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@ARTICLE{Paquet:138716,
author = {Paquet, Dominik and Plucińska, Gabriela and Misgeld,
Thomas},
title = {{I}n vivo imaging of mitochondria in intact zebrafish
larvae.},
journal = {Methods in enzymology},
volume = {547},
issn = {0076-6879},
address = {New York, NY [u.a.]},
publisher = {Elsevier},
reportid = {DZNE-2020-05038},
series = {Methods in Enzymology},
pages = {151-164},
year = {2014},
comment = {Mitochondrial Function / Paquet, Dominik ; : Elsevier,
2014, ; ISSN: 00766879 ; ISBN: 9780128014158 ;
doi:10.1016/B978-0-12-801415-8.00009-6},
booktitle = {Mitochondrial Function / Paquet,
Dominik ; : Elsevier, 2014, ; ISSN:
00766879 ; ISBN: 9780128014158 ;
doi:10.1016/B978-0-12-801415-8.00009-6},
abstract = {Visualizing neuronal mitochondria in a living, intact
mammalian organism is a challenge that can be overcome in
zebrafish larvae, which are highly accessible for optical
imaging and genetic manipulation. Here, we detail an
approach to visualize neuronal mitochondria in sensory
Rohon-Beard axons, which allows quantitatively measuring
mitochondrial shape, dynamics, and transport in vivo. This
provides a useful assay for basic studies exploring the
behavior of neuronal mitochondria in their natural habitat,
for revealing the influence that disease-related alterations
have on this behavior and for testing pharmacological
compounds and genetic manipulations that might ameliorate
disease-related mitochondrial phenotypes in neurons.},
keywords = {Animals / Animals, Genetically Modified / Axonal Transport
/ Axons / Bacterial Proteins: genetics / Bacterial Proteins:
metabolism / Electronic Data Processing / Embryo,
Nonmammalian: cytology / Image Processing, Computer-Assisted
/ Larva: cytology / Luminescent Proteins: genetics /
Luminescent Proteins: metabolism / Microscopy, Fluorescence:
instrumentation / Microscopy, Fluorescence: methods /
Mitochondria: physiology / Sensory Receptor Cells: cytology
/ Zebrafish: embryology / Zebrafish: genetics / Bacterial
Proteins (NLM Chemicals) / Luminescent Proteins (NLM
Chemicals) / yellow fluorescent protein, Bacteria (NLM
Chemicals)},
cin = {AG Schmid / AG Misgeld},
ddc = {570},
cid = {I:(DE-2719)1140002 / I:(DE-2719)1110000-4},
pnm = {341 - Molecular Signaling (POF3-341)},
pid = {G:(DE-HGF)POF3-341},
typ = {PUB:(DE-HGF)3 / PUB:(DE-HGF)16},
pubmed = {pmid:25416357},
doi = {10.1016/B978-0-12-801415-8.00009-6},
url = {https://pub.dzne.de/record/138716},
}