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@ARTICLE{Jahnke:138907,
      author       = {Jahnke, Heinz-Georg and Krinke, Dana and Seidel, Diana and
                      Lilienthal, Katharina and Schmidt, Sabine and Azendorf,
                      Ronny and Fischer, Michael and Mack, Till and Striggow,
                      Frank and Althaus, Holger and Schober, Andreas and Robitzki,
                      Andrea A},
      title        = {{A} novel 384-multiwell microelectrode array for the
                      impedimetric monitoring of {T}au protein induced
                      neurodegenerative processes.},
      journal      = {Biosensors and bioelectronics},
      volume       = {88},
      issn         = {0956-5663},
      address      = {Amsterdam [u.a.]},
      publisher    = {Elsevier Science},
      reportid     = {DZNE-2020-05229},
      pages        = {78-84},
      year         = {2017},
      abstract     = {Over the last decades, countless bioelectronic monitoring
                      systems were developed for the analysis of cells as well as
                      complex tissues. Most studies addressed the sensitivity and
                      specificity of the bioelectronic detection method in
                      comparison to classical molecular biological assays. In
                      contrast, the up scaling as a prerequisite for the practical
                      application of these novel bioelectronic monitoring systems
                      is mostly only discussed theoretically. In this context, we
                      developed a novel 384-multiwell microelectrode array (MMEA)
                      based measurement system for the sensitive label-free
                      real-time monitoring of neurodegenerative processes by
                      impedance spectroscopy. With respect to the needs of
                      productive screening systems for robust and reproducible
                      measurements on high numbers of plates, we focused on
                      reducing the critical contacting of more than 400 electrodes
                      for a 384-MMEA. Therefore, we introduced an on top array of
                      immersive counter electrodes that are individually addressed
                      by a multiplexer and connected all measurement electrodes on
                      the 384-MMEA to a single contact point. More strikingly, our
                      novel approach provided a comparable signal stability and
                      sensitivity similar to an array with integrated counter
                      electrodes. Next, we optimized a SH-SY5Y cell based
                      tauopathy model by introducing a novel 5-fold Tau mutation
                      eliminating the need of artificial tauopathy induction. In
                      combination with our novel 384-MMEA based measurement
                      system, the concentration and time dependent
                      neuroregenerative effect of the kinase inhibitor SRN-003-556
                      could be quantitatively monitored. Thus, our novel screening
                      system could be a useful tool to identify and develop
                      potential novel therapeutics in the field of Tau-related
                      neurodegenerative diseases.},
      keywords     = {Carbazoles: pharmacology / Cell Line / Dielectric
                      Spectroscopy: instrumentation / Dielectric Spectroscopy:
                      methods / Drug Evaluation, Preclinical: instrumentation /
                      Drug Evaluation, Preclinical: methods / Equipment Design /
                      Humans / Microelectrodes / Tauopathies: diagnosis /
                      Tauopathies: drug therapy / tau Proteins: analysis /
                      Carbazoles (NLM Chemicals) / SRN 003-556 (NLM Chemicals) /
                      tau Proteins (NLM Chemicals)},
      cin          = {AG Striggow},
      ddc          = {610},
      cid          = {I:(DE-2719)5000045},
      pnm          = {342 - Disease Mechanisms and Model Systems (POF3-342)},
      pid          = {G:(DE-HGF)POF3-342},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:27506337},
      doi          = {10.1016/j.bios.2016.07.074},
      url          = {https://pub.dzne.de/record/138907},
}