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| 001 | 139309 | ||
| 005 | 20240321220609.0 | ||
| 024 | 7 | _ | |a 10.1084/jem.20160933 |2 doi |
| 024 | 7 | _ | |a pmid:28465465 |2 pmid |
| 024 | 7 | _ | |a pmc:PMC5460996 |2 pmc |
| 024 | 7 | _ | |a 0022-1007 |2 ISSN |
| 024 | 7 | _ | |a 1540-9358 |2 ISSN |
| 024 | 7 | _ | |a 1540-9538 |2 ISSN |
| 024 | 7 | _ | |a altmetric:19847353 |2 altmetric |
| 037 | _ | _ | |a DZNE-2020-05631 |
| 041 | _ | _ | |a English |
| 082 | _ | _ | |a 610 |
| 100 | 1 | _ | |a Stutz, Andrea |b 0 |
| 245 | _ | _ | |a NLRP3 inflammasome assembly is regulated by phosphorylation of the pyrin domain. |
| 260 | _ | _ | |a New York, NY |c 2017 |b Rockefeller Univ. Press |
| 264 | _ | 1 | |3 online |2 Crossref |b Rockefeller University Press |c 2017-05-02 |
| 264 | _ | 1 | |3 print |2 Crossref |b Rockefeller University Press |c 2017-06-05 |
| 336 | 7 | _ | |a article |2 DRIVER |
| 336 | 7 | _ | |a Output Types/Journal article |2 DataCite |
| 336 | 7 | _ | |a Journal Article |b journal |m journal |0 PUB:(DE-HGF)16 |s 1591372938_14981 |2 PUB:(DE-HGF) |
| 336 | 7 | _ | |a ARTICLE |2 BibTeX |
| 336 | 7 | _ | |a JOURNAL_ARTICLE |2 ORCID |
| 336 | 7 | _ | |a Journal Article |0 0 |2 EndNote |
| 520 | _ | _ | |a NLRP3 is a cytosolic pattern recognition receptor that senses microbes and endogenous danger signals. Upon activation, NLRP3 forms an inflammasome with the adapter ASC, resulting in caspase-1 activation, release of proinflammatory cytokines and cell death. How NLRP3 activation is regulated by transcriptional and posttranslational mechanisms to prevent aberrant activation remains incompletely understood. Here, we identify three conserved phosphorylation sites in NLRP3 and demonstrate that NLRP3 activation is controlled by phosphorylation of its pyrin domain (PYD). Phosphomimetic residues in NLRP3 PYD abrogate inflammasome activation and structural modeling indicates that phosphorylation of the PYD regulates charge-charge interaction between two PYDs that are essential for NLRP3 activation. Phosphatase 2A (PP2A) inhibition or knock-down drastically reduces NLRP3 activation, showing that PP2A can license inflammasome assembly via dephosphorylating NLRP3 PYD. These results propose that the balance between kinases and phosphatases acting on the NLRP3 PYD is critical for NLRP3 activation. |
| 536 | _ | _ | |a 342 - Disease Mechanisms and Model Systems (POF3-342) |0 G:(DE-HGF)POF3-342 |c POF3-342 |f POF III |x 0 |
| 542 | _ | _ | |i 2019-09-19 |2 Crossref |u http://www.rupress.org/terms/ |
| 542 | _ | _ | |i 2017-12-04 |2 Crossref |u https://creativecommons.org/licenses/by-nc-sa/4.0/ |
| 588 | _ | _ | |a Dataset connected to CrossRef, PubMed, |
| 650 | _ | 7 | |a Inflammasomes |2 NLM Chemicals |
| 650 | _ | 7 | |a NLR Family, Pyrin Domain-Containing 3 Protein |2 NLM Chemicals |
| 650 | _ | 7 | |a Nlrp3 protein, mouse |2 NLM Chemicals |
| 650 | _ | 7 | |a Pyrin |2 NLM Chemicals |
| 650 | _ | 7 | |a Phosphoserine |0 17885-08-4 |2 NLM Chemicals |
| 650 | _ | 7 | |a Protein Phosphatase 2 |0 EC 3.1.3.16 |2 NLM Chemicals |
| 650 | _ | 2 | |a Amino Acid Sequence |2 MeSH |
| 650 | _ | 2 | |a Animals |2 MeSH |
| 650 | _ | 2 | |a HEK293 Cells |2 MeSH |
| 650 | _ | 2 | |a Humans |2 MeSH |
| 650 | _ | 2 | |a Inflammasomes: metabolism |2 MeSH |
| 650 | _ | 2 | |a Mice |2 MeSH |
| 650 | _ | 2 | |a Models, Biological |2 MeSH |
| 650 | _ | 2 | |a Models, Molecular |2 MeSH |
| 650 | _ | 2 | |a NLR Family, Pyrin Domain-Containing 3 Protein: chemistry |2 MeSH |
| 650 | _ | 2 | |a NLR Family, Pyrin Domain-Containing 3 Protein: metabolism |2 MeSH |
| 650 | _ | 2 | |a Phosphorylation |2 MeSH |
| 650 | _ | 2 | |a Phosphoserine: metabolism |2 MeSH |
| 650 | _ | 2 | |a Protein Binding |2 MeSH |
| 650 | _ | 2 | |a Protein Domains |2 MeSH |
| 650 | _ | 2 | |a Protein Phosphatase 2: metabolism |2 MeSH |
| 650 | _ | 2 | |a Pyrin: chemistry |2 MeSH |
| 650 | _ | 2 | |a Structure-Activity Relationship |2 MeSH |
| 700 | 1 | _ | |a Kolbe, Carl-Christian |b 1 |
| 700 | 1 | _ | |a Stahl, Rainer |b 2 |
| 700 | 1 | _ | |a Horvath, Gabor L |b 3 |
| 700 | 1 | _ | |a Franklin, Bernardo S |0 P:(DE-HGF)0 |b 4 |
| 700 | 1 | _ | |a van Ray, Olivia |b 5 |
| 700 | 1 | _ | |a Brinkschulte, Rebecca |b 6 |
| 700 | 1 | _ | |a Geyer, Matthias |b 7 |
| 700 | 1 | _ | |a Meissner, Felix |b 8 |
| 700 | 1 | _ | |a Latz, Eicke |0 P:(DE-2719)2000062 |b 9 |e Last author |u dzne |
| 773 | 1 | 8 | |a 10.1084/jem.20160933 |b : Rockefeller University Press, 2017-05-02 |n 6 |p 1725-1736 |3 journal-article |2 Crossref |t Journal of Experimental Medicine |v 214 |y 2017 |x 0022-1007 |
| 773 | _ | _ | |a 10.1084/jem.20160933 |g Vol. 214, no. 6, p. 1725 - 1736 |0 PERI:(DE-600)1477240-1 |n 6 |q 214:6<1725 - 1736 |p 1725-1736 |t Journal of experimental medicine |v 214 |y 2017 |x 0022-1007 |
| 856 | 7 | _ | |2 Pubmed Central |u http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5460996 |
| 909 | C | O | |o oai:pub.dzne.de:139309 |p VDB |
| 910 | 1 | _ | |a Deutsches Zentrum für Neurodegenerative Erkrankungen |0 I:(DE-588)1065079516 |k DZNE |b 9 |6 P:(DE-2719)2000062 |
| 913 | 1 | _ | |a DE-HGF |b Forschungsbereich Gesundheit |l Erkrankungen des Nervensystems |1 G:(DE-HGF)POF3-340 |0 G:(DE-HGF)POF3-342 |2 G:(DE-HGF)POF3-300 |v Disease Mechanisms and Model Systems |x 0 |
| 914 | 1 | _ | |y 2017 |
| 915 | _ | _ | |a JCR |0 StatID:(DE-HGF)0100 |2 StatID |b J EXP MED : 2021 |d 2022-11-13 |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)0200 |2 StatID |b SCOPUS |d 2022-11-13 |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)0300 |2 StatID |b Medline |d 2022-11-13 |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)0199 |2 StatID |b Clarivate Analytics Master Journal List |d 2022-11-13 |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)0150 |2 StatID |b Web of Science Core Collection |d 2022-11-13 |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)1050 |2 StatID |b BIOSIS Previews |d 2022-11-13 |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)1030 |2 StatID |b Current Contents - Life Sciences |d 2022-11-13 |
| 915 | _ | _ | |a IF >= 15 |0 StatID:(DE-HGF)9915 |2 StatID |b J EXP MED : 2021 |d 2022-11-13 |
| 920 | 1 | _ | |0 I:(DE-2719)1013024 |k AG Latz |l Innate Immunity in Neurodegeneration |x 0 |
| 980 | _ | _ | |a journal |
| 980 | _ | _ | |a VDB |
| 980 | _ | _ | |a I:(DE-2719)1013024 |
| 980 | _ | _ | |a UNRESTRICTED |
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