TY  - JOUR
AU  - Kaczmarczyk, Lech
AU  - Bansal, Vikas
AU  - Rajput, Ashish
AU  - Rahman, Raza-Ur
AU  - Krzyżak, Wiesław
AU  - Degen, Joachim
AU  - Poll, Stefanie
AU  - Fuhrmann, Martin
AU  - Bonn, Stefan
AU  - Jackson, Walker Scot
TI  - Tagger-A Swiss army knife for multiomics to dissect cell type-specific mechanisms of gene expression in mice.
JO  - PLoS biology
VL  - 17
IS  - 8
SN  - 1545-7885
CY  - Lawrence, KS
PB  - PLoS
M1  - DZNE-2020-07247
SP  - e3000374
PY  - 2019
AB  - A deep understanding of how regulation of the multiple levels of gene expression in mammalian tissues give rise to complex phenotypes has been impeded by cellular diversity. A handful of techniques were developed to tag-select nucleic acids of interest in specific cell types, thereby enabling their capture. We expanded this strategy by developing the Tagger knock-in mouse line bearing a quad-cistronic transgene combining enrichment tools for nuclei, nascent RNA, translating mRNA, and mature microRNA (miRNA). We demonstrate that Tagger can capture the desired nucleic acids, enabling multiple omics approaches to be applied to specific cell types in vivo using a single transgenic mouse line.
KW  - Animals
KW  - Cloning, Molecular: methods
KW  - Gene Expression: genetics
KW  - Gene Expression Profiling: methods
KW  - Gene Expression Regulation: genetics
KW  - Gene Knock-In Techniques
KW  - Genomics: methods
KW  - Mice
KW  - Mice, Inbred C57BL
KW  - Mice, Transgenic: genetics
KW  - MicroRNAs: genetics
KW  - Nucleic Acids: isolation & purification
KW  - Proteomics: methods
KW  - RNA, Messenger: genetics
KW  - Transcriptome: genetics
KW  - Transgenes: genetics
KW  - Whole Genome Sequencing: methods
LB  - PUB:(DE-HGF)16
C6  - pmid:31393866
C2  - pmc:PMC6701817
DO  - DOI:10.1371/journal.pbio.3000374
UR  - https://pub.dzne.de/record/140925
ER  -