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@ARTICLE{Zhang:140151,
      author       = {Zhang, William H and Herde, Michel K and Mitchell, Joshua A
                      and Whitfield, Jason H and Wulff, Andreas B and Vongsouthi,
                      Vanessa and Sanchez-Romero, Inmaculada and Gulakova, Polina
                      E and Minge, Daniel and Breithausen, Björn and Schoch,
                      Susanne and Janovjak, Harald and Jackson, Colin J and
                      Henneberger, Christian},
      title        = {{M}onitoring hippocampal glycine with the computationally
                      designed optical sensor {G}ly{FS}.},
      journal      = {Nature chemical biology},
      volume       = {14},
      number       = {9},
      issn         = {1552-4450},
      address      = {Basingstoke},
      publisher    = {Nature Publishing Group},
      reportid     = {DZNE-2020-06473},
      pages        = {861-869},
      year         = {2018},
      abstract     = {Fluorescent sensors are an essential part of the
                      experimental toolbox of the life sciences, where they are
                      used ubiquitously to visualize intra- and extracellular
                      signaling. In the brain, optical neurotransmitter sensors
                      can shed light on temporal and spatial aspects of signal
                      transmission by directly observing, for instance,
                      neurotransmitter release and spread. Here we report the
                      development and application of the first optical sensor for
                      the amino acid glycine, which is both an inhibitory
                      neurotransmitter and a co-agonist of the
                      N-methyl-D-aspartate receptors (NMDARs) involved in synaptic
                      plasticity. Computational design of a glycine-specific
                      binding protein allowed us to produce the optical glycine
                      FRET sensor (GlyFS), which can be used with single and
                      two-photon excitation fluorescence microscopy. We took
                      advantage of this newly developed sensor to test predictions
                      about the uneven spatial distribution of glycine in
                      extracellular space and to demonstrate that extracellular
                      glycine levels are controlled by plasticity-inducing
                      stimuli.},
      keywords     = {Animals / Cells, Cultured / Fluorescence Resonance Energy
                      Transfer / Fluorescent Dyes: chemical synthesis /
                      Fluorescent Dyes: chemistry / Glycine: analysis / HEK293
                      Cells / Hippocampus: chemistry / Humans / Male / Optical
                      Imaging / Rats / Rats, Wistar / Fluorescent Dyes (NLM
                      Chemicals) / Glycine (NLM Chemicals)},
      cin          = {U Preclinical Researchers - Bonn},
      ddc          = {570},
      cid          = {I:(DE-2719)7000005},
      pnm          = {342 - Disease Mechanisms and Model Systems (POF3-342)},
      pid          = {G:(DE-HGF)POF3-342},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:30061718},
      doi          = {10.1038/s41589-018-0108-2},
      url          = {https://pub.dzne.de/record/140151},
}