guest ::
| Home > Publications Database > Light-sheet fluorescence expansion microscopy: fast mapping of neural circuits at super resolution. > print |
| Home > Publications Database > Light-sheet fluorescence expansion microscopy: fast mapping of neural circuits at super resolution. > print |
| 001 | 140636 | ||
| 005 | 20240430120009.0 | ||
| 024 | 7 | _ | |a 10.1117/1.NPh.6.1.015005 |2 doi |
| 024 | 7 | _ | |a pmid:30796881 |2 pmid |
| 024 | 7 | _ | |a pmc:PMC6368534 |2 pmc |
| 024 | 7 | _ | |a 2329-423X |2 ISSN |
| 024 | 7 | _ | |a 2329-4248 |2 ISSN |
| 024 | 7 | _ | |a altmetric:55989743 |2 altmetric |
| 037 | _ | _ | |a DZNE-2020-06958 |
| 041 | _ | _ | |a English |
| 100 | 1 | _ | |a Bürgers, Jana |b 0 |
| 245 | _ | _ | |a Light-sheet fluorescence expansion microscopy: fast mapping of neural circuits at super resolution. |
| 260 | _ | _ | |a Bellingham, Wash. |c 2019 |b SPIE |
| 264 | _ | 1 | |3 print |2 Crossref |b SPIE-Intl Soc Optical Eng |c 2019-02-08 |
| 336 | 7 | _ | |a article |2 DRIVER |
| 336 | 7 | _ | |a Output Types/Journal article |2 DataCite |
| 336 | 7 | _ | |a Journal Article |b journal |m journal |0 PUB:(DE-HGF)16 |s 1714467044_8224 |2 PUB:(DE-HGF) |
| 336 | 7 | _ | |a ARTICLE |2 BibTeX |
| 336 | 7 | _ | |a JOURNAL_ARTICLE |2 ORCID |
| 336 | 7 | _ | |a Journal Article |0 0 |2 EndNote |
| 520 | _ | _ | |a The goal of understanding the architecture of neural circuits at the synapse level with a brain-wide perspective has powered the interest in high-speed and large field-of-view volumetric imaging at subcellular resolution. Here, we developed a method combining tissue expansion and light-sheet fluorescence microscopy to allow extended volumetric super resolution high-speed imaging of large mouse brain samples. We demonstrate the capabilities of this method by performing two color fast volumetric super resolution imaging of mouse CA1 and dentate gyrus molecular-, granule cell-, and polymorphic layers. Our method enables an exact evaluation of granule cell and neurite morphology within the context of large cell ensembles spanning several orders of magnitude in resolution. We found that imaging a brain region of 1 mm 3 in super resolution using light-sheet fluorescence expansion microscopy is about 17-fold faster than imaging the same region by a current state-of-the-art high-resolution confocal laser scanning microscope. |
| 536 | _ | _ | |a 342 - Disease Mechanisms and Model Systems (POF3-342) |0 G:(DE-HGF)POF3-342 |c POF3-342 |f POF III |x 0 |
| 588 | _ | _ | |a Dataset connected to CrossRef, PubMed, |
| 700 | 1 | _ | |a Pavlova, Irina |0 P:(DE-HGF)0 |b 1 |
| 700 | 1 | _ | |a Rodriguez-Gatica, Juan E |b 2 |
| 700 | 1 | _ | |a Henneberger, Christian |0 P:(DE-2719)2811625 |b 3 |u dzne |
| 700 | 1 | _ | |a Oeller, Marc |0 P:(DE-HGF)0 |b 4 |
| 700 | 1 | _ | |a Ruland, Jan A |b 5 |
| 700 | 1 | _ | |a Siebrasse, Jan P |b 6 |
| 700 | 1 | _ | |a Kubitscheck, Ulrich |0 P:(DE-HGF)0 |b 7 |e Corresponding author |
| 700 | 1 | _ | |a Schwarz, Martin K |b 8 |
| 773 | 1 | 8 | |a 10.1117/1.nph.6.1.015005 |b : SPIE-Intl Soc Optical Eng, 2019-02-08 |n 01 |p 1 |3 journal-article |2 Crossref |t Neurophotonics |v 6 |y 2019 |x 2329-423X |
| 773 | _ | _ | |a 10.1117/1.NPh.6.1.015005 |g Vol. 6, no. 1, p. 1 - |0 PERI:(DE-600)2781943-7 |n 01 |q 6:1<1 - |p 1 |t Neurophotonics |v 6 |y 2019 |x 2329-423X |
| 856 | 4 | _ | |y OpenAccess |u https://pub.dzne.de/record/140636/files/DZNE-2020-06958.pdf |
| 856 | 4 | _ | |y OpenAccess |x pdfa |u https://pub.dzne.de/record/140636/files/DZNE-2020-06958.pdf?subformat=pdfa |
| 856 | 7 | _ | |2 Pubmed Central |u http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6368534 |
| 909 | C | O | |o oai:pub.dzne.de:140636 |p openaire |p open_access |p VDB |p driver |p dnbdelivery |
| 910 | 1 | _ | |a Deutsches Zentrum für Neurodegenerative Erkrankungen |0 I:(DE-588)1065079516 |k DZNE |b 3 |6 P:(DE-2719)2811625 |
| 913 | 1 | _ | |a DE-HGF |b Gesundheit |l Erkrankungen des Nervensystems |1 G:(DE-HGF)POF3-340 |0 G:(DE-HGF)POF3-342 |3 G:(DE-HGF)POF3 |2 G:(DE-HGF)POF3-300 |4 G:(DE-HGF)POF |v Disease Mechanisms and Model Systems |x 0 |
| 914 | 1 | _ | |y 2019 |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)0200 |2 StatID |b SCOPUS |d 2022-11-25 |
| 915 | _ | _ | |a Creative Commons Attribution CC BY 4.0 |0 LIC:(DE-HGF)CCBY4 |2 HGFVOC |
| 915 | _ | _ | |a JCR |0 StatID:(DE-HGF)0100 |2 StatID |b NEUROPHOTONICS : 2021 |d 2022-11-25 |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)0501 |2 StatID |b DOAJ Seal |d 2019-07-11T08:15:40Z |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)0500 |2 StatID |b DOAJ |d 2019-07-11T08:15:40Z |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)0150 |2 StatID |b Web of Science Core Collection |d 2022-11-25 |
| 915 | _ | _ | |a IF < 5 |0 StatID:(DE-HGF)9900 |2 StatID |d 2022-11-25 |
| 915 | _ | _ | |a OpenAccess |0 StatID:(DE-HGF)0510 |2 StatID |
| 915 | _ | _ | |a Peer Review |0 StatID:(DE-HGF)0030 |2 StatID |b DOAJ : Blind peer review |d 2019-07-11T08:15:40Z |
| 915 | _ | _ | |a National-Konsortium |0 StatID:(DE-HGF)0430 |2 StatID |d 2022-11-25 |w ger |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)0300 |2 StatID |b Medline |d 2022-11-25 |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)0199 |2 StatID |b Clarivate Analytics Master Journal List |d 2022-11-25 |
| 920 | 1 | _ | |0 I:(DE-2719)7000005 |k U Preclinical Researchers - Bonn |l U Preclinical Researchers - Bonn |x 0 |
| 980 | _ | _ | |a journal |
| 980 | _ | _ | |a VDB |
| 980 | _ | _ | |a UNRESTRICTED |
| 980 | _ | _ | |a I:(DE-2719)7000005 |
| 980 | 1 | _ | |a FullTexts |
| 999 | C | 5 | |y 1899–1904 |2 Crossref |o Ramón 1899–1904 |
| 999 | C | 5 | |9 -- missing cx lookup -- |a 10.1038/nmeth.3361 |2 Crossref |o 10.1038/nmeth.3361 |
| 999 | C | 5 | |9 -- missing cx lookup -- |a 10.1038/nmeth.2477 |2 Crossref |o 10.1038/nmeth.2477 |
| 999 | C | 5 | |9 -- missing cx lookup -- |a 10.1038/nmeth815 |2 Crossref |o 10.1038/nmeth815 |
| 999 | C | 5 | |9 -- missing cx lookup -- |a 10.1038/nmeth818 |2 Crossref |o 10.1038/nmeth818 |
| 999 | C | 5 | |9 -- missing cx lookup -- |a 10.1073/pnas.1119590109 |2 Crossref |o 10.1073/pnas.1119590109 |
| 999 | C | 5 | |9 -- missing cx lookup -- |a 10.1016/j.neuron.2010.09.024 |2 Crossref |o 10.1016/j.neuron.2010.09.024 |
| 999 | C | 5 | |9 -- missing cx lookup -- |a 10.1038/nmeth.1854 |2 Crossref |o 10.1038/nmeth.1854 |
| 999 | C | 5 | |9 -- missing cx lookup -- |a 10.1002/lpor.2013.7.issue-5 |2 Crossref |o 10.1002/lpor.2013.7.issue-5 |
| 999 | C | 5 | |9 -- missing cx lookup -- |a 10.1016/j.cell.2015.06.067 |2 Crossref |o 10.1016/j.cell.2015.06.067 |
| 999 | C | 5 | |9 -- missing cx lookup -- |a 10.1146/annurev-cellbio-111315-125001 |2 Crossref |o 10.1146/annurev-cellbio-111315-125001 |
| 999 | C | 5 | |9 -- missing cx lookup -- |a 10.1186/s13041-017-0314-y |2 Crossref |o 10.1186/s13041-017-0314-y |
| 999 | C | 5 | |y 2013 |2 Crossref |o Kim 2013 |
| 999 | C | 5 | |9 -- missing cx lookup -- |a 10.1038/nature12107 |2 Crossref |o 10.1038/nature12107 |
| 999 | C | 5 | |9 -- missing cx lookup -- |a 10.1126/science.1260088 |2 Crossref |o 10.1126/science.1260088 |
| 999 | C | 5 | |9 -- missing cx lookup -- |a 10.1038/nmeth.3833 |2 Crossref |o 10.1038/nmeth.3833 |
| 999 | C | 5 | |9 -- missing cx lookup -- |a 10.1038/nmeth.4261 |2 Crossref |o 10.1038/nmeth.4261 |
| 999 | C | 5 | |9 -- missing cx lookup -- |a 10.1126/science.aaa5084 |2 Crossref |o 10.1126/science.aaa5084 |
| 999 | C | 5 | |9 -- missing cx lookup -- |a 10.1038/nmeth1036 |2 Crossref |o 10.1038/nmeth1036 |
| 999 | C | 5 | |9 -- missing cx lookup -- |a 10.1364/OE.20.020582 |2 Crossref |o 10.1364/OE.20.020582 |
| 999 | C | 5 | |9 -- missing cx lookup -- |a 10.1371/journal.pone.0124650 |2 Crossref |o 10.1371/journal.pone.0124650 |
| 999 | C | 5 | |9 -- missing cx lookup -- |a 10.1038/ncomms14162 |2 Crossref |o 10.1038/ncomms14162 |
| 999 | C | 5 | |9 -- missing cx lookup -- |a 10.1111/jmi.1993.170.issue-3 |2 Crossref |o 10.1111/jmi.1993.170.issue-3 |
| 999 | C | 5 | |9 -- missing cx lookup -- |a 10.1126/science.1100035 |2 Crossref |o 10.1126/science.1100035 |
| 999 | C | 5 | |9 -- missing cx lookup -- |a 10.1038/nmeth.3219 |2 Crossref |o 10.1038/nmeth.3219 |
| 999 | C | 5 | |9 -- missing cx lookup -- |a 10.1364/OE.20.021805 |2 Crossref |o 10.1364/OE.20.021805 |
| 999 | C | 5 | |9 -- missing cx lookup -- |a 10.3390/photonics4030041 |2 Crossref |o 10.3390/photonics4030041 |
| 999 | C | 5 | |9 -- missing cx lookup -- |a 10.1093/bioinformatics/btp184 |2 Crossref |o 10.1093/bioinformatics/btp184 |
| 999 | C | 5 | |9 -- missing cx lookup -- |a 10.1038/nmeth.4224 |2 Crossref |o 10.1038/nmeth.4224 |
| 999 | C | 5 | |9 -- missing cx lookup -- |a 10.1146/annurev-cellbio-100109-104048 |2 Crossref |o 10.1146/annurev-cellbio-100109-104048 |
| 999 | C | 5 | |9 -- missing cx lookup -- |a 10.1111/ejn.2018.47.issue-9 |2 Crossref |o 10.1111/ejn.2018.47.issue-9 |
| 999 | C | 5 | |9 -- missing cx lookup -- |a 10.1038/nbt.3625 |2 Crossref |o 10.1038/nbt.3625 |
| 999 | C | 5 | |9 -- missing cx lookup -- |a 10.1016/j.conb.2011.08.003 |2 Crossref |o 10.1016/j.conb.2011.08.003 |
| 999 | C | 5 | |9 -- missing cx lookup -- |a 10.1007/s12264-016-0053-6 |2 Crossref |o 10.1007/s12264-016-0053-6 |
| 999 | C | 5 | |y 2014 |2 Crossref |o Chen 2014 |
| 999 | C | 5 | |9 -- missing cx lookup -- |a 10.1523/JNEUROSCI.2707-07.2007 |2 Crossref |o 10.1523/JNEUROSCI.2707-07.2007 |
| Library | Collection | CLSMajor | CLSMinor | Language | Author |
|---|