%0 Journal Article
%A Pan, Chenchen
%A Cai, Ruiyao
%A Quacquarelli, Francesca Paola
%A Ghasemigharagoz, Alireza
%A Lourbopoulos, Athanasios
%A Matryba, Paweł
%A Plesnila, Nikolaus
%A Dichgans, Martin
%A Hellal, Farida
%A Ertürk, Ali
%T Shrinkage-mediated imaging of entire organs and organisms using uDISCO.
%J Nature methods
%V 13
%N 10
%@ 1548-7091
%C London [u.a.]
%I Nature Publishing Group
%M DZNE-2020-05139
%P 859-867
%D 2016
%X Recent tissue-clearing approaches have become important alternatives to standard histology approaches. However, light scattering in thick tissues and the size restrictions on samples that can be imaged with standard light-sheet microscopy pose limitations for analyzing large samples such as an entire rodent body. We developed 'ultimate DISCO' (uDISCO) clearing to overcome these limitations in volumetric imaging. uDISCO preserves fluorescent proteins over months and renders intact organs and rodent bodies transparent while reducing their size up to 65
%K Animals
%K Central Nervous System: blood supply
%K Central Nervous System: cytology
%K Contrast Media
%K Female
%K Green Fluorescent Proteins: analysis
%K Green Fluorescent Proteins: chemistry
%K Green Fluorescent Proteins: genetics
%K Half-Life
%K Humans
%K Imaging, Three-Dimensional: methods
%K Immunohistochemistry: methods
%K Male
%K Mice, Inbred C57BL
%K Mice, Transgenic
%K Microscopy, Fluorescence: methods
%K Neuroimaging: methods
%K Organ Specificity
%K Phenyl Ethers: chemistry
%K Rats
%K Single-Cell Analysis: methods
%K Solvents: chemistry
%K Staining and Labeling
%K Whole Body Imaging: methods
%K Contrast Media (NLM Chemicals)
%K Phenyl Ethers (NLM Chemicals)
%K Solvents (NLM Chemicals)
%K Green Fluorescent Proteins (NLM Chemicals)
%K phenyl ether (NLM Chemicals)
%F PUB:(DE-HGF)16
%9 Journal Article
%$ pmid:27548807
%R 10.1038/nmeth.3964
%U https://pub.dzne.de/record/138817