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@ARTICLE{Wefers:138334,
      author       = {Wefers, Benedikt and Brandl, Christina and Ortiz, Oskar and
                      Wurst, Wolfgang and Kühn, Ralf},
      title        = {{G}enome {E}diting in {M}ice {U}sing {TALE} {N}ucleases.},
      journal      = {Methods in molecular biology},
      volume       = {1338},
      issn         = {1064-3745},
      address      = {[Heidelberg]},
      publisher    = {[Springer]},
      reportid     = {DZNE-2020-04656},
      isbn         = {978-1-4939-2931-3 (print)},
      series       = {Methods in Molecular Biology},
      pages        = {229-243},
      year         = {2016},
      comment      = {TALENs / Kühn, Ralf (Editor) ; New York, NY : Springer New
                      York, 2016, Chapter 17 ; ISSN: 1064-3745=1940-6029 ; ISBN:
                      978-1-4939-2931-3=978-1-4939-2932-0 ;
                      doi:10.1007/978-1-4939-2932-0},
      booktitle     = {TALENs / Kühn, Ralf (Editor) ; New
                       York, NY : Springer New York, 2016,
                       Chapter 17 ; ISSN: 1064-3745=1940-6029
                       ; ISBN:
                       978-1-4939-2931-3=978-1-4939-2932-0 ;
                       doi:10.1007/978-1-4939-2932-0},
      abstract     = {Gene engineering for generating targeted mouse mutants is a
                      key technology for biomedical research. Using TALENs as
                      sequence-specific nucleases to induce targeted double-strand
                      breaks, the mouse genome can be directly modified in zygotes
                      in a single step without the need for embryonic stem cells.
                      By embryo microinjection of TALEN mRNAs and targeting
                      vectors, knockout and knock-in alleles can be generated fast
                      and efficiently. In this chapter we provide protocols for
                      the application of TALENs in mouse zygotes.},
      keywords     = {Animals / Animals, Genetically Modified: genetics / DNA
                      Breaks, Double-Stranded / Endonucleases: genetics / Gene
                      Knockout Techniques: methods / Genome / Mice /
                      Microinjections / Mutation / RNA Editing: genetics / RNA,
                      Messenger: genetics / RNA, Messenger (NLM Chemicals) /
                      Endonucleases (NLM Chemicals)},
      cin          = {AG Wurst},
      ddc          = {570},
      cid          = {I:(DE-2719)1140001},
      pnm          = {342 - Disease Mechanisms and Model Systems (POF3-342)},
      pid          = {G:(DE-HGF)POF3-342},
      typ          = {PUB:(DE-HGF)3 / PUB:(DE-HGF)16},
      pubmed       = {pmid:26443225},
      doi          = {10.1007/978-1-4939-2932-0_17},
      url          = {https://pub.dzne.de/record/138334},
}