TY  - JOUR
AU  - Hofmann, Kristina
AU  - Lamberz, Christian
AU  - Piotrowitz, Kira
AU  - Offermann, Nina
AU  - But, Diana
AU  - Scheller, Anja
AU  - Alamoudi, Ashraf
AU  - Kuerschner, Lars
TI  - Tanycytes and a differential fatty acid metabolism in the hypothalamus.
JO  - Glia
VL  - 65
IS  - 2
SN  - 0894-1491
CY  - Bognor Regis [u.a.]
PB  - Wiley-Liss
M1  - DZNE-2020-05349
SP  - 231-249
PY  - 2017
AB  - Although the brain controls all main metabolic pathways in the whole organism, its lipid metabolism is partially separated from the rest of the body. Circulating lipids and other metabolites are taken up into brain areas like the hypothalamus and are locally metabolized and sensed involving several hypothalamic cell types. In this study we show that saturated and unsaturated fatty acids are differentially processed in the murine hypothalamus. The observed differences involve both lipid distribution and metabolism. Key findings were: (i) hypothalamic astrocytes are targeted by unsaturated, but not saturated lipids in lean mice; (ii) in obese mice labeling of these astrocytes by unsaturated oleic acid cannot be detected unless β-oxidation or ketogenesis is inhibited; (iii) the hypothalamus of obese animals increases ketone body and neutral lipid synthesis while tanycytes, hypothalamic cells facing the ventricle, increase their lipid droplet content; and (iv) tanycytes show different labeling for saturated or unsaturated lipids. Our data support a metabolic connection between tanycytes and astrocytes likely to impact hypothalamic lipid sensing. GLIA 2017;65:231-249.
KW  - Animals
KW  - Astrocytes: metabolism
KW  - Diet, High-Fat: adverse effects
KW  - Disease Models, Animal
KW  - Ependymoglial Cells: metabolism
KW  - Ependymoglial Cells: ultrastructure
KW  - Excitatory Amino Acid Transporter 1: genetics
KW  - Excitatory Amino Acid Transporter 1: metabolism
KW  - Fatty Acids: metabolism
KW  - Glial Fibrillary Acidic Protein: genetics
KW  - Glial Fibrillary Acidic Protein: metabolism
KW  - Hypothalamus: cytology
KW  - Hypothalamus: metabolism
KW  - In Vitro Techniques
KW  - Ketone Bodies: metabolism
KW  - Lipid Metabolism: physiology
KW  - Luminescent Proteins: genetics
KW  - Luminescent Proteins: metabolism
KW  - Male
KW  - Mice
KW  - Mice, Inbred C57BL
KW  - Mice, Transgenic
KW  - Obesity: chemically induced
KW  - Obesity: pathology
KW  - Oligodendrocyte Transcription Factor 2: metabolism
KW  - Organ Culture Techniques
KW  - Excitatory Amino Acid Transporter 1 (NLM Chemicals)
KW  - Fatty Acids (NLM Chemicals)
KW  - Glial Fibrillary Acidic Protein (NLM Chemicals)
KW  - Ketone Bodies (NLM Chemicals)
KW  - Luminescent Proteins (NLM Chemicals)
KW  - Oligodendrocyte Transcription Factor 2 (NLM Chemicals)
KW  - Slc1a3 protein, mouse (NLM Chemicals)
LB  - PUB:(DE-HGF)16
C6  - pmid:27726181
DO  - DOI:10.1002/glia.23088
UR  - https://pub.dzne.de/record/139027
ER  -