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@ARTICLE{Niemeyer:140604,
      author       = {Niemeyer, Johannes and Mentrup, Torben and Heidasch, Ronny
                      and Müller, Stephan A and Biswas, Uddipta and Meyer, Rieke
                      and Papadopoulou, Alkmini A and Dederer, Verena and
                      Haug-Kröper, Martina and Adamski, Vivian and
                      Lüllmann-Rauch, Renate and Bergmann, Martin and Mayerhofer,
                      Artur and Saftig, Paul and Wennemuth, Gunther and
                      Jessberger, Rolf and Fluhrer, Regina and Lichtenthaler,
                      Stefan F and Lemberg, Marius K and Schröder, Bernd},
      title        = {{T}he intramembrane protease {SPPL}2c promotes male germ
                      cell development by cleaving phospholamban.},
      journal      = {EMBO reports},
      volume       = {20},
      number       = {3},
      issn         = {1469-221X},
      address      = {Hoboken, NJ [u.a.]},
      publisher    = {Wiley},
      reportid     = {DZNE-2020-06926},
      pages        = {e46449},
      year         = {2019},
      abstract     = {Signal peptide peptidase (SPP) and the four homologous
                      SPP-like (SPPL) proteases constitute a family of
                      intramembrane aspartyl proteases with selectivity for type
                      II-oriented transmembrane segments. Here, we analyse the
                      physiological function of the orphan protease SPPL2c,
                      previously considered to represent a non-expressed
                      pseudogene. We demonstrate proteolytic activity of SPPL2c
                      towards selected tail-anchored proteins. Despite shared ER
                      localisation, SPPL2c and SPP exhibit distinct, though
                      partially overlapping substrate spectra and inhibitory
                      profiles, and are organised in different high molecular
                      weight complexes. Interestingly, SPPL2c is specifically
                      expressed in murine and human testis where it is primarily
                      localised in spermatids. In mice, SPPL2c deficiency leads to
                      a partial loss of elongated spermatids and reduced motility
                      of mature spermatozoa, but preserved fertility. However,
                      matings of male and female SPPL2c-/- mice exhibit reduced
                      litter sizes. Using proteomics we identify the
                      sarco/endoplasmic reticulum Ca2+-ATPase (SERCA2)-regulating
                      protein phospholamban (PLN) as a physiological SPPL2c
                      substrate. Accumulation of PLN correlates with a decrease in
                      intracellular Ca2+ levels in elongated spermatids that
                      likely contribute to the compromised male germ cell
                      differentiation and function of SPPL2c-/- mice.},
      keywords     = {Amino Acid Sequence / Animals / Aspartic Acid
                      Endopeptidases: chemistry / Aspartic Acid Endopeptidases:
                      metabolism / Calcium: metabolism / Calcium-Binding Proteins:
                      metabolism / Cell Membrane: enzymology / Endoplasmic
                      Reticulum: metabolism / Female / Germ Cells: metabolism /
                      HEK293 Cells / HeLa Cells / Homeostasis / Humans / Male /
                      Membrane Proteins: chemistry / Membrane Proteins: metabolism
                      / Mice / Organ Specificity / Spermatids: metabolism /
                      Substrate Specificity / Testis: enzymology},
      cin          = {AG Haass / AG Fluhrer / AG Lichtenthaler},
      ddc          = {570},
      cid          = {I:(DE-2719)1110007 / I:(DE-2719)1110000-2 /
                      I:(DE-2719)1110006},
      pnm          = {342 - Disease Mechanisms and Model Systems (POF3-342)},
      pid          = {G:(DE-HGF)POF3-342},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:30733280},
      pmc          = {pmc:PMC6399600},
      doi          = {10.15252/embr.201846449},
      url          = {https://pub.dzne.de/record/140604},
}