%0 Journal Article
%A Weise, Stefan C
%A Arumugam, Ganeshkumar
%A Villarreal, Alejandro
%A Videm, Pavankumar
%A Heidrich, Stefanie
%A Nebel, Nils
%A Dumit, Verónica I
%A Sananbenesi, Farahnaz
%A Reimann, Viktoria
%A Craske, Madeline
%A Schilling, Oliver
%A Hess, Wolfgang R
%A Fischer, Andre
%A Backofen, Rolf
%A Vogel, Tanja
%T FOXG1 Regulates PRKAR2B Transcriptionally and Posttranscriptionally via miR200 in the Adult Hippocampus.
%J Molecular neurobiology
%V 56
%N 7
%@ 0893-7648
%C Totowa, NJ
%I Humana Press
%M DZNE-2020-07244
%P 5188-5201
%D 2019
%X Rett syndrome is a complex neurodevelopmental disorder that is mainly caused by mutations in MECP2. However, mutations in FOXG1 cause a less frequent form of atypical Rett syndrome, called FOXG1 syndrome. FOXG1 is a key transcription factor crucial for forebrain development, where it maintains the balance between progenitor proliferation and neuronal differentiation. Using genome-wide small RNA sequencing and quantitative proteomics, we identified that FOXG1 affects the biogenesis of miR200b/a/429 and interacts with the ATP-dependent RNA helicase, DDX5/p68. Both FOXG1 and DDX5 associate with the microprocessor complex, whereby DDX5 recruits FOXG1 to DROSHA. RNA-Seq analyses of Foxg1cre/+ hippocampi and N2a cells overexpressing miR200 family members identified cAMP-dependent protein kinase type II-beta regulatory subunit (PRKAR2B) as a target of miR200 in neural cells. PRKAR2B inhibits postsynaptic functions by attenuating protein kinase A (PKA) activity; thus, increased PRKAR2B levels may contribute to neuronal dysfunctions in FOXG1 syndrome. Our data suggest that FOXG1 regulates PRKAR2B expression both on transcriptional and posttranscriptional levels.
%K Age Factors
%K Animals
%K Cyclic AMP-Dependent Protein Kinase RIIbeta Subunit: genetics
%K Cyclic AMP-Dependent Protein Kinase RIIbeta Subunit: metabolism
%K Forkhead Transcription Factors: genetics
%K Forkhead Transcription Factors: metabolism
%K Hippocampus: growth & development
%K Hippocampus: metabolism
%K Mice
%K Mice, Inbred C57BL
%K Mice, Transgenic
%K MicroRNAs: genetics
%K MicroRNAs: metabolism
%K Nerve Tissue Proteins: genetics
%K Nerve Tissue Proteins: metabolism
%K Transcription, Genetic: physiology
%K Cyclic AMP-Dependent Protein Kinase RIIbeta Subunit (NLM Chemicals)
%K Forkhead Transcription Factors (NLM Chemicals)
%K Foxg1 protein, mouse (NLM Chemicals)
%K MicroRNAs (NLM Chemicals)
%K Mirn200 microRNA, mouse (NLM Chemicals)
%K Nerve Tissue Proteins (NLM Chemicals)
%K PRKAR2B protein, human (NLM Chemicals)
%F PUB:(DE-HGF)16
%9 Journal Article
%$ pmid:30539330
%2 pmc:PMC6647430
%R 10.1007/s12035-018-1444-7
%U https://pub.dzne.de/record/140922