TY  - JOUR
AU  - Pan, Chenchen
AU  - Cai, Ruiyao
AU  - Quacquarelli, Francesca Paola
AU  - Ghasemigharagoz, Alireza
AU  - Lourbopoulos, Athanasios
AU  - Matryba, Paweł
AU  - Plesnila, Nikolaus
AU  - Dichgans, Martin
AU  - Hellal, Farida
AU  - Ertürk, Ali
TI  - Shrinkage-mediated imaging of entire organs and organisms using uDISCO.
JO  - Nature methods
VL  - 13
IS  - 10
SN  - 1548-7091
CY  - London [u.a.]
PB  - Nature Publishing Group
M1  - DZNE-2020-05139
SP  - 859-867
PY  - 2016
AB  - Recent tissue-clearing approaches have become important alternatives to standard histology approaches. However, light scattering in thick tissues and the size restrictions on samples that can be imaged with standard light-sheet microscopy pose limitations for analyzing large samples such as an entire rodent body. We developed 'ultimate DISCO' (uDISCO) clearing to overcome these limitations in volumetric imaging. uDISCO preserves fluorescent proteins over months and renders intact organs and rodent bodies transparent while reducing their size up to 65
KW  - Animals
KW  - Central Nervous System: blood supply
KW  - Central Nervous System: cytology
KW  - Contrast Media
KW  - Female
KW  - Green Fluorescent Proteins: analysis
KW  - Green Fluorescent Proteins: chemistry
KW  - Green Fluorescent Proteins: genetics
KW  - Half-Life
KW  - Humans
KW  - Imaging, Three-Dimensional: methods
KW  - Immunohistochemistry: methods
KW  - Male
KW  - Mice, Inbred C57BL
KW  - Mice, Transgenic
KW  - Microscopy, Fluorescence: methods
KW  - Neuroimaging: methods
KW  - Organ Specificity
KW  - Phenyl Ethers: chemistry
KW  - Rats
KW  - Single-Cell Analysis: methods
KW  - Solvents: chemistry
KW  - Staining and Labeling
KW  - Whole Body Imaging: methods
KW  - Contrast Media (NLM Chemicals)
KW  - Phenyl Ethers (NLM Chemicals)
KW  - Solvents (NLM Chemicals)
KW  - Green Fluorescent Proteins (NLM Chemicals)
KW  - phenyl ether (NLM Chemicals)
LB  - PUB:(DE-HGF)16
C6  - pmid:27548807
DO  - DOI:10.1038/nmeth.3964
UR  - https://pub.dzne.de/record/138817
ER  -