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000144531 1001_ $$aBadenes, Sara M$$b0
000144531 245__ $$aLong-term expansion of human induced pluripotent stem cells in a microcarrier-based dynamic system
000144531 260__ $$aChichester$$bWiley$$c2017
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000144531 520__ $$aBACKGROUND: Human induced pluripotent stem (hiPS) cells provide a fascinating tool for exploring disease mechanisms, compound screening in pharmaceutical drug development, and might also represent a renewable source of cells for regenerative medicine applications. This requires increased cell quantities, generated under Good Manufacturing Practice-compatible conditions in a scalable system.RESULTS: A microcarrier-based suspension culture was explored for scaling-up hiPS cell expansion in serum-free medium using synthetic peptide-acrylate surface microcarriers, developed for long-term support of hiPS cell self-renewal. After a 7 day-culture in a spinner flask, cells maintained their typical morphology, pluripotency-associated marker expression and their differentiation capability. Envisaging improvement of the scalability of the culture, long-term expansion on the microcarriers was attained using confluent microcarriers as the inoculum of successive spinner flask cultures. Importantly, bead-to-bead cell transfer allowed four consecutive sub-culture procedures and a cumulative 241-fold expansion was achieved within 15 days, leading to a total viable cell number of 3.3 × 108 cells.CONCLUSION: This work is expected to enable the scale-up of hiPS cell culture under defined conditions and potentially leading to the use of pluripotent stem cell derivatives in cell replacement therapies. 
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000144531 7001_ $$aFernandes, Tiago G$$b1
000144531 7001_ $$aMiranda, Cláudia C$$b2
000144531 7001_ $$aPusch-Klein, Annette$$b3
000144531 7001_ $$aHaupt, Simone$$b4
000144531 7001_ $$aRodrigues, Carlos AV$$b5
000144531 7001_ $$aDiogo, Maria Margarida$$b6
000144531 7001_ $$0P:(DE-2719)9000037$$aBrüstle, Oliver$$b7$$eCorresponding author$$udzne
000144531 7001_ $$aCabral, Joaquim MS$$b8
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000144531 773__ $$0PERI:(DE-600)1479465-2$$a10.1002/jctb.5074$$gVol. 92, no. 3, p. 492 - 503$$n3$$p492-503$$tJournal of chemical technology & biotechnology$$v92$$x0268-2575$$y2017
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