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| 001 | 138902 | ||
| 005 | 20240321220522.0 | ||
| 024 | 7 | _ | |a 10.1038/cdd.2016.79 |2 doi |
| 024 | 7 | _ | |a pmid:27518434 |2 pmid |
| 024 | 7 | _ | |a pmc:PMC5136489 |2 pmc |
| 024 | 7 | _ | |a 1350-9047 |2 ISSN |
| 024 | 7 | _ | |a 1476-5403 |2 ISSN |
| 024 | 7 | _ | |a altmetric:10474005 |2 altmetric |
| 037 | _ | _ | |a DZNE-2020-05224 |
| 041 | _ | _ | |a English |
| 082 | _ | _ | |a 610 |
| 100 | 1 | _ | |a Körver, Lisa |0 P:(DE-2719)2810874 |b 0 |e First author |u dzne |
| 245 | _ | _ | |a The de-ubiquitylating enzyme DUBA is essential for spermatogenesis in Drosophila. |
| 260 | _ | _ | |a London |c 2016 |b Macmillan |
| 264 | _ | 1 | |3 online |2 Crossref |b Springer Science and Business Media LLC |c 2016-08-12 |
| 264 | _ | 1 | |3 print |2 Crossref |b Springer Science and Business Media LLC |c 2016-12-01 |
| 336 | 7 | _ | |a article |2 DRIVER |
| 336 | 7 | _ | |a Output Types/Journal article |2 DataCite |
| 336 | 7 | _ | |a Journal Article |b journal |m journal |0 PUB:(DE-HGF)16 |s 1592980663_30012 |2 PUB:(DE-HGF) |
| 336 | 7 | _ | |a ARTICLE |2 BibTeX |
| 336 | 7 | _ | |a JOURNAL_ARTICLE |2 ORCID |
| 336 | 7 | _ | |a Journal Article |0 0 |2 EndNote |
| 520 | _ | _ | |a De-ubiquitylating enzymes (DUBs) reverse protein ubiquitylation and thereby control essential cellular functions. Screening for a DUB that counteracts caspase ubiquitylation to regulate cell survival, we identified the Drosophila ovarian tumour-type DUB DUBA (CG6091). DUBA physically interacts with the initiator caspase death regulator Nedd2-like caspase (Dronc) and de-ubiquitylates it, thereby contributing to efficient inhibitor of apoptosis-antagonist-induced apoptosis in the fly eye. Searching also for non-apoptotic functions of DUBA, we found that Duba-null mutants are male sterile and display defects in spermatid individualisation, a process that depends on non-apoptotic caspase activity. Spermatids of DUBA-deficient flies showed reduced caspase activity and lack critical structures of the individualisation process. Biochemical characterisation revealed an obligate activation step of DUBA by phosphorylation. With genetic rescue experiments we demonstrate that DUBA phosphorylation and catalytic activity are crucial in vivo for DUBA function in spermatogenesis. Our results demonstrate for the first time the importance of de-ubiquitylation for fly spermatogenesis. |
| 536 | _ | _ | |a 341 - Molecular Signaling (POF3-341) |0 G:(DE-HGF)POF3-341 |c POF3-341 |f POF III |x 0 |
| 542 | _ | _ | |i 2016-08-12 |2 Crossref |u http://www.springer.com/tdm |
| 588 | _ | _ | |a Dataset connected to CrossRef, PubMed, |
| 650 | _ | 7 | |a Drosophila Proteins |2 NLM Chemicals |
| 650 | _ | 7 | |a Ubiquitinated Proteins |2 NLM Chemicals |
| 650 | _ | 7 | |a Phosphoserine |0 17885-08-4 |2 NLM Chemicals |
| 650 | _ | 7 | |a DUBA protein, Drosophila |0 EC 3.4.19.12 |2 NLM Chemicals |
| 650 | _ | 7 | |a Deubiquitinating Enzymes |0 EC 3.4.19.12 |2 NLM Chemicals |
| 650 | _ | 7 | |a Caspases |0 EC 3.4.22.- |2 NLM Chemicals |
| 650 | _ | 2 | |a Amino Acid Sequence |2 MeSH |
| 650 | _ | 2 | |a Animals |2 MeSH |
| 650 | _ | 2 | |a Apoptosis |2 MeSH |
| 650 | _ | 2 | |a Biocatalysis |2 MeSH |
| 650 | _ | 2 | |a Caspases: metabolism |2 MeSH |
| 650 | _ | 2 | |a Deubiquitinating Enzymes: chemistry |2 MeSH |
| 650 | _ | 2 | |a Deubiquitinating Enzymes: metabolism |2 MeSH |
| 650 | _ | 2 | |a Drosophila Proteins: chemistry |2 MeSH |
| 650 | _ | 2 | |a Drosophila Proteins: metabolism |2 MeSH |
| 650 | _ | 2 | |a Drosophila melanogaster: enzymology |2 MeSH |
| 650 | _ | 2 | |a Drosophila melanogaster: physiology |2 MeSH |
| 650 | _ | 2 | |a Male |2 MeSH |
| 650 | _ | 2 | |a Phosphorylation |2 MeSH |
| 650 | _ | 2 | |a Phosphoserine: metabolism |2 MeSH |
| 650 | _ | 2 | |a Protein Binding |2 MeSH |
| 650 | _ | 2 | |a Spermatogenesis |2 MeSH |
| 650 | _ | 2 | |a Testis: metabolism |2 MeSH |
| 650 | _ | 2 | |a Ubiquitinated Proteins: metabolism |2 MeSH |
| 650 | _ | 2 | |a Ubiquitination |2 MeSH |
| 700 | 1 | _ | |a Melzer, Juliane |0 P:(DE-2719)2810805 |b 1 |u dzne |
| 700 | 1 | _ | |a Aguado Roca, Eva |0 P:(DE-2719)2810320 |b 2 |u dzne |
| 700 | 1 | _ | |a Teichert, Dominic |0 P:(DE-2719)2810393 |b 3 |u dzne |
| 700 | 1 | _ | |a Glatter, Timo |0 P:(DE-HGF)0 |b 4 |
| 700 | 1 | _ | |a Arama, Eli |0 P:(DE-HGF)0 |b 5 |
| 700 | 1 | _ | |a Broemer, Meike |0 P:(DE-2719)2810307 |b 6 |e Last author |u dzne |
| 773 | 1 | 8 | |a 10.1038/cdd.2016.79 |b : Springer Science and Business Media LLC, 2016-08-12 |n 12 |p 2019-2030 |3 journal-article |2 Crossref |t Cell Death & Differentiation |v 23 |y 2016 |x 1350-9047 |
| 773 | _ | _ | |a 10.1038/cdd.2016.79 |g Vol. 23, no. 12, p. 2019 - 2030 |0 PERI:(DE-600)1496681-5 |n 12 |q 23:12<2019 - 2030 |p 2019-2030 |t Cell death and differentiation |v 23 |y 2016 |x 1350-9047 |
| 856 | 7 | _ | |2 Pubmed Central |u http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5136489 |
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| 920 | 1 | _ | |0 I:(DE-2719)5000021 |k AG Brömer 1 |l Ubiquitin-mediated Processes in Neurodegenerative Diseases |x 1 |
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