%0 Journal Article
%A Manière, Gérard
%A Ziegler, Anna B
%A Geillon, Flore
%A Featherstone, David E
%A Grosjean, Yael
%T Direct Sensing of Nutrients via a LAT1-like Transporter in Drosophila Insulin-Producing Cells.
%J Cell reports
%V 17
%N 1
%@ 2211-1247
%C [New York, NY]
%I Elsevier
%M DZNE-2020-05117
%P 137-148
%D 2016
%X Dietary leucine has been suspected to play an important role in insulin release, a hormone that controls satiety and metabolism. The mechanism by which insulin-producing cells (IPCs) sense leucine and regulate insulin secretion is still poorly understood. In Drosophila, insulin-like peptides (DILP2 and DILP5) are produced by brain IPCs and are released in the hemolymph after leucine ingestion. Using Ca(2+)-imaging and ex vivo cultured larval brains, we demonstrate that IPCs can directly sense extracellular leucine levels via minidiscs (MND), a leucine transporter. MND knockdown in IPCs abolished leucine-dependent changes, including loss of DILP2 and DILP5 in IPC bodies, consistent with the idea that MND is necessary for leucine-dependent DILP release. This, in turn, leads to a strong increase in hemolymph sugar levels and reduced growth. GDH knockdown in IPCs also reduced leucine-dependent DILP release, suggesting that nutrient sensing is coupled to the glutamate dehydrogenase pathway.
%K Amino Acid Transport Systems: genetics
%K Amino Acid Transport Systems: metabolism
%K Animals
%K Brain: cytology
%K Brain: metabolism
%K Calcium: metabolism
%K Drosophila Proteins: genetics
%K Drosophila Proteins: metabolism
%K Drosophila melanogaster: cytology
%K Drosophila melanogaster: metabolism
%K Gene Expression Regulation
%K Glutamate Dehydrogenase: genetics
%K Glutamate Dehydrogenase: metabolism
%K Hemolymph: metabolism
%K Insulin-Secreting Cells: cytology
%K Insulin-Secreting Cells: metabolism
%K Insulins: genetics
%K Insulins: metabolism
%K Larva: cytology
%K Larva: metabolism
%K Leucine: administration & dosage
%K Leucine: metabolism
%K Protein Isoforms: genetics
%K Protein Isoforms: metabolism
%K Signal Transduction
%K Amino Acid Transport Systems (NLM Chemicals)
%K Drosophila Proteins (NLM Chemicals)
%K Ilp5 protein, Drosophila (NLM Chemicals)
%K Insulins (NLM Chemicals)
%K Mnd protein, Drosophila (NLM Chemicals)
%K Protein Isoforms (NLM Chemicals)
%K Glutamate Dehydrogenase (NLM Chemicals)
%K Leucine (NLM Chemicals)
%K Calcium (NLM Chemicals)
%F PUB:(DE-HGF)16
%9 Journal Article
%$ pmid:27681427
%2 pmc:PMC5055474
%R 10.1016/j.celrep.2016.08.093
%U https://pub.dzne.de/record/138795