%0 Journal Article
%A Blume, Jonas
%A Ziętara, Natalia
%A Witzlau, Katrin
%A Liu, Yanshan
%A Sanchez, Oskar Ortiz
%A Puchałka, Jacek
%A Winter, Samantha J
%A Kunze-Schumacher, Heike
%A Saran, Namita
%A Düber, Sandra
%A Roy, Bishnudeo
%A Weiss, Siegfried
%A Klein, Christoph
%A Wurst, Wolfgang
%A Łyszkiewicz, Marcin
%A Krueger, Andreas
%T miR-191 modulates B-cell development and targets transcription factors E2A, Foxp1, and Egr1.
%J European journal of immunology
%V 49
%N 1
%@ 0014-2980
%C Weinheim
%I Wiley-VCH
%M DZNE-2020-06734
%P 121-132
%D 2019
%X The interdependence of posttranscriptional gene regulation via miRNA and transcriptional regulatory networks in lymphocyte development is poorly understood. Here, we identified miR-191 as direct upstream modulator of a transcriptional module comprising the transcription factors Foxp1, E2A, and Egr1. Deletion as well as ectopic expression of miR-191 resulted in developmental arrest in B lineage cells, indicating that fine tuning of the combined expression levels of Foxp1, E2A, and Egr1, which in turn control somatic recombination and cytokine-driven expansion, constitutes a prerequisite for efficient B-cell development. In conclusion, we propose that miR-191 acts as a rheostat in B-cell development by fine tuning a key transcriptional program.
%K Animals
%K B-Lymphocytes: physiology
%K Basic Helix-Loop-Helix Transcription Factors: genetics
%K Basic Helix-Loop-Helix Transcription Factors: metabolism
%K Cell Differentiation
%K Cells, Cultured
%K Early Growth Response Protein 1: genetics
%K Early Growth Response Protein 1: metabolism
%K Forkhead Transcription Factors: genetics
%K Forkhead Transcription Factors: metabolism
%K Gene Regulatory Networks
%K Mice
%K Mice, Inbred C57BL
%K Mice, Knockout
%K MicroRNAs: genetics
%K RNA, Small Interfering: genetics
%K Recombination, Genetic
%K Repressor Proteins: genetics
%K Repressor Proteins: metabolism
%K Transcription, Genetic
%K Transgenes: genetics
%K Basic Helix-Loop-Helix Transcription Factors (NLM Chemicals)
%K Early Growth Response Protein 1 (NLM Chemicals)
%K Egr1 protein, mouse (NLM Chemicals)
%K Forkhead Transcription Factors (NLM Chemicals)
%K Foxp1 protein, mouse (NLM Chemicals)
%K MIRN191 microRNA, mouse (NLM Chemicals)
%K MicroRNAs (NLM Chemicals)
%K RNA, Small Interfering (NLM Chemicals)
%K Repressor Proteins (NLM Chemicals)
%K Tcf3 protein, mouse (NLM Chemicals)
%F PUB:(DE-HGF)16
%9 Journal Article
%$ pmid:30281154
%R 10.1002/eji.201847660
%U https://pub.dzne.de/record/140412