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@ARTICLE{Pan:138817,
      author       = {Pan, Chenchen and Cai, Ruiyao and Quacquarelli, Francesca
                      Paola and Ghasemigharagoz, Alireza and Lourbopoulos,
                      Athanasios and Matryba, Paweł and Plesnila, Nikolaus and
                      Dichgans, Martin and Hellal, Farida and Ertürk, Ali},
      title        = {{S}hrinkage-mediated imaging of entire organs and organisms
                      using u{DISCO}.},
      journal      = {Nature methods},
      volume       = {13},
      number       = {10},
      issn         = {1548-7091},
      address      = {London [u.a.]},
      publisher    = {Nature Publishing Group},
      reportid     = {DZNE-2020-05139},
      pages        = {859-867},
      year         = {2016},
      abstract     = {Recent tissue-clearing approaches have become important
                      alternatives to standard histology approaches. However,
                      light scattering in thick tissues and the size restrictions
                      on samples that can be imaged with standard light-sheet
                      microscopy pose limitations for analyzing large samples such
                      as an entire rodent body. We developed 'ultimate DISCO'
                      (uDISCO) clearing to overcome these limitations in
                      volumetric imaging. uDISCO preserves fluorescent proteins
                      over months and renders intact organs and rodent bodies
                      transparent while reducing their size up to $65\%.$ We used
                      uDISCO to image neuronal connections and vasculature from
                      head to toe over 7 cm and to perform unbiased screening of
                      transplanted stem cells within the entire body of adult
                      mice. uDISCO is compatible with diverse labeling methods and
                      archival human tissue, and it can readily be used in various
                      biomedical applications to study organization of large organ
                      systems throughout entire organisms.},
      keywords     = {Animals / Central Nervous System: blood supply / Central
                      Nervous System: cytology / Contrast Media / Female / Green
                      Fluorescent Proteins: analysis / Green Fluorescent Proteins:
                      chemistry / Green Fluorescent Proteins: genetics / Half-Life
                      / Humans / Imaging, Three-Dimensional: methods /
                      Immunohistochemistry: methods / Male / Mice, Inbred C57BL /
                      Mice, Transgenic / Microscopy, Fluorescence: methods /
                      Neuroimaging: methods / Organ Specificity / Phenyl Ethers:
                      chemistry / Rats / Single-Cell Analysis: methods / Solvents:
                      chemistry / Staining and Labeling / Whole Body Imaging:
                      methods / Contrast Media (NLM Chemicals) / Phenyl Ethers
                      (NLM Chemicals) / Solvents (NLM Chemicals) / Green
                      Fluorescent Proteins (NLM Chemicals) / phenyl ether (NLM
                      Chemicals)},
      cin          = {Clinical Dementia Research München},
      ddc          = {610},
      cid          = {I:(DE-2719)1111016},
      pnm          = {344 - Clinical and Health Care Research (POF3-344)},
      pid          = {G:(DE-HGF)POF3-344},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:27548807},
      doi          = {10.1038/nmeth.3964},
      url          = {https://pub.dzne.de/record/138817},
}