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| 001 | 140510 | ||
| 005 | 20250417133306.0 | ||
| 024 | 7 | _ | |a 10.1002/glia.23544 |2 doi |
| 024 | 7 | _ | |a pmid:30585358 |2 pmid |
| 024 | 7 | _ | |a 0894-1491 |2 ISSN |
| 024 | 7 | _ | |a 1098-1136 |2 ISSN |
| 024 | 7 | _ | |a altmetric:53162274 |2 altmetric |
| 037 | _ | _ | |a DZNE-2020-06832 |
| 041 | _ | _ | |a English |
| 082 | _ | _ | |a 610 |
| 100 | 1 | _ | |a Rakers, Cordula |0 P:(DE-2719)2810396 |b 0 |e First author |u dzne |
| 245 | _ | _ | |a Stroke target identification guided by astrocyte transcriptome analysis. |
| 260 | _ | _ | |a Bognor Regis [u.a.] |c 2019 |b Wiley-Liss |
| 264 | _ | 1 | |3 online |2 Crossref |b Wiley |c 2018-12-26 |
| 264 | _ | 1 | |3 print |2 Crossref |b Wiley |c 2019-04-01 |
| 336 | 7 | _ | |a article |2 DRIVER |
| 336 | 7 | _ | |a Output Types/Journal article |2 DataCite |
| 336 | 7 | _ | |a Journal Article |b journal |m journal |0 PUB:(DE-HGF)16 |s 1744889540_11896 |2 PUB:(DE-HGF) |
| 336 | 7 | _ | |a ARTICLE |2 BibTeX |
| 336 | 7 | _ | |a JOURNAL_ARTICLE |2 ORCID |
| 336 | 7 | _ | |a Journal Article |0 0 |2 EndNote |
| 520 | _ | _ | |a Astrocytes support normal brain function, but may also contribute to neurodegeneration when they become reactive under pathological conditions such as stroke. However, the molecular underpinnings of this context-dependent interplay between beneficial and detrimental properties in reactive astrogliosis have remained incompletely understood. Therefore, using the RiboTag technique, we immunopurified translating mRNAs specifically from astrocytes 72 hr after transient middle cerebral artery occlusion in mice (tMCAO), thereby generating a stroke-specific astroglial translatome database. We found that compared to control brains, reactive astrocytes after tMCAO show an enrichment of transcripts linked to the A2 phenotype, which has been associated with neuroprotection. However, we found that astrocytes also upregulate a large number of potentially neurotoxic genes. In total, we identified the differential expression of 1,003 genes and 38 transcription factors, of which Stat3, Sp1, and Spi1 were the most prominent. To further explore the effects of Stat3-mediated pathways on stroke pathogenesis, we subjected mice with an astrocyte-specific conditional deletion of Stat3 to tMCAO, and found that these mice have reduced stroke volume and improved motor outcome 72 hr after focal ischemia. Taken together, our study extends the emerging database of novel astrocyte-specific targets for stroke therapy, and supports the role of astrocytes as critical safeguards of brain function in health and disease. |
| 536 | _ | _ | |a 342 - Disease Mechanisms and Model Systems (POF3-342) |0 G:(DE-HGF)POF3-342 |c POF3-342 |f POF III |x 0 |
| 536 | _ | _ | |a 344 - Clinical and Health Care Research (POF3-344) |0 G:(DE-HGF)POF3-344 |c POF3-344 |f POF III |x 1 |
| 542 | _ | _ | |i 2018-12-26 |2 Crossref |u http://doi.wiley.com/10.1002/tdm_license_1.1 |
| 542 | _ | _ | |i 2018-12-26 |2 Crossref |u http://onlinelibrary.wiley.com/termsAndConditions#vor |
| 588 | _ | _ | |a Dataset connected to CrossRef, PubMed, |
| 650 | _ | 7 | |a Connexin 43 |2 NLM Chemicals |
| 650 | _ | 7 | |a Galectin 3 |2 NLM Chemicals |
| 650 | _ | 7 | |a Lipocalin-2 |2 NLM Chemicals |
| 650 | _ | 7 | |a Luminescent Proteins |2 NLM Chemicals |
| 650 | _ | 7 | |a Nerve Tissue Proteins |2 NLM Chemicals |
| 650 | _ | 7 | |a STAT3 Transcription Factor |2 NLM Chemicals |
| 650 | _ | 7 | |a Stat3 protein, mouse |2 NLM Chemicals |
| 650 | _ | 2 | |a Animals |2 MeSH |
| 650 | _ | 2 | |a Astrocytes: metabolism |2 MeSH |
| 650 | _ | 2 | |a Computational Biology |2 MeSH |
| 650 | _ | 2 | |a Connexin 43: genetics |2 MeSH |
| 650 | _ | 2 | |a Connexin 43: metabolism |2 MeSH |
| 650 | _ | 2 | |a Disease Models, Animal |2 MeSH |
| 650 | _ | 2 | |a Female |2 MeSH |
| 650 | _ | 2 | |a Galectin 3: genetics |2 MeSH |
| 650 | _ | 2 | |a Galectin 3: metabolism |2 MeSH |
| 650 | _ | 2 | |a Gene Expression Profiling: methods |2 MeSH |
| 650 | _ | 2 | |a Gene Expression Regulation: genetics |2 MeSH |
| 650 | _ | 2 | |a Immunoprecipitation |2 MeSH |
| 650 | _ | 2 | |a Infarction, Middle Cerebral Artery: pathology |2 MeSH |
| 650 | _ | 2 | |a Infarction, Middle Cerebral Artery: physiopathology |2 MeSH |
| 650 | _ | 2 | |a Lipocalin-2: genetics |2 MeSH |
| 650 | _ | 2 | |a Lipocalin-2: metabolism |2 MeSH |
| 650 | _ | 2 | |a Luminescent Proteins: genetics |2 MeSH |
| 650 | _ | 2 | |a Luminescent Proteins: metabolism |2 MeSH |
| 650 | _ | 2 | |a Male |2 MeSH |
| 650 | _ | 2 | |a Mice |2 MeSH |
| 650 | _ | 2 | |a Mice, Inbred C57BL |2 MeSH |
| 650 | _ | 2 | |a Mice, Transgenic |2 MeSH |
| 650 | _ | 2 | |a Nerve Tissue Proteins: metabolism |2 MeSH |
| 650 | _ | 2 | |a Rhombencephalon: pathology |2 MeSH |
| 650 | _ | 2 | |a Rotarod Performance Test |2 MeSH |
| 650 | _ | 2 | |a STAT3 Transcription Factor: genetics |2 MeSH |
| 650 | _ | 2 | |a STAT3 Transcription Factor: metabolism |2 MeSH |
| 700 | 1 | _ | |a Schleif, Melvin |0 P:(DE-2719)2810344 |b 1 |u dzne |
| 700 | 1 | _ | |a Blank, Nelli |0 P:(DE-2719)2811722 |b 2 |u dzne |
| 700 | 1 | _ | |a Matušková, Hana |0 P:(DE-2719)9000859 |b 3 |u dzne |
| 700 | 1 | _ | |a Ulas, Thomas |0 P:(DE-HGF)0 |b 4 |
| 700 | 1 | _ | |a Händler, Kristian |0 P:(DE-HGF)0 |b 5 |
| 700 | 1 | _ | |a Torres, Santiago Valle |b 6 |
| 700 | 1 | _ | |a Schumacher, Toni |0 P:(DE-2719)2810699 |b 7 |u dzne |
| 700 | 1 | _ | |a Tai, Khalid |0 P:(DE-2719)2811148 |b 8 |u dzne |
| 700 | 1 | _ | |a Schultze, Joachim L |0 P:(DE-2719)2811660 |b 9 |u dzne |
| 700 | 1 | _ | |a Jackson, Walker S |0 P:(DE-2719)2810253 |b 10 |u dzne |
| 700 | 1 | _ | |a Petzold, Gabor Claus Julius Peter |0 P:(DE-2719)2810273 |b 11 |e Last author |u dzne |
| 773 | 1 | 8 | |a 10.1002/glia.23544 |b : Wiley, 2018-12-26 |n 4 |p 619-633 |3 journal-article |2 Crossref |t Glia |v 67 |y 2018 |x 0894-1491 |
| 773 | _ | _ | |a 10.1002/glia.23544 |g Vol. 67, no. 4, p. 619 - 633 |0 PERI:(DE-600)1474828-9 |n 4 |q 67:4<619 - 633 |p 619-633 |t Glia |v 67 |y 2019 |x 0894-1491 |
| 856 | 4 | _ | |u https://pub.dzne.de/record/140510/files/DZNE-2020-06832_Restricted.pdf |
| 856 | 4 | _ | |u https://pub.dzne.de/record/140510/files/DZNE-2020-06832_Restricted.pdf?subformat=pdfa |x pdfa |
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| 913 | 1 | _ | |a DE-HGF |b Gesundheit |l Erkrankungen des Nervensystems |1 G:(DE-HGF)POF3-340 |0 G:(DE-HGF)POF3-342 |3 G:(DE-HGF)POF3 |2 G:(DE-HGF)POF3-300 |4 G:(DE-HGF)POF |v Disease Mechanisms and Model Systems |x 0 |
| 913 | 1 | _ | |a DE-HGF |b Gesundheit |l Erkrankungen des Nervensystems |1 G:(DE-HGF)POF3-340 |0 G:(DE-HGF)POF3-344 |3 G:(DE-HGF)POF3 |2 G:(DE-HGF)POF3-300 |4 G:(DE-HGF)POF |v Clinical and Health Care Research |x 1 |
| 914 | 1 | _ | |y 2019 |
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| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)1050 |2 StatID |b BIOSIS Previews |d 2022-11-08 |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)1030 |2 StatID |b Current Contents - Life Sciences |d 2022-11-08 |
| 915 | _ | _ | |a JCR |0 StatID:(DE-HGF)0100 |2 StatID |b GLIA : 2021 |d 2022-11-08 |
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| 920 | 1 | _ | |0 I:(DE-2719)1013020 |k AG Petzold |l Vascular Neurology |x 0 |
| 920 | 1 | _ | |0 I:(DE-2719)1013019 |k AG Jackson |l Selective Vulnerability of Neurodegenerative Diseases |x 1 |
| 920 | 1 | _ | |0 I:(DE-2719)1011006 |k AG Krauß |l Regulatory RNA-protein interaction in neurodegenerative diseases |x 2 |
| 920 | 1 | _ | |0 I:(DE-2719)1013038 |k AG Schultze |l Clinical Single Cell Omics (CSCO) / Systems Medicine |x 3 |
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