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| 001 | 138817 | ||
| 005 | 20240321220513.0 | ||
| 024 | 7 | _ | |a 10.1038/nmeth.3964 |2 doi |
| 024 | 7 | _ | |a pmid:27548807 |2 pmid |
| 024 | 7 | _ | |a 1548-7091 |2 ISSN |
| 024 | 7 | _ | |a 1548-7105 |2 ISSN |
| 024 | 7 | _ | |a altmetric:10759133 |2 altmetric |
| 037 | _ | _ | |a DZNE-2020-05139 |
| 041 | _ | _ | |a English |
| 082 | _ | _ | |a 610 |
| 100 | 1 | _ | |a Pan, Chenchen |0 P:(DE-HGF)0 |b 0 |
| 245 | _ | _ | |a Shrinkage-mediated imaging of entire organs and organisms using uDISCO. |
| 260 | _ | _ | |a London [u.a.] |c 2016 |b Nature Publishing Group |
| 264 | _ | 1 | |3 online |2 Crossref |b Springer Science and Business Media LLC |c 2016-08-22 |
| 264 | _ | 1 | |3 print |2 Crossref |b Springer Science and Business Media LLC |c 2016-10-01 |
| 336 | 7 | _ | |a article |2 DRIVER |
| 336 | 7 | _ | |a Output Types/Journal article |2 DataCite |
| 336 | 7 | _ | |a Journal Article |b journal |m journal |0 PUB:(DE-HGF)16 |s 1585579061_26570 |2 PUB:(DE-HGF) |
| 336 | 7 | _ | |a ARTICLE |2 BibTeX |
| 336 | 7 | _ | |a JOURNAL_ARTICLE |2 ORCID |
| 336 | 7 | _ | |a Journal Article |0 0 |2 EndNote |
| 520 | _ | _ | |a Recent tissue-clearing approaches have become important alternatives to standard histology approaches. However, light scattering in thick tissues and the size restrictions on samples that can be imaged with standard light-sheet microscopy pose limitations for analyzing large samples such as an entire rodent body. We developed 'ultimate DISCO' (uDISCO) clearing to overcome these limitations in volumetric imaging. uDISCO preserves fluorescent proteins over months and renders intact organs and rodent bodies transparent while reducing their size up to 65%. We used uDISCO to image neuronal connections and vasculature from head to toe over 7 cm and to perform unbiased screening of transplanted stem cells within the entire body of adult mice. uDISCO is compatible with diverse labeling methods and archival human tissue, and it can readily be used in various biomedical applications to study organization of large organ systems throughout entire organisms. |
| 536 | _ | _ | |a 344 - Clinical and Health Care Research (POF3-344) |0 G:(DE-HGF)POF3-344 |c POF3-344 |f POF III |x 0 |
| 542 | _ | _ | |i 2016-08-22 |2 Crossref |u http://www.springer.com/tdm |
| 588 | _ | _ | |a Dataset connected to CrossRef, PubMed, |
| 650 | _ | 7 | |a Contrast Media |2 NLM Chemicals |
| 650 | _ | 7 | |a Phenyl Ethers |2 NLM Chemicals |
| 650 | _ | 7 | |a Solvents |2 NLM Chemicals |
| 650 | _ | 7 | |a Green Fluorescent Proteins |0 147336-22-9 |2 NLM Chemicals |
| 650 | _ | 7 | |a phenyl ether |0 3O695R5M1U |2 NLM Chemicals |
| 650 | _ | 2 | |a Animals |2 MeSH |
| 650 | _ | 2 | |a Central Nervous System: blood supply |2 MeSH |
| 650 | _ | 2 | |a Central Nervous System: cytology |2 MeSH |
| 650 | _ | 2 | |a Contrast Media |2 MeSH |
| 650 | _ | 2 | |a Female |2 MeSH |
| 650 | _ | 2 | |a Green Fluorescent Proteins: analysis |2 MeSH |
| 650 | _ | 2 | |a Green Fluorescent Proteins: chemistry |2 MeSH |
| 650 | _ | 2 | |a Green Fluorescent Proteins: genetics |2 MeSH |
| 650 | _ | 2 | |a Half-Life |2 MeSH |
| 650 | _ | 2 | |a Humans |2 MeSH |
| 650 | _ | 2 | |a Imaging, Three-Dimensional: methods |2 MeSH |
| 650 | _ | 2 | |a Immunohistochemistry: methods |2 MeSH |
| 650 | _ | 2 | |a Male |2 MeSH |
| 650 | _ | 2 | |a Mice, Inbred C57BL |2 MeSH |
| 650 | _ | 2 | |a Mice, Transgenic |2 MeSH |
| 650 | _ | 2 | |a Microscopy, Fluorescence: methods |2 MeSH |
| 650 | _ | 2 | |a Neuroimaging: methods |2 MeSH |
| 650 | _ | 2 | |a Organ Specificity |2 MeSH |
| 650 | _ | 2 | |a Phenyl Ethers: chemistry |2 MeSH |
| 650 | _ | 2 | |a Rats |2 MeSH |
| 650 | _ | 2 | |a Single-Cell Analysis: methods |2 MeSH |
| 650 | _ | 2 | |a Solvents: chemistry |2 MeSH |
| 650 | _ | 2 | |a Staining and Labeling |2 MeSH |
| 650 | _ | 2 | |a Whole Body Imaging: methods |2 MeSH |
| 700 | 1 | _ | |a Cai, Ruiyao |0 P:(DE-HGF)0 |b 1 |
| 700 | 1 | _ | |a Quacquarelli, Francesca Paola |0 P:(DE-HGF)0 |b 2 |
| 700 | 1 | _ | |a Ghasemigharagoz, Alireza |0 P:(DE-HGF)0 |b 3 |
| 700 | 1 | _ | |a Lourbopoulos, Athanasios |0 P:(DE-HGF)0 |b 4 |
| 700 | 1 | _ | |a Matryba, Paweł |0 P:(DE-HGF)0 |b 5 |
| 700 | 1 | _ | |a Plesnila, Nikolaus |0 P:(DE-HGF)0 |b 6 |
| 700 | 1 | _ | |a Dichgans, Martin |0 P:(DE-2719)2000030 |b 7 |u dzne |
| 700 | 1 | _ | |a Hellal, Farida |0 P:(DE-HGF)0 |b 8 |
| 700 | 1 | _ | |a Ertürk, Ali |0 P:(DE-HGF)0 |b 9 |e Corresponding author |
| 773 | 1 | 8 | |a 10.1038/nmeth.3964 |b : Springer Science and Business Media LLC, 2016-08-22 |n 10 |p 859-867 |3 journal-article |2 Crossref |t Nature Methods |v 13 |y 2016 |x 1548-7091 |
| 773 | _ | _ | |a 10.1038/nmeth.3964 |g Vol. 13, no. 10, p. 859 - 867 |0 PERI:(DE-600)2163081-1 |n 10 |q 13:10<859 - 867 |p 859-867 |t Nature methods |v 13 |y 2016 |x 1548-7091 |
| 909 | C | O | |o oai:pub.dzne.de:138817 |p VDB |
| 910 | 1 | _ | |a Deutsches Zentrum für Neurodegenerative Erkrankungen |0 I:(DE-588)1065079516 |k DZNE |b 7 |6 P:(DE-2719)2000030 |
| 913 | 1 | _ | |a DE-HGF |b Forschungsbereich Gesundheit |l Erkrankungen des Nervensystems |1 G:(DE-HGF)POF3-340 |0 G:(DE-HGF)POF3-344 |2 G:(DE-HGF)POF3-300 |v Clinical and Health Care Research |x 0 |
| 914 | 1 | _ | |y 2016 |
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| 915 | _ | _ | |a JCR |0 StatID:(DE-HGF)0100 |2 StatID |b NAT METHODS : 2017 |
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| 920 | 1 | _ | |0 I:(DE-2719)1111016 |k Clinical Dementia Research München |l Clinical Dementia Research München |x 0 |
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| 980 | _ | _ | |a UNRESTRICTED |
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