TY  - JOUR
AU  - Paquet, Dominik
AU  - Plucińska, Gabriela
AU  - Misgeld, Thomas
TI  - In vivo imaging of mitochondria in intact zebrafish larvae.
JO  - Methods in enzymology
VL  - 547
SN  - 0076-6879
CY  - New York, NY [u.a.]
PB  - Elsevier
M1  - DZNE-2020-05038
T2  - Methods in Enzymology
SP  - 151-164
PY  - 2014
AB  - Visualizing neuronal mitochondria in a living, intact mammalian organism is a challenge that can be overcome in zebrafish larvae, which are highly accessible for optical imaging and genetic manipulation. Here, we detail an approach to visualize neuronal mitochondria in sensory Rohon-Beard axons, which allows quantitatively measuring mitochondrial shape, dynamics, and transport in vivo. This provides a useful assay for basic studies exploring the behavior of neuronal mitochondria in their natural habitat, for revealing the influence that disease-related alterations have on this behavior and for testing pharmacological compounds and genetic manipulations that might ameliorate disease-related mitochondrial phenotypes in neurons.
KW  - Animals
KW  - Animals, Genetically Modified
KW  - Axonal Transport
KW  - Axons
KW  - Bacterial Proteins: genetics
KW  - Bacterial Proteins: metabolism
KW  - Electronic Data Processing
KW  - Embryo, Nonmammalian: cytology
KW  - Image Processing, Computer-Assisted
KW  - Larva: cytology
KW  - Luminescent Proteins: genetics
KW  - Luminescent Proteins: metabolism
KW  - Microscopy, Fluorescence: instrumentation
KW  - Microscopy, Fluorescence: methods
KW  - Mitochondria: physiology
KW  - Sensory Receptor Cells: cytology
KW  - Zebrafish: embryology
KW  - Zebrafish: genetics
KW  - Bacterial Proteins (NLM Chemicals)
KW  - Luminescent Proteins (NLM Chemicals)
KW  - yellow fluorescent protein, Bacteria (NLM Chemicals)
LB  - PUB:(DE-HGF)3 ; PUB:(DE-HGF)16
C6  - pmid:25416357
DO  - DOI:10.1016/B978-0-12-801415-8.00009-6
UR  - https://pub.dzne.de/record/138716
ER  -