSH$$aRNA Editing: genetics
000138334 650_2 $$2MeSH$$aRNA, Messenger: genetics
000138334 7001_ $$aBrandl, Christina$$b1
000138334 7001_ $$aOrtiz, Oskar$$b2
000138334 7001_ $$0P:(DE-2719)2000028$$aWurst, Wolfgang$$b3$$udzne
000138334 7001_ $$aKühn, Ralf$$b4
000138334 77318 $$2Crossref$$3book-chapter$$a10.1007/978-1-4939-2932-0_17$$b : Springer New York, 2016-01-01$$p229-243$$tTALENs$$x1064-3745$$y2016
000138334 773__ $$0PERI:(DE-600)2493551-7$$a10.1007/978-1-4939-2932-0_17$$p229-243$$tMethods in molecular biology$$v1338$$x1064-3745$$y2016
000138334 8564_ $$uhttps://pub.dzne.de/record/138334/files/DZNE-2020-04656_Restricted.pdf
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000138334 909CO $$ooai:pub.dzne.de:138334$$pVDB
000138334 9101_ $$0I:(DE-588)1065079516$$6P:(DE-2719)2810988$$aDeutsches Zentrum für Neurodegenerative Erkrankungen$$b0$$kDZNE
000138334 9101_ $$0I:(DE-588)1065079516$$6P:(DE-2719)2000028$$aDeutsches Zentrum für Neurodegenerative Erkrankungen$$b3$$kDZNE
000138334 9131_ $$0G:(DE-HGF)POF3-342$$1G:(DE-HGF)POF3-340$$2G:(DE-HGF)POF3-300$$3G:(DE-HGF)POF3$$4G:(DE-HGF)POF$$aDE-HGF$$bGesundheit$$lErkrankungen des Nervensystems$$vDisease Mechanisms and Model Systems$$x0
000138334 9141_ $$y2016
000138334 915__ $$0StatID:(DE-HGF)0300$$2StatID$$aDBCoverage$$bMedline$$d2020-01-17
000138334 915__ $$0StatID:(DE-HGF)0310$$2StatID$$aDBCoverage$$bNCBI Molecular Biology Database$$d2020-01-17
000138334 915__ $$0StatID:(DE-HGF)0200$$2StatID$$aDBCoverage$$bSCOPUS$$d2020-01-17
000138334 9201_ $$0I:(DE-2719)1140001$$kAG Wurst$$lGenome Engineering$$x0
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