%0 Journal Article
%A Paquet, Dominik
%A Plucińska, Gabriela
%A Misgeld, Thomas
%T In vivo imaging of mitochondria in intact zebrafish larvae.
%J Methods in enzymology
%V 547
%@ 0076-6879
%C New York, NY [u.a.]
%I Elsevier
%M DZNE-2020-05038
%B Methods in Enzymology
%P 151-164
%D 2014
%< Mitochondrial Function / Paquet, Dominik  ;  : Elsevier, 2014,  ; ISSN: 00766879 ; ISBN: 9780128014158 ; doi:10.1016/B978-0-12-801415-8.00009-6
%X Visualizing neuronal mitochondria in a living, intact mammalian organism is a challenge that can be overcome in zebrafish larvae, which are highly accessible for optical imaging and genetic manipulation. Here, we detail an approach to visualize neuronal mitochondria in sensory Rohon-Beard axons, which allows quantitatively measuring mitochondrial shape, dynamics, and transport in vivo. This provides a useful assay for basic studies exploring the behavior of neuronal mitochondria in their natural habitat, for revealing the influence that disease-related alterations have on this behavior and for testing pharmacological compounds and genetic manipulations that might ameliorate disease-related mitochondrial phenotypes in neurons.
%K Animals
%K Animals, Genetically Modified
%K Axonal Transport
%K Axons
%K Bacterial Proteins: genetics
%K Bacterial Proteins: metabolism
%K Electronic Data Processing
%K Embryo, Nonmammalian: cytology
%K Image Processing, Computer-Assisted
%K Larva: cytology
%K Luminescent Proteins: genetics
%K Luminescent Proteins: metabolism
%K Microscopy, Fluorescence: instrumentation
%K Microscopy, Fluorescence: methods
%K Mitochondria: physiology
%K Sensory Receptor Cells: cytology
%K Zebrafish: embryology
%K Zebrafish: genetics
%K Bacterial Proteins (NLM Chemicals)
%K Luminescent Proteins (NLM Chemicals)
%K yellow fluorescent protein, Bacteria (NLM Chemicals)
%F PUB:(DE-HGF)3 ; PUB:(DE-HGF)16
%9 BookJournal Article
%$ pmid:25416357
%R 10.1016/B978-0-12-801415-8.00009-6
%U https://pub.dzne.de/record/138716