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@ARTICLE{Badenes:144531,
      author       = {Badenes, Sara M and Fernandes, Tiago G and Miranda,
                      Cláudia C and Pusch-Klein, Annette and Haupt, Simone and
                      Rodrigues, Carlos AV and Diogo, Maria Margarida and
                      Brüstle, Oliver and Cabral, Joaquim MS},
      title        = {{L}ong-term expansion of human induced pluripotent stem
                      cells in a microcarrier-based dynamic system},
      journal      = {Journal of chemical technology $\&$ biotechnology},
      volume       = {92},
      number       = {3},
      issn         = {0268-2575},
      address      = {Chichester},
      publisher    = {Wiley},
      reportid     = {DZNE-2020-00068},
      pages        = {492-503},
      year         = {2017},
      abstract     = {BACKGROUND: Human induced pluripotent stem (hiPS) cells
                      provide a fascinating tool for exploring disease mechanisms,
                      compound screening in pharmaceutical drug development, and
                      might also represent a renewable source of cells for
                      regenerative medicine applications. This requires increased
                      cell quantities, generated under Good Manufacturing
                      Practice-compatible conditions in a scalable system.RESULTS:
                      A microcarrier-based suspension culture was explored for
                      scaling-up hiPS cell expansion in serum-free medium using
                      synthetic peptide-acrylate surface microcarriers, developed
                      for long-term support of hiPS cell self-renewal. After a 7
                      day-culture in a spinner flask, cells maintained their
                      typical morphology, pluripotency-associated marker
                      expression and their differentiation capability. Envisaging
                      improvement of the scalability of the culture, long-term
                      expansion on the microcarriers was attained using confluent
                      microcarriers as the inoculum of successive spinner flask
                      cultures. Importantly, bead-to-bead cell transfer allowed
                      four consecutive sub-culture procedures and a cumulative
                      241-fold expansion was achieved within 15 days, leading to a
                      total viable cell number of 3.3 × 108 cells.CONCLUSION:
                      This work is expected to enable the scale-up of hiPS cell
                      culture under defined conditions and potentially leading to
                      the use of pluripotent stem cell derivatives in cell
                      replacement therapies.},
      cin          = {Cell Programming Unit},
      ddc          = {620},
      cid          = {I:(DE-2719)1013013},
      pnm          = {344 - Clinical and Health Care Research (POF3-344)},
      pid          = {G:(DE-HGF)POF3-344},
      typ          = {PUB:(DE-HGF)16},
      doi          = {10.1002/jctb.5074},
      url          = {https://pub.dzne.de/record/144531},
}