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@ARTICLE{Papadopoulou:140605,
author = {Papadopoulou, Alkmini A and Müller, Stephan A and Mentrup,
Torben and Shmueli, Merav D and Niemeyer, Johannes and
Haug-Kröper, Martina and von Blume, Julia and Mayerhofer,
Artur and Feederle, Regina and Schröder, Bernd and
Lichtenthaler, Stefan F and Fluhrer, Regina},
title = {{S}ignal {P}eptide {P}eptidase-{L}ike 2c ({SPPL}2c) impairs
vesicular transport and cleaves {SNARE} proteins.},
journal = {EMBO reports},
volume = {20},
number = {3},
issn = {1469-221X},
address = {Hoboken, NJ [u.a.]},
publisher = {Wiley},
reportid = {DZNE-2020-06927},
pages = {e46451},
year = {2019},
note = {Title corrected according to Corrigendum: EMBO rep (2019)
20: e48133 https://doi.org/10.15252/embr.201948133},
abstract = {Members of the GxGD-type intramembrane aspartyl proteases
have emerged as key players not only in fundamental cellular
processes such as B-cell development or protein
glycosylation, but also in development of pathologies, such
as Alzheimer's disease or hepatitis virus infections.
However, one member of this protease family, signal peptide
peptidase-like 2c (SPPL2c), remains orphan and its
capability of proteolysis as well as its physiological
function is still enigmatic. Here, we demonstrate that
SPPL2c is catalytically active and identify a variety of
SPPL2c candidate substrates using proteomics. The majority
of the SPPL2c candidate substrates cluster to the biological
process of vesicular trafficking. Analysis of selected SNARE
proteins reveals proteolytic processing by SPPL2c that
impairs vesicular transport and causes retention of cargo
proteins in the endoplasmic reticulum. As a consequence, the
integrity of subcellular compartments, in particular the
Golgi, is disturbed. Together with a strikingly high
physiological SPPL2c expression in testis, our data suggest
involvement of SPPL2c in acrosome formation during
spermatogenesis.},
cin = {AG Haass / AG Lichtenthaler / AG Feederle / AG Fluhrer},
ddc = {570},
cid = {I:(DE-2719)1110007 / I:(DE-2719)1110006 /
I:(DE-2719)1140004 / I:(DE-2719)1110000-2},
pnm = {342 - Disease Mechanisms and Model Systems (POF3-342)},
pid = {G:(DE-HGF)POF3-342},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:30733281},
pmc = {pmc:PMC6399617},
doi = {10.15252/embr.201846451},
url = {https://pub.dzne.de/record/140605},
}