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000138907 037__ $$aDZNE-2020-05229
000138907 041__ $$aEnglish
000138907 082__ $$a610
000138907 1001_ $$aJahnke, Heinz-Georg$$b0
000138907 245__ $$aA novel 384-multiwell microelectrode array for the impedimetric monitoring of Tau protein induced neurodegenerative processes.
000138907 260__ $$aAmsterdam [u.a.]$$bElsevier Science$$c2017
000138907 264_1 $$2Crossref$$3print$$bElsevier BV$$c2017-02-01
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000138907 520__ $$aOver the last decades, countless bioelectronic monitoring systems were developed for the analysis of cells as well as complex tissues. Most studies addressed the sensitivity and specificity of the bioelectronic detection method in comparison to classical molecular biological assays. In contrast, the up scaling as a prerequisite for the practical application of these novel bioelectronic monitoring systems is mostly only discussed theoretically. In this context, we developed a novel 384-multiwell microelectrode array (MMEA) based measurement system for the sensitive label-free real-time monitoring of neurodegenerative processes by impedance spectroscopy. With respect to the needs of productive screening systems for robust and reproducible measurements on high numbers of plates, we focused on reducing the critical contacting of more than 400 electrodes for a 384-MMEA. Therefore, we introduced an on top array of immersive counter electrodes that are individually addressed by a multiplexer and connected all measurement electrodes on the 384-MMEA to a single contact point. More strikingly, our novel approach provided a comparable signal stability and sensitivity similar to an array with integrated counter electrodes. Next, we optimized a SH-SY5Y cell based tauopathy model by introducing a novel 5-fold Tau mutation eliminating the need of artificial tauopathy induction. In combination with our novel 384-MMEA based measurement system, the concentration and time dependent neuroregenerative effect of the kinase inhibitor SRN-003-556 could be quantitatively monitored. Thus, our novel screening system could be a useful tool to identify and develop potential novel therapeutics in the field of Tau-related neurodegenerative diseases.
000138907 536__ $$0G:(DE-HGF)POF3-342$$a342 - Disease Mechanisms and Model Systems (POF3-342)$$cPOF3-342$$fPOF III$$x0
000138907 542__ $$2Crossref$$i2017-02-01$$uhttps://www.elsevier.com/tdm/userlicense/1.0/
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000138907 650_7 $$2NLM Chemicals$$aCarbazoles
000138907 650_7 $$2NLM Chemicals$$aSRN 003-556
000138907 650_7 $$2NLM Chemicals$$atau Proteins
000138907 650_2 $$2MeSH$$aCarbazoles: pharmacology
000138907 650_2 $$2MeSH$$aCell Line
000138907 650_2 $$2MeSH$$aDielectric Spectroscopy: instrumentation
000138907 650_2 $$2MeSH$$aDielectric Spectroscopy: methods
000138907 650_2 $$2MeSH$$aDrug Evaluation, Preclinical: instrumentation
000138907 650_2 $$2MeSH$$aDrug Evaluation, Preclinical: methods
000138907 650_2 $$2MeSH$$aEquipment Design
000138907 650_2 $$2MeSH$$aHumans
000138907 650_2 $$2MeSH$$aMicroelectrodes
000138907 650_2 $$2MeSH$$aTauopathies: diagnosis
000138907 650_2 $$2MeSH$$aTauopathies: drug therapy
000138907 650_2 $$2MeSH$$atau Proteins: analysis
000138907 7001_ $$aKrinke, Dana$$b1
000138907 7001_ $$aSeidel, Diana$$b2
000138907 7001_ $$aLilienthal, Katharina$$b3
000138907 7001_ $$0P:(DE-HGF)0$$aSchmidt, Sabine$$b4
000138907 7001_ $$aAzendorf, Ronny$$b5
000138907 7001_ $$aFischer, Michael$$b6
000138907 7001_ $$0P:(DE-2719)9000424$$aMack, Till$$b7$$udzne
000138907 7001_ $$0P:(DE-2719)9000420$$aStriggow, Frank$$b8$$udzne
000138907 7001_ $$aAlthaus, Holger$$b9
000138907 7001_ $$aSchober, Andreas$$b10
000138907 7001_ $$0P:(DE-HGF)0$$aRobitzki, Andrea A$$b11$$eCorresponding author
000138907 77318 $$2Crossref$$3journal-article$$a10.1016/j.bios.2016.07.074$$b : Elsevier BV, 2017-02-01$$p78-84$$tBiosensors and Bioelectronics$$v88$$x0956-5663$$y2017
000138907 773__ $$0PERI:(DE-600)1496379-6$$a10.1016/j.bios.2016.07.074$$gVol. 88, p. 78 - 84$$p78-84$$q88<78 - 84$$tBiosensors and bioelectronics$$v88$$x0956-5663$$y2017
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000138907 9141_ $$y2017
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