Home > Publications Database > Long-term expansion of human induced pluripotent stem cells in a microcarrier-based dynamic system > print

001     144531
005     20250415093104.0
024 7 _ |a 10.1002/jctb.5074
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024 7 _ |a 0142-0356
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024 7 _ |a 0268-2575
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037 _ _ |a DZNE-2020-00068
082 _ _ |a 620
100 1 _ |a Badenes, Sara M
|b 0
245 _ _ |a Long-term expansion of human induced pluripotent stem cells in a microcarrier-based dynamic system
260 _ _ |a Chichester
|c 2017
|b Wiley
264 _ 1 |3 online
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|b Wiley
|c 2016-10-03
264 _ 1 |3 print
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|b Wiley
|c 2017-03-01
336 7 _ |a article
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336 7 _ |a ARTICLE
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520 _ _ |a BACKGROUND: Human induced pluripotent stem (hiPS) cells provide a fascinating tool for exploring disease mechanisms, compound screening in pharmaceutical drug development, and might also represent a renewable source of cells for regenerative medicine applications. This requires increased cell quantities, generated under Good Manufacturing Practice-compatible conditions in a scalable system.RESULTS: A microcarrier-based suspension culture was explored for scaling-up hiPS cell expansion in serum-free medium using synthetic peptide-acrylate surface microcarriers, developed for long-term support of hiPS cell self-renewal. After a 7 day-culture in a spinner flask, cells maintained their typical morphology, pluripotency-associated marker expression and their differentiation capability. Envisaging improvement of the scalability of the culture, long-term expansion on the microcarriers was attained using confluent microcarriers as the inoculum of successive spinner flask cultures. Importantly, bead-to-bead cell transfer allowed four consecutive sub-culture procedures and a cumulative 241-fold expansion was achieved within 15 days, leading to a total viable cell number of 3.3 × 108 cells.CONCLUSION: This work is expected to enable the scale-up of hiPS cell culture under defined conditions and potentially leading to the use of pluripotent stem cell derivatives in cell replacement therapies.
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542 _ _ |i 2016-10-03
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588 _ _ |a Dataset connected to CrossRef
700 1 _ |a Fernandes, Tiago G
|b 1
700 1 _ |a Miranda, Cláudia C
|b 2
700 1 _ |a Pusch-Klein, Annette
|b 3
700 1 _ |a Haupt, Simone
|b 4
700 1 _ |a Rodrigues, Carlos AV
|b 5
700 1 _ |a Diogo, Maria Margarida
|b 6
700 1 _ |a Brüstle, Oliver
|0 P:(DE-2719)9000037
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|e Corresponding author
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700 1 _ |a Cabral, Joaquim MS
|b 8
773 1 8 |a 10.1002/jctb.5074
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|n 3
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|t Journal of Chemical Technology & Biotechnology
|v 92
|y 2016
|x 0268-2575
773 _ _ |a 10.1002/jctb.5074
|g Vol. 92, no. 3, p. 492 - 503
|0 PERI:(DE-600)1479465-2
|n 3
|p 492-503
|t Journal of chemical technology & biotechnology
|v 92
|y 2017
|x 0268-2575
856 4 _ |u https://pub.dzne.de/record/144531/files/DZNE-2020-00068_Restricted.pdf
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910 1 _ |a Deutsches Zentrum für Neurodegenerative Erkrankungen
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914 1 _ |y 2017
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