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@ARTICLE{deBoni:139768,
      author       = {de Boni, Laura and Gasparoni, Gilles and Haubenreich,
                      Carolin and Tierling, Sascha and Schmitt, Ina and Peitz,
                      Michael and Koch, Philipp and Walter, Jörn and Wüllner,
                      Ullrich and Brüstle, Oliver},
      title        = {{DNA} methylation alterations in i{PSC}- and h{ESC}-derived
                      neurons: potential implications for neurological disease
                      modeling.},
      journal      = {Clinical epigenetics},
      volume       = {10},
      number       = {1},
      issn         = {1868-7075},
      address      = {[S.l.]},
      publisher    = {BioMed Central},
      reportid     = {DZNE-2020-06090},
      pages        = {13},
      year         = {2018},
      abstract     = {Genetic predisposition and epigenetic alterations are both
                      considered to contribute to sporadic neurodegenerative
                      diseases (NDDs) such as Parkinson's disease (PD). Since cell
                      reprogramming and the generation of induced pluripotent stem
                      cells (iPSCs) are themselves associated with major
                      epigenetic remodeling, it remains unclear to what extent
                      iPSC-derived neurons lend themselves to model epigenetic
                      disease-associated changes. A key question to be addressed
                      in this context is whether iPSC-derived neurons exhibit
                      epigenetic signatures typically observed in neurons derived
                      from non-reprogrammed human embryonic stem cells
                      (hESCs).Here, we compare mature neurons derived from hESC
                      and isogenic human iPSC generated from hESC-derived neural
                      stem cells. Genome-wide 450 K-based DNA methylation and
                      HT12v4 gene array expression analyses were complemented by a
                      deep analysis of selected genes known to be involved in NDD.
                      Our studies show that DNA methylation and gene expression
                      patterns of isogenic hESC- and iPSC-derived neurons are
                      markedly preserved on a genome-wide and single gene
                      level.Overall, iPSC-derived neurons exhibit similar DNA
                      methylation patterns compared to isogenic hESC-derived
                      neurons. Further studies will be required to explore whether
                      the epigenetic patterns observed in iPSC-derived neurons
                      correspond to those detectable in native brain neurons.},
      keywords     = {Cell Differentiation / Cells, Cultured / DNA Methylation /
                      Epigenesis, Genetic / Gene Expression Profiling / Human
                      Embryonic Stem Cells: chemistry / Human Embryonic Stem
                      Cells: cytology / Humans / Induced Pluripotent Stem Cells:
                      chemistry / Induced Pluripotent Stem Cells: cytology /
                      Neurons: chemistry / Oligonucleotide Array Sequence Analysis
                      / Sequence Analysis, DNA / alpha-Synuclein: genetics / SNCA
                      protein, human (NLM Chemicals) / alpha-Synuclein (NLM
                      Chemicals)},
      cin          = {AG Wüllner / Cell Programming Unit},
      ddc          = {610},
      cid          = {I:(DE-2719)1011302 / I:(DE-2719)1013013},
      pnm          = {344 - Clinical and Health Care Research (POF3-344)},
      pid          = {G:(DE-HGF)POF3-344},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:29422978},
      pmc          = {pmc:PMC5789607},
      doi          = {10.1186/s13148-018-0440-0},
      url          = {https://pub.dzne.de/record/139768},
}