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@ARTICLE{Krver:138902,
      author       = {Körver, Lisa and Melzer, Juliane and Aguado Roca, Eva and
                      Teichert, Dominic and Glatter, Timo and Arama, Eli and
                      Broemer, Meike},
      title        = {{T}he de-ubiquitylating enzyme {DUBA} is essential for
                      spermatogenesis in {D}rosophila.},
      journal      = {Cell death and differentiation},
      volume       = {23},
      number       = {12},
      issn         = {1350-9047},
      address      = {London},
      publisher    = {Macmillan},
      reportid     = {DZNE-2020-05224},
      pages        = {2019-2030},
      year         = {2016},
      abstract     = {De-ubiquitylating enzymes (DUBs) reverse protein
                      ubiquitylation and thereby control essential cellular
                      functions. Screening for a DUB that counteracts caspase
                      ubiquitylation to regulate cell survival, we identified the
                      Drosophila ovarian tumour-type DUB DUBA (CG6091). DUBA
                      physically interacts with the initiator caspase death
                      regulator Nedd2-like caspase (Dronc) and de-ubiquitylates
                      it, thereby contributing to efficient inhibitor of
                      apoptosis-antagonist-induced apoptosis in the fly eye.
                      Searching also for non-apoptotic functions of DUBA, we found
                      that Duba-null mutants are male sterile and display defects
                      in spermatid individualisation, a process that depends on
                      non-apoptotic caspase activity. Spermatids of DUBA-deficient
                      flies showed reduced caspase activity and lack critical
                      structures of the individualisation process. Biochemical
                      characterisation revealed an obligate activation step of
                      DUBA by phosphorylation. With genetic rescue experiments we
                      demonstrate that DUBA phosphorylation and catalytic activity
                      are crucial in vivo for DUBA function in spermatogenesis.
                      Our results demonstrate for the first time the importance of
                      de-ubiquitylation for fly spermatogenesis.},
      keywords     = {Amino Acid Sequence / Animals / Apoptosis / Biocatalysis /
                      Caspases: metabolism / Deubiquitinating Enzymes: chemistry /
                      Deubiquitinating Enzymes: metabolism / Drosophila Proteins:
                      chemistry / Drosophila Proteins: metabolism / Drosophila
                      melanogaster: enzymology / Drosophila melanogaster:
                      physiology / Male / Phosphorylation / Phosphoserine:
                      metabolism / Protein Binding / Spermatogenesis / Testis:
                      metabolism / Ubiquitinated Proteins: metabolism /
                      Ubiquitination / Drosophila Proteins (NLM Chemicals) /
                      Ubiquitinated Proteins (NLM Chemicals) / Phosphoserine (NLM
                      Chemicals) / DUBA protein, Drosophila (NLM Chemicals) /
                      Deubiquitinating Enzymes (NLM Chemicals) / Caspases (NLM
                      Chemicals)},
      cin          = {AG Brömer 2 / AG Brömer 1},
      ddc          = {610},
      cid          = {I:(DE-2719)1013021 / I:(DE-2719)5000021},
      pnm          = {341 - Molecular Signaling (POF3-341)},
      pid          = {G:(DE-HGF)POF3-341},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:27518434},
      pmc          = {pmc:PMC5136489},
      doi          = {10.1038/cdd.2016.79},
      url          = {https://pub.dzne.de/record/138902},
}