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@ARTICLE{vanWell:140707,
author = {van Well, Eva M and Bader, Verian and Patra, Maria and
Sánchez-Vicente, Ana and Meschede, Jens and Furthmann,
Nikolas and Schnack, Cathrin and Blusch, Alina and
Longworth, Joseph and Petrasch-Parwez, Elisabeth and Mori,
Kohji and Arzberger, Thomas and Trümbach, Dietrich and
Angersbach, Lena and Showkat, Cathrin and Sehr, Dominik A
and Berlemann, Lena A and Goldmann, Petra and Clement,
Albrecht M and Behl, Christian and Woerner, Andreas C and
Saft, Carsten and Wurst, Wolfgang and Haass, Christian and
Ellrichmann, Gisa and Gold, Ralf and Dittmar, Gunnar and
Hipp, Mark S and Hartl, F Ulrich and Tatzelt, Jörg and
Winklhofer, Konstanze F},
title = {{A} protein quality control pathway regulated by linear
ubiquitination.},
journal = {The EMBO journal},
volume = {38},
number = {9},
issn = {0261-4189},
address = {Hoboken, NJ [u.a.]},
publisher = {Wiley},
reportid = {DZNE-2020-07029},
pages = {e100730},
year = {2019},
abstract = {Neurodegenerative diseases are characterized by the
accumulation of misfolded proteins in the brain. Insights
into protein quality control mechanisms to prevent neuronal
dysfunction and cell death are crucial in developing causal
therapies. Here, we report that various disease-associated
protein aggregates are modified by the linear ubiquitin
chain assembly complex (LUBAC). HOIP, the catalytic
component of LUBAC, is recruited to misfolded Huntingtin in
a p97/VCP-dependent manner, resulting in the assembly of
linear polyubiquitin. As a consequence, the interactive
surface of misfolded Huntingtin species is shielded from
unwanted interactions, for example with the low complexity
sequence domain-containing transcription factor Sp1, and
proteasomal degradation of misfolded Huntingtin is
facilitated. Notably, all three core LUBAC components are
transcriptionally regulated by Sp1, linking defective LUBAC
expression to Huntington's disease. In support of a
protective activity of linear ubiquitination, silencing of
OTULIN, a deubiquitinase with unique specificity for linear
polyubiquitin, decreases proteotoxicity, whereas silencing
of HOIP has the opposite effect. These findings identify
linear ubiquitination as a protein quality control mechanism
and hence a novel target for disease-modifying strategies in
proteinopathies.},
keywords = {Adult / Aged / Animals / Brain: metabolism / Brain:
pathology / Case-Control Studies / Cells, Cultured / Embryo,
Mammalian: cytology / Embryo, Mammalian: metabolism / Female
/ Fibroblasts: cytology / Fibroblasts: metabolism / Humans /
Huntingtin Protein: genetics / Huntingtin Protein:
metabolism / Huntington Disease: genetics / Huntington
Disease: metabolism / Huntington Disease: pathology / Male /
Mice / Mice, Knockout / Middle Aged / NF-kappa B: genetics /
NF-kappa B: metabolism / Neurons: metabolism / Neurons:
pathology / Polyubiquitin: metabolism / Protein Binding /
Protein Interaction Domains and Motifs / Protein Processing,
Post-Translational / Signal Transduction / Sp1 Transcription
Factor: genetics / Sp1 Transcription Factor: metabolism /
Ubiquitination / Valosin Containing Protein: genetics /
Valosin Containing Protein: metabolism / HTT protein, human
(NLM Chemicals) / Huntingtin Protein (NLM Chemicals) /
NF-kappa B (NLM Chemicals) / Sp1 Transcription Factor (NLM
Chemicals) / Sp1 protein, human (NLM Chemicals) /
Polyubiquitin (NLM Chemicals) / Valosin Containing Protein
(NLM Chemicals)},
cin = {AG Levin / AG Wurst / AG Haass / AG Winklhofer},
ddc = {570},
cid = {I:(DE-2719)1111016 / I:(DE-2719)1140001 /
I:(DE-2719)1110007 / I:(DE-2719)5000047},
pnm = {342 - Disease Mechanisms and Model Systems (POF3-342) / 344
- Clinical and Health Care Research (POF3-344) / 341 -
Molecular Signaling (POF3-341)},
pid = {G:(DE-HGF)POF3-342 / G:(DE-HGF)POF3-344 /
G:(DE-HGF)POF3-341},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:30886048},
pmc = {pmc:PMC6484417},
doi = {10.15252/embj.2018100730},
url = {https://pub.dzne.de/record/140707},
}