%0 Journal Article
%A Badenes, Sara M
%A Fernandes, Tiago G
%A Miranda, Cláudia C
%A Pusch-Klein, Annette
%A Haupt, Simone
%A Rodrigues, Carlos AV
%A Diogo, Maria Margarida
%A Brüstle, Oliver
%A Cabral, Joaquim MS
%T Long-term expansion of human induced pluripotent stem cells in a microcarrier-based dynamic system
%J Journal of chemical technology & biotechnology
%V 92
%N 3
%@ 0268-2575
%C Chichester
%I Wiley
%M DZNE-2020-00068
%P 492-503
%D 2017
%X BACKGROUND: Human induced pluripotent stem (hiPS) cells provide a fascinating tool for exploring disease mechanisms, compound screening in pharmaceutical drug development, and might also represent a renewable source of cells for regenerative medicine applications. This requires increased cell quantities, generated under Good Manufacturing Practice-compatible conditions in a scalable system.RESULTS: A microcarrier-based suspension culture was explored for scaling-up hiPS cell expansion in serum-free medium using synthetic peptide-acrylate surface microcarriers, developed for long-term support of hiPS cell self-renewal. After a 7 day-culture in a spinner flask, cells maintained their typical morphology, pluripotency-associated marker expression and their differentiation capability. Envisaging improvement of the scalability of the culture, long-term expansion on the microcarriers was attained using confluent microcarriers as the inoculum of successive spinner flask cultures. Importantly, bead-to-bead cell transfer allowed four consecutive sub-culture procedures and a cumulative 241-fold expansion was achieved within 15 days, leading to a total viable cell number of 3.3 × 108 cells.CONCLUSION: This work is expected to enable the scale-up of hiPS cell culture under defined conditions and potentially leading to the use of pluripotent stem cell derivatives in cell replacement therapies. 
%F PUB:(DE-HGF)16
%9 Journal Article
%R 10.1002/jctb.5074
%U https://pub.dzne.de/record/144531