TY  - JOUR
AU  - Badenes, Sara M
AU  - Fernandes, Tiago G
AU  - Miranda, Cláudia C
AU  - Pusch-Klein, Annette
AU  - Haupt, Simone
AU  - Rodrigues, Carlos AV
AU  - Diogo, Maria Margarida
AU  - Brüstle, Oliver
AU  - Cabral, Joaquim MS
TI  - Long-term expansion of human induced pluripotent stem cells in a microcarrier-based dynamic system
JO  - Journal of chemical technology & biotechnology
VL  - 92
IS  - 3
SN  - 0268-2575
CY  - Chichester
PB  - Wiley
M1  - DZNE-2020-00068
SP  - 492-503
PY  - 2017
AB  - BACKGROUND: Human induced pluripotent stem (hiPS) cells provide a fascinating tool for exploring disease mechanisms, compound screening in pharmaceutical drug development, and might also represent a renewable source of cells for regenerative medicine applications. This requires increased cell quantities, generated under Good Manufacturing Practice-compatible conditions in a scalable system.RESULTS: A microcarrier-based suspension culture was explored for scaling-up hiPS cell expansion in serum-free medium using synthetic peptide-acrylate surface microcarriers, developed for long-term support of hiPS cell self-renewal. After a 7 day-culture in a spinner flask, cells maintained their typical morphology, pluripotency-associated marker expression and their differentiation capability. Envisaging improvement of the scalability of the culture, long-term expansion on the microcarriers was attained using confluent microcarriers as the inoculum of successive spinner flask cultures. Importantly, bead-to-bead cell transfer allowed four consecutive sub-culture procedures and a cumulative 241-fold expansion was achieved within 15 days, leading to a total viable cell number of 3.3 × 108 cells.CONCLUSION: This work is expected to enable the scale-up of hiPS cell culture under defined conditions and potentially leading to the use of pluripotent stem cell derivatives in cell replacement therapies. 
LB  - PUB:(DE-HGF)16
DO  - DOI:10.1002/jctb.5074
UR  - https://pub.dzne.de/record/144531
ER  -