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000138716 0247_ $$2doi$$a10.1016/B978-0-12-801415-8.00009-6
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000138716 0247_ $$2ISSN$$a0076-6879
000138716 0247_ $$2ISSN$$a1079-2376
000138716 0247_ $$2ISSN$$a1557-7988
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000138716 037__ $$aDZNE-2020-05038
000138716 041__ $$aEnglish
000138716 082__ $$a570
000138716 1001_ $$0P:(DE-2719)2010112$$aPaquet, Dominik$$b0$$eFirst author$$udzne
000138716 245__ $$aIn vivo imaging of mitochondria in intact zebrafish larvae.
000138716 260__ $$aNew York, NY [u.a.]$$bElsevier$$c2014
000138716 264_1 $$2Crossref$$3print$$bElsevier$$c2014-01-01
000138716 29510 $$aMitochondrial Function / Paquet, Dominik  ;  : Elsevier, 2014,  ; ISSN: 00766879 ; ISBN: 9780128014158 ; doi:10.1016/B978-0-12-801415-8.00009-6
000138716 300__ $$a151 - 164
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000138716 4900_ $$aMethods in Enzymology$$v547
000138716 520__ $$aVisualizing neuronal mitochondria in a living, intact mammalian organism is a challenge that can be overcome in zebrafish larvae, which are highly accessible for optical imaging and genetic manipulation. Here, we detail an approach to visualize neuronal mitochondria in sensory Rohon-Beard axons, which allows quantitatively measuring mitochondrial shape, dynamics, and transport in vivo. This provides a useful assay for basic studies exploring the behavior of neuronal mitochondria in their natural habitat, for revealing the influence that disease-related alterations have on this behavior and for testing pharmacological compounds and genetic manipulations that might ameliorate disease-related mitochondrial phenotypes in neurons.
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000138716 650_7 $$2NLM Chemicals$$aBacterial Proteins
000138716 650_7 $$2NLM Chemicals$$aLuminescent Proteins
000138716 650_7 $$2NLM Chemicals$$ayellow fluorescent protein, Bacteria
000138716 650_2 $$2MeSH$$aAnimals
000138716 650_2 $$2MeSH$$aAnimals, Genetically Modified
000138716 650_2 $$2MeSH$$aAxonal Transport
000138716 650_2 $$2MeSH$$aAxons
000138716 650_2 $$2MeSH$$aBacterial Proteins: genetics
000138716 650_2 $$2MeSH$$aBacterial Proteins: metabolism
000138716 650_2 $$2MeSH$$aElectronic Data Processing
000138716 650_2 $$2MeSH$$aEmbryo, Nonmammalian: cytology
000138716 650_2 $$2MeSH$$aImage Processing, Computer-Assisted
000138716 650_2 $$2MeSH$$aLarva: cytology
000138716 650_2 $$2MeSH$$aLuminescent Proteins: genetics
000138716 650_2 $$2MeSH$$aLuminescent Proteins: metabolism
000138716 650_2 $$2MeSH$$aMicroscopy, Fluorescence: instrumentation
000138716 650_2 $$2MeSH$$aMicroscopy, Fluorescence: methods
000138716 650_2 $$2MeSH$$aMitochondria: physiology
000138716 650_2 $$2MeSH$$aSensory Receptor Cells: cytology
000138716 650_2 $$2MeSH$$aZebrafish: embryology
000138716 650_2 $$2MeSH$$aZebrafish: genetics
000138716 7001_ $$0P:(DE-HGF)0$$aPlucińska, Gabriela$$b1
000138716 7001_ $$0P:(DE-2719)2810727$$aMisgeld, Thomas$$b2$$eLast author$$udzne
000138716 77318 $$2Crossref$$3book-chapter$$a10.1016/b978-0-12-801415-8.00009-6$$b : Elsevier, 2014-01-01$$p151-164$$tMethods in Enzymology$$x0076-6879$$y2014
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000138716 9141_ $$y2014
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