Home > Publications Database > Altered mitochondrial bioenergetics and ultrastructure in the skeletal muscle of young adults with type 1 diabetes. > print

001     139937
005     20240409144224.0
024 7 _ |a 10.1007/s00125-018-4602-6
|2 doi
024 7 _ |a pmid:29666899
|2 pmid
024 7 _ |a 0012-186X
|2 ISSN
024 7 _ |a 1432-0428
|2 ISSN
024 7 _ |a altmetric:37945056
|2 altmetric
037 _ _ |a DZNE-2020-06259
041 _ _ |a English
082 _ _ |a 610
100 1 _ |a Monaco, Cynthia M F
|b 0
245 _ _ |a Altered mitochondrial bioenergetics and ultrastructure in the skeletal muscle of young adults with type 1 diabetes.
260 _ _ |a Heidelberg
|c 2018
|b Springer
264 _ 1 |3 online
|2 Crossref
|b Springer Science and Business Media LLC
|c 2018-04-18
264 _ 1 |3 print
|2 Crossref
|b Springer Science and Business Media LLC
|c 2018-06-01
336 7 _ |a article
|2 DRIVER
336 7 _ |a Output Types/Journal article
|2 DataCite
336 7 _ |a Journal Article
|b journal
|m journal
|0 PUB:(DE-HGF)16
|s 1712666497_12209
|2 PUB:(DE-HGF)
336 7 _ |a ARTICLE
|2 BibTeX
336 7 _ |a JOURNAL_ARTICLE
|2 ORCID
336 7 _ |a Journal Article
|0 0
|2 EndNote
520 _ _ |a A comprehensive assessment of skeletal muscle ultrastructure and mitochondrial bioenergetics has not been undertaken in individuals with type 1 diabetes. This study aimed to systematically assess skeletal muscle mitochondrial phenotype in young adults with type 1 diabetes.Physically active, young adults (men and women) with type 1 diabetes (HbA1c 63.0 ± 16.0 mmol/mol [7.9% ± 1.5%]) and without type 1 diabetes (control), matched for sex, age, BMI and level of physical activity, were recruited (n = 12/group) to undergo vastus lateralis muscle microbiopsies. Mitochondrial respiration (high-resolution respirometry), site-specific mitochondrial H2O2 emission and Ca2+ retention capacity (CRC) (spectrofluorometry) were assessed using permeabilised myofibre bundles. Electron microscopy and tomography were used to quantify mitochondrial content and investigate muscle ultrastructure. Skeletal muscle microvasculature was assessed by immunofluorescence.Mitochondrial oxidative capacity was significantly lower in participants with type 1 diabetes vs the control group, specifically at Complex II of the electron transport chain, without differences in mitochondrial content between groups. Muscles of those with type 1 diabetes also exhibited increased mitochondrial H2O2 emission at Complex III and decreased CRC relative to control individuals. Electron tomography revealed an increase in the size and number of autophagic remnants in the muscles of participants with type 1 diabetes. Despite this, levels of the autophagic regulatory protein, phosphorylated AMP-activated protein kinase (p-AMPKαThr172), and its downstream targets, phosphorylated Unc-51 like autophagy activating kinase 1 (p-ULK1Ser555) and p62, was similar between groups. In addition, no differences in muscle capillary density or platelet aggregation were observed between the groups.Alterations in mitochondrial ultrastructure and bioenergetics are evident within the skeletal muscle of active young adults with type 1 diabetes. It is yet to be elucidated whether more rigorous exercise may help to prevent skeletal muscle metabolic deficiencies in both active and inactive individuals with type 1 diabetes.
536 _ _ |a 341 - Molecular Signaling (POF3-341)
|0 G:(DE-HGF)POF3-341
|c POF3-341
|f POF III
|x 0
542 _ _ |i 2018-04-18
|2 Crossref
|u http://www.springer.com/tdm
588 _ _ |a Dataset connected to CrossRef, PubMed,
650 _ 7 |a Insulin
|2 NLM Chemicals
650 _ 7 |a Hydrogen Peroxide
|0 BBX060AN9V
|2 NLM Chemicals
650 _ 7 |a Calcium
|0 SY7Q814VUP
|2 NLM Chemicals
650 _ 2 |a Adult
|2 MeSH
650 _ 2 |a Body Mass Index
|2 MeSH
650 _ 2 |a Calcium: chemistry
|2 MeSH
650 _ 2 |a Diabetes Mellitus, Type 1: metabolism
|2 MeSH
650 _ 2 |a Diabetes Mellitus, Type 1: pathology
|2 MeSH
650 _ 2 |a Energy Metabolism
|2 MeSH
650 _ 2 |a Exercise: physiology
|2 MeSH
650 _ 2 |a Female
|2 MeSH
650 _ 2 |a Humans
|2 MeSH
650 _ 2 |a Hydrogen Peroxide: metabolism
|2 MeSH
650 _ 2 |a Insulin: metabolism
|2 MeSH
650 _ 2 |a Male
|2 MeSH
650 _ 2 |a Microscopy, Electron, Transmission
|2 MeSH
650 _ 2 |a Microscopy, Fluorescence
|2 MeSH
650 _ 2 |a Mitochondria: metabolism
|2 MeSH
650 _ 2 |a Mitochondria: ultrastructure
|2 MeSH
650 _ 2 |a Muscle, Skeletal: metabolism
|2 MeSH
650 _ 2 |a Muscle, Skeletal: pathology
|2 MeSH
650 _ 2 |a Muscle, Skeletal: ultrastructure
|2 MeSH
650 _ 2 |a Oxygen Consumption
|2 MeSH
650 _ 2 |a Young Adult
|2 MeSH
700 1 _ |a Hughes, Meghan C
|b 1
700 1 _ |a Ramos, Sofhia V
|b 2
700 1 _ |a Varah, Nina E
|b 3
700 1 _ |a Lamberz, Christian
|0 P:(DE-2719)2811203
|b 4
|u dzne
700 1 _ |a Rahman, Fasih A
|b 5
700 1 _ |a McGlory, Chris
|b 6
700 1 _ |a Tarnopolsky, Mark A
|b 7
700 1 _ |a Krause, Matthew P
|b 8
700 1 _ |a Laham, Robert
|b 9
700 1 _ |a Hawke, Thomas J
|0 P:(DE-HGF)0
|b 10
|e Corresponding author
700 1 _ |a Perry, Christopher G R
|b 11
773 1 8 |a 10.1007/s00125-018-4602-6
|b : Springer Science and Business Media LLC, 2018-04-18
|n 6
|p 1411-1423
|3 journal-article
|2 Crossref
|t Diabetologia
|v 61
|y 2018
|x 0012-186X
773 _ _ |a 10.1007/s00125-018-4602-6
|g Vol. 61, no. 6, p. 1411 - 1423
|0 PERI:(DE-600)1458993-X
|n 6
|q 61:6<1411 - 1423
|p 1411-1423
|t Diabetologia
|v 61
|y 2018
|x 0012-186X
856 4 _ |u https://pub.dzne.de/record/139937/files/DZNE-2020-06259_Restricted.pdf
856 4 _ |u https://pub.dzne.de/record/139937/files/DZNE-2020-06259_Restricted.pdf?subformat=pdfa
|x pdfa
909 C O |p VDB
|o oai:pub.dzne.de:139937
910 1 _ |a Deutsches Zentrum für Neurodegenerative Erkrankungen
|0 I:(DE-588)1065079516
|k DZNE
|b 4
|6 P:(DE-2719)2811203
913 1 _ |a DE-HGF
|b Gesundheit
|l Erkrankungen des Nervensystems
|1 G:(DE-HGF)POF3-340
|0 G:(DE-HGF)POF3-341
|3 G:(DE-HGF)POF3
|2 G:(DE-HGF)POF3-300
|4 G:(DE-HGF)POF
|v Molecular Signaling
|x 0
914 1 _ |y 2018
915 _ _ |a JCR
|0 StatID:(DE-HGF)0100
|2 StatID
|b DIABETOLOGIA : 2021
|d 2022-11-08
915 _ _ |a DBCoverage
|0 StatID:(DE-HGF)0200
|2 StatID
|b SCOPUS
|d 2022-11-08
915 _ _ |a DBCoverage
|0 StatID:(DE-HGF)0300
|2 StatID
|b Medline
|d 2022-11-08
915 _ _ |a DBCoverage
|0 StatID:(DE-HGF)0199
|2 StatID
|b Clarivate Analytics Master Journal List
|d 2022-11-08
915 _ _ |a DBCoverage
|0 StatID:(DE-HGF)0150
|2 StatID
|b Web of Science Core Collection
|d 2022-11-08
915 _ _ |a DBCoverage
|0 StatID:(DE-HGF)1050
|2 StatID
|b BIOSIS Previews
|d 2022-11-08
915 _ _ |a DBCoverage
|0 StatID:(DE-HGF)1110
|2 StatID
|b Current Contents - Clinical Medicine
|d 2022-11-08
915 _ _ |a DBCoverage
|0 StatID:(DE-HGF)1030
|2 StatID
|b Current Contents - Life Sciences
|d 2022-11-08
915 _ _ |a IF >= 10
|0 StatID:(DE-HGF)9910
|2 StatID
|b DIABETOLOGIA : 2021
|d 2022-11-08
920 1 _ |0 I:(DE-2719)1013012
|k AG Alamoudi
|l Cryo-electron microscopy and tomography
|x 0
980 _ _ |a journal
980 _ _ |a VDB
980 _ _ |a I:(DE-2719)1013012
980 _ _ |a UNRESTRICTED
999 C 5 |9 -- missing cx lookup --
|a 10.2337/diab.31.9.795
|2 Crossref
|o 10.2337/diab.31.9.795
999 C 5 |9 -- missing cx lookup --
|a 10.1056/NEJM199001253220403
|2 Crossref
|o 10.1056/NEJM199001253220403
999 C 5 |y 1997
|2 Crossref
|o J Jensen 1997
999 C 5 |9 -- missing cx lookup --
|a 10.2174/1573399810602040375
|2 Crossref
|o 10.2174/1573399810602040375
999 C 5 |9 -- missing cx lookup --
|a 10.1056/NEJM199309303291401
|2 Crossref
|o 10.1056/NEJM199309303291401
999 C 5 |9 -- missing cx lookup --
|a 10.1056/NEJM200002103420603
|2 Crossref
|o 10.1056/NEJM200002103420603
999 C 5 |9 -- missing cx lookup --
|a 10.1016/S2213-8587(13)70046-9
|2 Crossref
|o 10.1016/S2213-8587(13)70046-9
999 C 5 |9 -- missing cx lookup --
|a 10.1016/j.cmet.2012.04.010
|2 Crossref
|o 10.1016/j.cmet.2012.04.010
999 C 5 |9 -- missing cx lookup --
|a 10.2337/db14-0765
|2 Crossref
|o 10.2337/db14-0765
999 C 5 |9 -- missing cx lookup --
|a 10.1111/j.1365-2796.2010.02298.x
|2 Crossref
|o 10.1111/j.1365-2796.2010.02298.x
999 C 5 |9 -- missing cx lookup --
|a 10.1152/ajpendo.00343.2002
|2 Crossref
|o 10.1152/ajpendo.00343.2002
999 C 5 |9 -- missing cx lookup --
|a 10.1111/j.1600-0404.1977.tb05654.x
|2 Crossref
|o 10.1111/j.1600-0404.1977.tb05654.x
999 C 5 |9 -- missing cx lookup --
|a 10.3389/fphys.2015.00360
|2 Crossref
|o 10.3389/fphys.2015.00360
999 C 5 |9 -- missing cx lookup --
|a 10.2337/db15-1577
|2 Crossref
|o 10.2337/db15-1577
999 C 5 |9 -- missing cx lookup --
|a 10.1007/978-3-662-04162-8_45
|2 Crossref
|o 10.1007/978-3-662-04162-8_45
999 C 5 |9 -- missing cx lookup --
|a 10.3390/ijms12129296
|2 Crossref
|o 10.3390/ijms12129296
999 C 5 |9 -- missing cx lookup --
|a 10.1042/BJ20110366
|2 Crossref
|o 10.1042/BJ20110366
999 C 5 |9 -- missing cx lookup --
|a 10.2337/db12-1219
|2 Crossref
|o 10.2337/db12-1219
999 C 5 |9 -- missing cx lookup --
|a 10.1152/ajpheart.00701.2003
|2 Crossref
|o 10.1152/ajpheart.00701.2003
999 C 5 |9 -- missing cx lookup --
|a 10.1042/BJ20141447
|2 Crossref
|o 10.1042/BJ20141447
999 C 5 |9 -- missing cx lookup --
|a 10.1074/jbc.R117.789271
|2 Crossref
|o 10.1074/jbc.R117.789271
999 C 5 |9 -- missing cx lookup --
|a 10.1016/j.freeradbiomed.2013.09.008
|2 Crossref
|o 10.1016/j.freeradbiomed.2013.09.008
999 C 5 |9 -- missing cx lookup --
|a 10.1152/ajpheart.00932.2010
|2 Crossref
|o 10.1152/ajpheart.00932.2010
999 C 5 |9 -- missing cx lookup --
|a 10.1016/j.freeradbiomed.2015.05.019
|2 Crossref
|o 10.1016/j.freeradbiomed.2015.05.019
999 C 5 |y 2007
|2 Crossref
|o MA Tarnopolsky 2007
999 C 5 |9 -- missing cx lookup --
|a 10.1006/jsbi.1996.0013
|2 Crossref
|o 10.1006/jsbi.1996.0013
999 C 5 |9 -- missing cx lookup --
|a 10.1016/j.mce.2012.06.019
|2 Crossref
|o 10.1016/j.mce.2012.06.019
999 C 5 |9 -- missing cx lookup --
|a 10.1038/nature03434
|2 Crossref
|o 10.1038/nature03434
999 C 5 |9 -- missing cx lookup --
|a 10.2337/dc09-0354
|2 Crossref
|o 10.2337/dc09-0354
999 C 5 |9 -- missing cx lookup --
|a 10.2337/dc16-1728
|2 Crossref
|o 10.2337/dc16-1728
999 C 5 |9 -- missing cx lookup --
|a 10.1016/j.jcjd.2013.01.018
|2 Crossref
|o 10.1016/j.jcjd.2013.01.018
999 C 5 |9 -- missing cx lookup --
|a 10.1046/j.1440-1681.2000.03225.x
|2 Crossref
|o 10.1046/j.1440-1681.2000.03225.x
999 C 5 |9 -- missing cx lookup --
|a 10.2337/diab.41.2.S102
|2 Crossref
|o 10.2337/diab.41.2.S102
999 C 5 |9 -- missing cx lookup --
|a 10.2337/dc06-1982
|2 Crossref
|o 10.2337/dc06-1982
999 C 5 |9 -- missing cx lookup --
|a 10.1007/s00125-005-0120-4
|2 Crossref
|o 10.1007/s00125-005-0120-4
999 C 5 |9 -- missing cx lookup --
|a 10.1111/j.1749-6632.2000.tb06520.x
|2 Crossref
|o 10.1111/j.1749-6632.2000.tb06520.x
999 C 5 |9 -- missing cx lookup --
|a 10.1080/07853890410017467
|2 Crossref
|o 10.1080/07853890410017467
999 C 5 |9 -- missing cx lookup --
|a 10.4239/wjd.v6.i17.1323
|2 Crossref
|o 10.4239/wjd.v6.i17.1323
999 C 5 |y 2013
|2 Crossref
|o DM D’Souza 2013
999 C 5 |9 -- missing cx lookup --
|a 10.1097/WCO.0000000000000479
|2 Crossref
|o 10.1097/WCO.0000000000000479
999 C 5 |y 2011
|2 Crossref
|o F Item 2011
999 C 5 |9 -- missing cx lookup --
|a 10.1177/1358863X9600100303
|2 Crossref
|o 10.1177/1358863X9600100303
999 C 5 |9 -- missing cx lookup --
|a 10.1074/jbc.M109.026203
|2 Crossref
|o 10.1074/jbc.M109.026203
999 C 5 |9 -- missing cx lookup --
|a 10.1074/jbc.M207217200
|2 Crossref
|o 10.1074/jbc.M207217200
999 C 5 |9 -- missing cx lookup --
|a 10.1016/j.bbabio.2012.12.002
|2 Crossref
|o 10.1016/j.bbabio.2012.12.002
999 C 5 |9 -- missing cx lookup --
|a 10.1038/s41467-017-02746-z
|2 Crossref
|o 10.1038/s41467-017-02746-z
999 C 5 |9 -- missing cx lookup --
|a 10.2337/db07-1056
|2 Crossref
|o 10.2337/db07-1056
999 C 5 |9 -- missing cx lookup --
|a 10.1016/j.freeradbiomed.2009.01.022
|2 Crossref
|o 10.1016/j.freeradbiomed.2009.01.022
999 C 5 |9 -- missing cx lookup --
|a 10.2337/db15-0071
|2 Crossref
|o 10.2337/db15-0071
999 C 5 |9 -- missing cx lookup --
|a 10.2337/diabetes.54.6.1615
|2 Crossref
|o 10.2337/diabetes.54.6.1615

LibraryCollectionCLSMajorCLSMinorLanguageAuthor
Marc 21