000140214 001__ 140214
000140214 005__ 20240501120100.0
000140214 0247_ $$2doi$$a10.1038/s41598-018-32581-1
000140214 0247_ $$2pmid$$apmid:30242186
000140214 0247_ $$2pmc$$apmc:PMC6155003
000140214 0247_ $$2altmetric$$aaltmetric:49943838
000140214 037__ $$aDZNE-2020-06536
000140214 041__ $$aEnglish
000140214 082__ $$a600
000140214 1001_ $$0P:(DE-2719)2810494$$aWüsten, Annick$$b0$$eFirst author$$udzne
000140214 245__ $$aA Bioluminescent Cell Assay to Quantify Prion Protein Dimerization.
000140214 260__ $$a[London]$$bMacmillan Publishers Limited, part of Springer Nature$$c2018
000140214 264_1 $$2Crossref$$3online$$bSpringer Science and Business Media LLC$$c2018-09-21
000140214 264_1 $$2Crossref$$3print$$bSpringer Science and Business Media LLC$$c2018-12-01
000140214 3367_ $$2DRIVER$$aarticle
000140214 3367_ $$2DataCite$$aOutput Types/Journal article
000140214 3367_ $$0PUB:(DE-HGF)16$$2PUB:(DE-HGF)$$aJournal Article$$bjournal$$mjournal$$s1714481609_8224
000140214 3367_ $$2BibTeX$$aARTICLE
000140214 3367_ $$2ORCID$$aJOURNAL_ARTICLE
000140214 3367_ $$00$$2EndNote$$aJournal Article
000140214 520__ $$aThe prion protein (PrP) is a cell surface protein that in disease misfolds and becomes infectious causing Creutzfeldt-Jakob disease in humans, scrapie in sheep, and chronic wasting disease in deer and elk. Little is known regarding the dimerization of PrP and its role in disease. We developed a bioluminescent prion assay (BPA) to quantify PrP dimerization by bimolecular complementation of split Gaussia luciferase (GLuc) halves that are each fused to PrP. Fusion constructs between PrP and N- and C-terminal GLuc halves were expressed on the surface of RK13 cells (RK13-DC cells) and dimerized to yield a bioluminescent signal that was decreased in the presence of eight different antibodies to PrP. Dimerization of PrP was independent of divalent cations and was induced under stress. Challenge of RK13-DC cells with seven different prion strains did not lead to detectable infection but was measurable by bioluminescence. Finally, we used BPA to screen a compound library for compounds inhibiting PrP dimerization. One of the most potent compounds to inhibit PrP dimerization was JTC-801, which also inhibited prion replication in RML-infected ScN2a and SMB cells with an EC50 of 370 nM and 220 nM, respectively. We show here that BPA is a versatile tool to study prion biology and to identify anti-prion compounds.
000140214 536__ $$0G:(DE-HGF)POF3-342$$a342 - Disease Mechanisms and Model Systems (POF3-342)$$cPOF3-342$$fPOF III$$x0
000140214 542__ $$2Crossref$$i2018-09-21$$uhttps://creativecommons.org/licenses/by/4.0
000140214 588__ $$aDataset connected to CrossRef, PubMed,
000140214 650_7 $$2NLM Chemicals$$aCations, Divalent
000140214 650_7 $$2NLM Chemicals$$aPrion Proteins
000140214 650_2 $$2MeSH$$aAnimals
000140214 650_2 $$2MeSH$$aBiological Assay: methods
000140214 650_2 $$2MeSH$$aCations, Divalent: metabolism
000140214 650_2 $$2MeSH$$aCell Line
000140214 650_2 $$2MeSH$$aCell Line, Tumor
000140214 650_2 $$2MeSH$$aCreutzfeldt-Jakob Syndrome: metabolism
000140214 650_2 $$2MeSH$$aDeer
000140214 650_2 $$2MeSH$$aDimerization
000140214 650_2 $$2MeSH$$aHumans
000140214 650_2 $$2MeSH$$aLuminescent Measurements: methods
000140214 650_2 $$2MeSH$$aMice
000140214 650_2 $$2MeSH$$aPrion Proteins: metabolism
000140214 650_2 $$2MeSH$$aProtein Folding
000140214 650_2 $$2MeSH$$aRabbits
000140214 650_2 $$2MeSH$$aScrapie: metabolism
000140214 650_2 $$2MeSH$$aSheep
000140214 650_2 $$2MeSH$$aWasting Disease, Chronic: metabolism
000140214 7001_ $$0P:(DE-2719)2811529$$aPasham Parameshwar, Reddy$$b1$$udzne
000140214 7001_ $$0P:(DE-2719)2811502$$aSmiyakin, Andrej$$b2$$udzne
000140214 7001_ $$0P:(DE-2719)2810557$$aBernis, Maria Eugenia$$b3$$udzne
000140214 7001_ $$0P:(DE-2719)2810376$$aTamgüney, Gültekin$$b4$$eLast author$$udzne
000140214 77318 $$2Crossref$$3journal-article$$a10.1038/s41598-018-32581-1$$b : Springer Science and Business Media LLC, 2018-09-21$$n1$$p14178$$tScientific Reports$$v8$$x2045-2322$$y2018
000140214 773__ $$0PERI:(DE-600)2615211-3$$a10.1038/s41598-018-32581-1$$gVol. 8, no. 1, p. 14178$$n1$$p14178$$q8:1<14178$$tScientific reports$$v8$$x2045-2322$$y2018
000140214 8564_ $$uhttps://pub.dzne.de/record/140214/files/DZNE-2020-06536.pdf$$yOpenAccess
000140214 8564_ $$uhttps://pub.dzne.de/record/140214/files/DZNE-2020-06536.pdf?subformat=pdfa$$xpdfa$$yOpenAccess
000140214 8567_ $$2Pubmed Central$$uhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC6155003
000140214 909CO $$ooai:pub.dzne.de:140214$$pdnbdelivery$$pdriver$$popenaire$$pVDB$$popen_access
000140214 9101_ $$0I:(DE-588)1065079516$$6P:(DE-2719)2810494$$aDeutsches Zentrum für Neurodegenerative Erkrankungen$$b0$$kDZNE
000140214 9101_ $$0I:(DE-588)1065079516$$6P:(DE-2719)2811529$$aDeutsches Zentrum für Neurodegenerative Erkrankungen$$b1$$kDZNE
000140214 9101_ $$0I:(DE-588)1065079516$$6P:(DE-2719)2811502$$aDeutsches Zentrum für Neurodegenerative Erkrankungen$$b2$$kDZNE
000140214 9101_ $$0I:(DE-588)1065079516$$6P:(DE-2719)2810557$$aDeutsches Zentrum für Neurodegenerative Erkrankungen$$b3$$kDZNE
000140214 9101_ $$0I:(DE-588)1065079516$$6P:(DE-2719)2810376$$aDeutsches Zentrum für Neurodegenerative Erkrankungen$$b4$$kDZNE
000140214 9131_ $$0G:(DE-HGF)POF3-342$$1G:(DE-HGF)POF3-340$$2G:(DE-HGF)POF3-300$$3G:(DE-HGF)POF3$$4G:(DE-HGF)POF$$aDE-HGF$$bGesundheit$$lErkrankungen des Nervensystems$$vDisease Mechanisms and Model Systems$$x0
000140214 9132_ $$0G:(DE-HGF)POF4-899$$1G:(DE-HGF)POF4-890$$2G:(DE-HGF)POF4-800$$3G:(DE-HGF)POF4$$4G:(DE-HGF)POF$$aDE-HGF$$bProgrammungebundene Forschung$$lohne Programm$$vohne Topic$$x0
000140214 9141_ $$y2018
000140214 915__ $$0StatID:(DE-HGF)0200$$2StatID$$aDBCoverage$$bSCOPUS$$d2023-03-30
000140214 915__ $$0LIC:(DE-HGF)CCBYNV$$2V:(DE-HGF)$$aCreative Commons Attribution CC BY (No Version)$$bDOAJ
000140214 915__ $$0StatID:(DE-HGF)1050$$2StatID$$aDBCoverage$$bBIOSIS Previews$$d2023-03-30
000140214 915__ $$0StatID:(DE-HGF)0600$$2StatID$$aDBCoverage$$bEbsco Academic Search$$d2023-03-30
000140214 915__ $$0StatID:(DE-HGF)1040$$2StatID$$aDBCoverage$$bZoological Record$$d2023-03-30
000140214 915__ $$0StatID:(DE-HGF)0100$$2StatID$$aJCR$$bSCI REP-UK : 2021$$d2023-03-30
000140214 915__ $$0StatID:(DE-HGF)0501$$2StatID$$aDBCoverage$$bDOAJ Seal$$d2022-08-08T09:38:07Z
000140214 915__ $$0StatID:(DE-HGF)0500$$2StatID$$aDBCoverage$$bDOAJ$$d2022-08-08T09:38:07Z
000140214 915__ $$0StatID:(DE-HGF)0150$$2StatID$$aDBCoverage$$bWeb of Science Core Collection$$d2023-03-30
000140214 915__ $$0StatID:(DE-HGF)9900$$2StatID$$aIF < 5$$d2023-03-30
000140214 915__ $$0StatID:(DE-HGF)0510$$2StatID$$aOpenAccess
000140214 915__ $$0StatID:(DE-HGF)0030$$2StatID$$aPeer Review$$bASC$$d2023-03-30
000140214 915__ $$0StatID:(DE-HGF)1150$$2StatID$$aDBCoverage$$bCurrent Contents - Physical, Chemical and Earth Sciences$$d2023-03-30
000140214 915__ $$0StatID:(DE-HGF)0300$$2StatID$$aDBCoverage$$bMedline$$d2023-03-30
000140214 915__ $$0StatID:(DE-HGF)0199$$2StatID$$aDBCoverage$$bClarivate Analytics Master Journal List$$d2023-03-30
000140214 9201_ $$0I:(DE-2719)1013022$$kAG Tamgüney 2$$lPrion and prion-like diseases$$x0
000140214 980__ $$ajournal
000140214 980__ $$aVDB
000140214 980__ $$aUNRESTRICTED
000140214 980__ $$aI:(DE-2719)1013022
000140214 9801_ $$aFullTexts
000140214 999C5 $$2Crossref$$9-- missing cx lookup --$$a10.1101/cshperspect.a006833$$o10.1101/cshperspect.a006833
000140214 999C5 $$2Crossref$$9-- missing cx lookup --$$a10.1038/382180a0$$o10.1038/382180a0
000140214 999C5 $$2Crossref$$9-- missing cx lookup --$$a10.1016/j.jmb.2003.12.059$$o10.1016/j.jmb.2003.12.059
000140214 999C5 $$2Crossref$$9-- missing cx lookup --$$a10.1016/0092-8674(87)90150-4$$o10.1016/0092-8674(87)90150-4
000140214 999C5 $$2Crossref$$9-- missing cx lookup --$$a10.1021/bi00245a003$$o10.1021/bi00245a003
000140214 999C5 $$2Crossref$$9-- missing cx lookup --$$a10.1371/journal.ppat.1005835$$o10.1371/journal.ppat.1005835
000140214 999C5 $$2Crossref$$9-- missing cx lookup --$$a10.1371/journal.ppat.1004662$$o10.1371/journal.ppat.1004662
000140214 999C5 $$2Crossref$$9-- missing cx lookup --$$a10.1038/2654$$o10.1038/2654
000140214 999C5 $$2Crossref$$9-- missing cx lookup --$$a10.1098/rstb.2008.0069$$o10.1098/rstb.2008.0069
000140214 999C5 $$2Crossref$$9-- missing cx lookup --$$a10.1146/annurev.neuro.31.060407.125620$$o10.1146/annurev.neuro.31.060407.125620
000140214 999C5 $$2Crossref$$9-- missing cx lookup --$$a10.1016/j.bbadis.2007.05.001$$o10.1016/j.bbadis.2007.05.001
000140214 999C5 $$2Crossref$$9-- missing cx lookup --$$a10.1038/nn.2483$$o10.1038/nn.2483
000140214 999C5 $$2Crossref$$9-- missing cx lookup --$$a10.1038/nature19312$$o10.1038/nature19312
000140214 999C5 $$2Crossref$$9-- missing cx lookup --$$a10.1371/journal.pone.0007208$$o10.1371/journal.pone.0007208
000140214 999C5 $$2Crossref$$9-- missing cx lookup --$$a10.1371/journal.pone.0133741$$o10.1371/journal.pone.0133741
000140214 999C5 $$2Crossref$$9-- missing cx lookup --$$a10.1074/jbc.270.7.3299$$o10.1074/jbc.270.7.3299
000140214 999C5 $$2Crossref$$9-- missing cx lookup --$$a10.1074/jbc.M007114200$$o10.1074/jbc.M007114200
000140214 999C5 $$2Crossref$$9-- missing cx lookup --$$a10.1101/cshperspect.a024430$$o10.1101/cshperspect.a024430
000140214 999C5 $$2Crossref$$9-- missing cx lookup --$$a10.1038/35081095$$o10.1038/35081095
000140214 999C5 $$2Crossref$$9-- missing cx lookup --$$a10.1073/pnas.1834432100$$o10.1073/pnas.1834432100
000140214 999C5 $$2Crossref$$9-- missing cx lookup --$$a10.1371/journal.ppat.1001217$$o10.1371/journal.ppat.1001217
000140214 999C5 $$2Crossref$$9-- missing cx lookup --$$a10.1038/nmeth979$$o10.1038/nmeth979
000140214 999C5 $$2Crossref$$9-- missing cx lookup --$$a10.1073/pnas.061337998$$o10.1073/pnas.061337998
000140214 999C5 $$2Crossref$$9-- missing cx lookup --$$a10.1038/emboj.2008.122$$o10.1038/emboj.2008.122
000140214 999C5 $$2Crossref$$9-- missing cx lookup --$$a10.1126/science.289.5486.1925$$o10.1126/science.289.5486.1925
000140214 999C5 $$2Crossref$$9-- missing cx lookup --$$a10.1083/jcb.200409127$$o10.1083/jcb.200409127
000140214 999C5 $$2Crossref$$9-- missing cx lookup --$$a10.1006/jmbi.2000.5183$$o10.1006/jmbi.2000.5183
000140214 999C5 $$2Crossref$$9-- missing cx lookup --$$a10.1038/37783$$o10.1038/37783
000140214 999C5 $$2Crossref$$9-- missing cx lookup --$$a10.1021/ja077146j$$o10.1021/ja077146j
000140214 999C5 $$2Crossref$$9-- missing cx lookup --$$a10.1007/BF00143374$$o10.1007/BF00143374
000140214 999C5 $$2Crossref$$9-- missing cx lookup --$$a10.1073/pnas.93.25.14945$$o10.1073/pnas.93.25.14945
000140214 999C5 $$2Crossref$$9-- missing cx lookup --$$a10.1083/jcb.129.3.619$$o10.1083/jcb.129.3.619
000140214 999C5 $$2Crossref$$9-- missing cx lookup --$$a10.1007/s00018-004-4318-2$$o10.1007/s00018-004-4318-2
000140214 999C5 $$2Crossref$$9-- missing cx lookup --$$a10.1073/pnas.0710054104$$o10.1073/pnas.0710054104
000140214 999C5 $$2Crossref$$9-- missing cx lookup --$$a10.1128/JVI.74.9.4377-4386.2000$$o10.1128/JVI.74.9.4377-4386.2000
000140214 999C5 $$2Crossref$$9-- missing cx lookup --$$a10.1086/381166$$o10.1086/381166
000140214 999C5 $$2Crossref$$9-- missing cx lookup --$$a10.1128/JVI.74.1.320-325.2000$$o10.1128/JVI.74.1.320-325.2000
000140214 999C5 $$2Crossref$$9-- missing cx lookup --$$a10.1099/0022-1317-73-11-3027$$o10.1099/0022-1317-73-11-3027
000140214 999C5 $$2Crossref$$9-- missing cx lookup --$$a10.1099/0022-1317-39-3-487$$o10.1099/0022-1317-39-3-487
000140214 999C5 $$2Crossref$$9-- missing cx lookup --$$a10.1038/33834$$o10.1038/33834
000140214 999C5 $$2Crossref$$9-- missing cx lookup --$$a10.1128/JVI.75.21.10106-10112.2001$$o10.1128/JVI.75.21.10106-10112.2001
000140214 999C5 $$2Crossref$$9-- missing cx lookup --$$a10.1073/pnas.1317164110$$o10.1073/pnas.1317164110
000140214 999C5 $$2Crossref$$9-- missing cx lookup --$$a10.1517/17460441.2015.1016498$$o10.1517/17460441.2015.1016498
000140214 999C5 $$2Crossref$$9-- missing cx lookup --$$a10.1021/jm0002073$$o10.1021/jm0002073
000140214 999C5 $$2Crossref$$9-- missing cx lookup --$$a10.1038/sj.bjp.0704478$$o10.1038/sj.bjp.0704478
000140214 999C5 $$2Crossref$$9-- missing cx lookup --$$a10.1073/pnas.161274798$$o10.1073/pnas.161274798
000140214 999C5 $$2Crossref$$9-- missing cx lookup --$$a10.1128/JVI.74.10.4894-4897.2000$$o10.1128/JVI.74.10.4894-4897.2000
000140214 999C5 $$2Crossref$$9-- missing cx lookup --$$a10.1371/journal.pone.0039112$$o10.1371/journal.pone.0039112
000140214 999C5 $$2Crossref$$9-- missing cx lookup --$$a10.1038/nrd2572$$o10.1038/nrd2572
000140214 999C5 $$2Crossref$$9-- missing cx lookup --$$a10.1101/cshperspect.a023747$$o10.1101/cshperspect.a023747
000140214 999C5 $$2Crossref$$9-- missing cx lookup --$$a10.1074/jbc.M407006200$$o10.1074/jbc.M407006200
000140214 999C5 $$2Crossref$$9-- missing cx lookup --$$a10.1189/jlb.1106665$$o10.1189/jlb.1106665
000140214 999C5 $$2Crossref$$9-- missing cx lookup --$$a10.1111/j.1460-9568.2006.04805.x$$o10.1111/j.1460-9568.2006.04805.x
000140214 999C5 $$2Crossref$$9-- missing cx lookup --$$a10.1016/j.jmb.2014.10.024$$o10.1016/j.jmb.2014.10.024