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@ARTICLE{Brgers:140636,
author = {Bürgers, Jana and Pavlova, Irina and Rodriguez-Gatica,
Juan E and Henneberger, Christian and Oeller, Marc and
Ruland, Jan A and Siebrasse, Jan P and Kubitscheck, Ulrich
and Schwarz, Martin K},
title = {{L}ight-sheet fluorescence expansion microscopy: fast
mapping of neural circuits at super resolution.},
journal = {Neurophotonics},
volume = {6},
number = {01},
issn = {2329-423X},
address = {Bellingham, Wash.},
publisher = {SPIE},
reportid = {DZNE-2020-06958},
pages = {1},
year = {2019},
abstract = {The goal of understanding the architecture of neural
circuits at the synapse level with a brain-wide perspective
has powered the interest in high-speed and large
field-of-view volumetric imaging at subcellular resolution.
Here, we developed a method combining tissue expansion and
light-sheet fluorescence microscopy to allow extended
volumetric super resolution high-speed imaging of large
mouse brain samples. We demonstrate the capabilities of this
method by performing two color fast volumetric super
resolution imaging of mouse CA1 and dentate gyrus
molecular-, granule cell-, and polymorphic layers. Our
method enables an exact evaluation of granule cell and
neurite morphology within the context of large cell
ensembles spanning several orders of magnitude in
resolution. We found that imaging a brain region of 1
mm 3 in super resolution using light-sheet fluorescence
expansion microscopy is about 17-fold faster than imaging
the same region by a current state-of-the-art
high-resolution confocal laser scanning microscope.},
cin = {U Preclinical Researchers - Bonn},
cid = {I:(DE-2719)7000005},
pnm = {342 - Disease Mechanisms and Model Systems (POF3-342)},
pid = {G:(DE-HGF)POF3-342},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:30796881},
pmc = {pmc:PMC6368534},
doi = {10.1117/1.NPh.6.1.015005},
url = {https://pub.dzne.de/record/140636},
}