TY  - JOUR
AU  - Jahnke, Heinz-Georg
AU  - Krinke, Dana
AU  - Seidel, Diana
AU  - Lilienthal, Katharina
AU  - Schmidt, Sabine
AU  - Azendorf, Ronny
AU  - Fischer, Michael
AU  - Mack, Till
AU  - Striggow, Frank
AU  - Althaus, Holger
AU  - Schober, Andreas
AU  - Robitzki, Andrea A
TI  - A novel 384-multiwell microelectrode array for the impedimetric monitoring of Tau protein induced neurodegenerative processes.
JO  - Biosensors and bioelectronics
VL  - 88
SN  - 0956-5663
CY  - Amsterdam [u.a.]
PB  - Elsevier Science
M1  - DZNE-2020-05229
SP  - 78-84
PY  - 2017
AB  - Over the last decades, countless bioelectronic monitoring systems were developed for the analysis of cells as well as complex tissues. Most studies addressed the sensitivity and specificity of the bioelectronic detection method in comparison to classical molecular biological assays. In contrast, the up scaling as a prerequisite for the practical application of these novel bioelectronic monitoring systems is mostly only discussed theoretically. In this context, we developed a novel 384-multiwell microelectrode array (MMEA) based measurement system for the sensitive label-free real-time monitoring of neurodegenerative processes by impedance spectroscopy. With respect to the needs of productive screening systems for robust and reproducible measurements on high numbers of plates, we focused on reducing the critical contacting of more than 400 electrodes for a 384-MMEA. Therefore, we introduced an on top array of immersive counter electrodes that are individually addressed by a multiplexer and connected all measurement electrodes on the 384-MMEA to a single contact point. More strikingly, our novel approach provided a comparable signal stability and sensitivity similar to an array with integrated counter electrodes. Next, we optimized a SH-SY5Y cell based tauopathy model by introducing a novel 5-fold Tau mutation eliminating the need of artificial tauopathy induction. In combination with our novel 384-MMEA based measurement system, the concentration and time dependent neuroregenerative effect of the kinase inhibitor SRN-003-556 could be quantitatively monitored. Thus, our novel screening system could be a useful tool to identify and develop potential novel therapeutics in the field of Tau-related neurodegenerative diseases.
KW  - Carbazoles: pharmacology
KW  - Cell Line
KW  - Dielectric Spectroscopy: instrumentation
KW  - Dielectric Spectroscopy: methods
KW  - Drug Evaluation, Preclinical: instrumentation
KW  - Drug Evaluation, Preclinical: methods
KW  - Equipment Design
KW  - Humans
KW  - Microelectrodes
KW  - Tauopathies: diagnosis
KW  - Tauopathies: drug therapy
KW  - tau Proteins: analysis
KW  - Carbazoles (NLM Chemicals)
KW  - SRN 003-556 (NLM Chemicals)
KW  - tau Proteins (NLM Chemicals)
LB  - PUB:(DE-HGF)16
C6  - pmid:27506337
DO  - DOI:10.1016/j.bios.2016.07.074
UR  - https://pub.dzne.de/record/138907
ER  -