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@ARTICLE{Kiss:140090,
      author       = {Kiss, Alexa and Fischer, Irmgard and Kleele, Tatjana and
                      Misgeld, Thomas and Propst, Friedrich},
      title        = {{N}euronal {G}rowth {C}one {S}ize-{D}ependent and
                      -{I}ndependent {P}arameters of {M}icrotubule
                      {P}olymerization.},
      journal      = {Frontiers in cellular neuroscience},
      volume       = {12},
      issn         = {1662-5102},
      address      = {Lausanne},
      publisher    = {Frontiers Research Foundation},
      reportid     = {DZNE-2020-06412},
      pages        = {195},
      year         = {2018},
      abstract     = {Migration and pathfinding of neuronal growth cones during
                      neurite extension is critically dependent on dynamic
                      microtubules. In this study we sought to determine, which
                      aspects of microtubule polymerization relate to growth cone
                      morphology and migratory characteristics. We conducted a
                      multiscale quantitative microscopy analysis using automated
                      tracking of microtubule plus ends in migrating growth cones
                      of cultured murine dorsal root ganglion (DRG) neurons.
                      Notably, this comprehensive analysis failed to identify any
                      changes in microtubule polymerization parameters that were
                      specifically associated with spontaneous extension vs.
                      retraction of growth cones. This suggests that microtubule
                      dynamicity is a basic mechanism that does not determine the
                      polarity of growth cone response but can be exploited to
                      accommodate diverse growth cone behaviors. At the same time,
                      we found a correlation between growth cone size and basic
                      parameters of microtubule polymerization including the
                      density of growing microtubule plus ends and rate and
                      duration of microtubule growth. A similar correlation was
                      observed in growth cones of neurons lacking the
                      microtubule-associated protein MAP1B. However, MAP1B-null
                      growth cones, which are deficient in growth cone migration
                      and steering, displayed an overall reduction in microtubule
                      dynamicity. Our results highlight the importance of taking
                      growth cone size into account when evaluating the influence
                      on growth cone microtubule dynamics of different substrata,
                      guidance factors or genetic manipulations which all can
                      change growth cone morphology and size. The type of large
                      scale multiparametric analysis performed here can help to
                      separate direct effects that these perturbations might have
                      on microtubule dynamics from indirect effects resulting from
                      perturbation-induced changes in growth cone size.},
      cin          = {München Pre 2020 / AG Misgeld},
      ddc          = {610},
      cid          = {I:(DE-2719)6000016 / I:(DE-2719)1110000-4},
      pnm          = {341 - Molecular Signaling (POF3-341)},
      pid          = {G:(DE-HGF)POF3-341},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:30065631},
      pmc          = {pmc:PMC6056669},
      doi          = {10.3389/fncel.2018.00195},
      url          = {https://pub.dzne.de/record/140090},
}