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000141160 0247_ $$2doi$$a10.1002/hipo.22399
000141160 0247_ $$2pmid$$apmid:25483308
000141160 0247_ $$2ISSN$$a1050-9631
000141160 0247_ $$2ISSN$$a1098-1063
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000141160 037__ $$aDZNE-2020-07482
000141160 041__ $$aEnglish
000141160 082__ $$a610
000141160 1001_ $$aTurimella, Sada Lakshmi$$b0
000141160 245__ $$aCharacterization of cytoplasmic polyadenylation element binding 2 protein expression and its RNA binding activity.
000141160 260__ $$aNew York, NY [u.a.]$$bWiley$$c2015
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000141160 3367_ $$2ORCID$$aJOURNAL_ARTICLE
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000141160 520__ $$aCytoplasmic polyadenylation element binding (CPEB) proteins are translational regulators that are involved in the control of cellular senescence, synaptic plasticity, learning, and memory. We have previously found all four known CPEB family members to be transcribed in the mouse hippocampus. Aside from a brief description of CPEB2 in mouse brain, not much is known about its biological role. Hence, this study aims to investigate CPEB2 expression in mouse brain. With reverse transcription polymerase chain reaction (RT-PCR) of total mouse brain cDNA, we identified four distinct CPEB2 splice variants. Single-cell RT-PCR showed that CPEB2 is predominantly expressed in neurons of the juvenile and adult brain and that individual cells express different sets of splice variants. Staining of brain slices with a custom-made CPEB2 antibody revealed ubiquitous expression of the protein in many brain regions, including hippocampus, striatum, thalamus, cortex, and cerebellum. We also found differential expression of CPEB2 protein in excitatory, inhibitory, and dopaminergic neurons. In primary hippocampal cultures, the subcellular localization of CPEB2 in neurons and astrocytes resembled that of CPEB1. Electrophoretic mobility shift assay and RNA coimmunoprecipitation revealed CPEB2 interaction with β-catenin and Ca(2+) /calmodulin-dependent protein kinase II (both established CPEB1 targets), indicating an overlap in RNA binding specificity between CPEB1 and CPEB2. Furthermore, we identified ephrin receptor A4 as a putative novel target of CPEB2. In conclusion, our study identifies CPEB2 splice variants to be differentially expressed among individual cells and across cell types of the mouse hippocampus, and reveals overlapping binding specificity between CPEB2 and CPEB1.
000141160 536__ $$0G:(DE-HGF)POF3-342$$a342 - Disease Mechanisms and Model Systems (POF3-342)$$cPOF3-342$$fPOF III$$x0
000141160 542__ $$2Crossref$$i2015-09-01$$uhttp://doi.wiley.com/10.1002/tdm_license_1.1
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000141160 650_7 $$2NLM Chemicals$$aCPEB2 protein, mouse
000141160 650_7 $$2NLM Chemicals$$aCpeb1 protein, mouse
000141160 650_7 $$2NLM Chemicals$$aProtein Isoforms
000141160 650_7 $$2NLM Chemicals$$aRNA, Messenger
000141160 650_7 $$2NLM Chemicals$$aRNA-Binding Proteins
000141160 650_7 $$2NLM Chemicals$$aTranscription Factors
000141160 650_7 $$2NLM Chemicals$$abeta Catenin
000141160 650_7 $$2NLM Chemicals$$amRNA Cleavage and Polyadenylation Factors
000141160 650_7 $$063231-63-0$$2NLM Chemicals$$aRNA
000141160 650_7 $$0EC 2.7.10.1$$2NLM Chemicals$$aReceptor, EphA4
000141160 650_7 $$0EC 2.7.11.17$$2NLM Chemicals$$aCalcium-Calmodulin-Dependent Protein Kinase Type 2
000141160 650_2 $$2MeSH$$aAnimals
000141160 650_2 $$2MeSH$$aAstrocytes: metabolism
000141160 650_2 $$2MeSH$$aBrain: growth & development
000141160 650_2 $$2MeSH$$aBrain: metabolism
000141160 650_2 $$2MeSH$$aCalcium-Calmodulin-Dependent Protein Kinase Type 2: metabolism
000141160 650_2 $$2MeSH$$aHeLa Cells
000141160 650_2 $$2MeSH$$aHumans
000141160 650_2 $$2MeSH$$aMice
000141160 650_2 $$2MeSH$$aNeurons: metabolism
000141160 650_2 $$2MeSH$$aProtein Isoforms
000141160 650_2 $$2MeSH$$aRNA: metabolism
000141160 650_2 $$2MeSH$$aRNA, Messenger: metabolism
000141160 650_2 $$2MeSH$$aRNA-Binding Proteins: genetics
000141160 650_2 $$2MeSH$$aRNA-Binding Proteins: metabolism
000141160 650_2 $$2MeSH$$aReceptor, EphA4: metabolism
000141160 650_2 $$2MeSH$$aTranscription Factors: genetics
000141160 650_2 $$2MeSH$$aTranscription Factors: metabolism
000141160 650_2 $$2MeSH$$aTransfection
000141160 650_2 $$2MeSH$$abeta Catenin: metabolism
000141160 650_2 $$2MeSH$$amRNA Cleavage and Polyadenylation Factors: genetics
000141160 650_2 $$2MeSH$$amRNA Cleavage and Polyadenylation Factors: metabolism
000141160 7001_ $$aBedner, Peter$$b1
000141160 7001_ $$aSkubal, Magdalena$$b2
000141160 7001_ $$aVangoor, Vamshidhar Reddy$$b3
000141160 7001_ $$0P:(DE-2719)2810486$$aKaczmarczyk, Lech$$b4$$udzne
000141160 7001_ $$aKarl, Kevin$$b5
000141160 7001_ $$aZoidl, Georg$$b6
000141160 7001_ $$aGieselmann, Volkmar$$b7
000141160 7001_ $$aSeifert, Gerald$$b8
000141160 7001_ $$aSteinhäuser, Christian$$b9
000141160 7001_ $$aKandel, Eric$$b10
000141160 7001_ $$aTheis, Martin$$b11
000141160 77318 $$2Crossref$$3journal-article$$a10.1002/hipo.22399$$b : Wiley, 2014-12-23$$n5$$p630-642$$tHippocampus$$v25$$x1050-9631$$y2014
000141160 773__ $$0PERI:(DE-600)1498049-6$$a10.1002/hipo.22399$$gVol. 25, no. 5, p. 630 - 642$$n5$$p630-642$$q25:5<630 - 642$$tHippocampus$$v25$$x1050-9631$$y2015
000141160 8564_ $$uhttps://pub.dzne.de/record/141160/files/DZNE-2020-07482_Restricted.pdf
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000141160 9101_ $$0I:(DE-588)1065079516$$6P:(DE-2719)2810486$$aDeutsches Zentrum für Neurodegenerative Erkrankungen$$b4$$kDZNE
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000141160 9141_ $$y2015
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