TY  - JOUR
AU  - Ferrari, María E
AU  - Bernis, María E
AU  - McLeod, Faye
AU  - Podpolny, Marina
AU  - Coullery, Romina P
AU  - Casadei, Inelia M
AU  - Salinas, Patricia C
AU  - Rosso, Silvana B
TI  - Wnt7b signalling through Frizzled-7 receptor promotes dendrite development by coactivating CaMKII and JNK.
JO  - Journal of cell science
VL  - 131
IS  - 13
SN  - 0021-9533
CY  - Cambridge
PB  - Company of Biologists Limited
M1  - DZNE-2020-06498
SP  - jcs216101
PY  - 2018
AB  - The formation of complex dendritic arbors is crucial for the assembly of functional networks as abnormal dendrite formation underlies several neurodevelopmental and psychiatric disorders. Many extracellular factors have been postulated as regulators of dendritic growth. Wnt proteins play a critical role in neuronal development and circuit formation. We previously demonstrated that Wnt7b acts through the scaffold protein dishevelled 1 (Dvl1) to modulate dendrite arborisation by activating a non-canonical Wnt signalling pathway. Here, we identify the seven-transmembrane frizzled-7 (Fz7, also known as FZD7) as the receptor for Wnt7b-mediated dendrite growth and complexity. Importantly, Fz7 is developmentally regulated in the intact hippocampus, and is localised along neurites and at dendritic growth cones, suggesting a role in dendrite formation and maturation. Fz7 loss-of-function studies demonstrated that Wnt7b requires Fz7 to promote dendritic arborisation. Moreover, in vivo Fz7 loss of function results in dendritic defects in the intact mouse hippocampus. Furthermore, our findings reveal that Wnt7b and Fz7 induce the phosphorylation of Ca2+/calmodulin-dependent protein kinase II (CaMKII) and JNK proteins, which are required for dendritic development. Here, we demonstrate that Wnt7b-Fz7 signals through two non-canonical Wnt pathways to modulate dendritic growth and complexity.
KW  - Animals
KW  - Calcium-Calmodulin-Dependent Protein Kinase Type 2: genetics
KW  - Calcium-Calmodulin-Dependent Protein Kinase Type 2: metabolism
KW  - Dendrites: enzymology
KW  - Dendrites: genetics
KW  - Dendrites: metabolism
KW  - Dishevelled Proteins: genetics
KW  - Dishevelled Proteins: metabolism
KW  - Hippocampus: growth & development
KW  - Hippocampus: metabolism
KW  - MAP Kinase Kinase 4: genetics
KW  - MAP Kinase Kinase 4: metabolism
KW  - Mice
KW  - Mice, Inbred C57BL
KW  - Neurites: metabolism
KW  - Protein Binding
KW  - Proto-Oncogene Proteins: genetics
KW  - Proto-Oncogene Proteins: metabolism
KW  - Rats
KW  - Rats, Wistar
KW  - Receptors, G-Protein-Coupled: genetics
KW  - Receptors, G-Protein-Coupled: metabolism
KW  - Wnt Proteins: genetics
KW  - Wnt Proteins: metabolism
KW  - Wnt Signaling Pathway
KW  - Dishevelled Proteins (NLM Chemicals)
KW  - Dvl1 protein, mouse (NLM Chemicals)
KW  - Fzd7 protein, mouse (NLM Chemicals)
KW  - Proto-Oncogene Proteins (NLM Chemicals)
KW  - Receptors, G-Protein-Coupled (NLM Chemicals)
KW  - Wnt Proteins (NLM Chemicals)
KW  - Wnt7b protein, mouse (NLM Chemicals)
KW  - Calcium-Calmodulin-Dependent Protein Kinase Type 2 (NLM Chemicals)
KW  - MAP Kinase Kinase 4 (NLM Chemicals)
LB  - PUB:(DE-HGF)16
C6  - pmid:29898920
DO  - DOI:10.1242/jcs.216101
UR  - https://pub.dzne.de/record/140176
ER  -