000144833 001__ 144833
000144833 005__ 20250417110120.0
000144833 037__ $$aDZNE-2020-00275
000144833 041__ $$aEnglish
000144833 1001_ $$0P:(DE-2719)2810952$$aDa Silva Santos, Telma$$b0
000144833 245__ $$aAxon growth of CNS neurons in three dimensions is amoeboid-like and independent of adhesions$$f - 1900-01-01
000144833 260__ $$aBonn$$c2019
000144833 300__ $$a137 pages : 39 figures, 12 movies, CD
000144833 3367_ $$2DataCite$$aOutput Types/Dissertation
000144833 3367_ $$2ORCID$$aDISSERTATION
000144833 3367_ $$2BibTeX$$aPHDTHESIS
000144833 3367_ $$02$$2EndNote$$aThesis
000144833 3367_ $$0PUB:(DE-HGF)11$$2PUB:(DE-HGF)$$aDissertation / PhD Thesis$$bphd$$mphd$$s1744880451_17132
000144833 3367_ $$2DRIVER$$adoctoralThesis
000144833 502__ $$aDissertation, Rheinische Friedrich-Wilhelms-Universität Bonn, 2019$$bDissertation$$cRheinische Friedrich-Wilhelms-Universität Bonn$$d2019
000144833 520__ $$aNeurons physiologically grow in a three-dimensional (3D) environment. However, conventional cell culture systems for neuronal studies have been made on a hard and flat surface. Dimensionality is known to affect cytoskeletal organization, molecular pathways and cell dynamics in fibroblasts, dendritic cells and cancer cell lines. Here, I investigated axonal growth in vitro through a reproducible method representing a 3D and more physiological system. Overall neuronal growth is higher in 3D compared to the conventional two dimensions (2D) cultures, and the growth pattern matches what I observed in a more physiological set-up, ex vivo. The axonal growth cone morphology differs extensively, suggesting that artifacts arise from the mechanical properties of the 2D in vitro systems. Thus, the alterations on the actin cytoskeleton that I measured contradict the clutch model hypothesis to explain growth cone advancement. Furthermore, I provide pieces of evidence of the forces involved in physiological axonal elongation. I demonstrate that growth cone advancement is independent of adhesions, in accordance with the amoeboid movement, which is intimately linked with actin dynamics and myosin II activity. ln this work, I demonstrate how a 3D cell culture system offers insights on neuronal physiological growth and the artifacts associated with the conventional culture methods in 2D. Thereby, this work tightens the gap between in vitro andin vivo research.One Sentence Summary:The physiological motility of the axonal growth cone is amoeboid and independent of adhesions.
000144833 536__ $$0G:(DE-HGF)POF3-341$$a341 - Molecular Signaling (POF3-341)$$cPOF3-341$$fPOF III$$x0
000144833 8564_ $$uhttps://d-nb.info/1188493396
000144833 909CO $$ooai:pub.dzne.de:144833$$pVDB
000144833 9101_ $$0I:(DE-588)1065079516$$6P:(DE-2719)2810952$$aDeutsches Zentrum für Neurodegenerative Erkrankungen$$b0$$kDZNE
000144833 9131_ $$0G:(DE-HGF)POF3-341$$1G:(DE-HGF)POF3-340$$2G:(DE-HGF)POF3-300$$3G:(DE-HGF)POF3$$4G:(DE-HGF)POF$$aDE-HGF$$bGesundheit$$lErkrankungen des Nervensystems$$vMolecular Signaling$$x0
000144833 9132_ $$0G:(DE-HGF)POF4-899$$1G:(DE-HGF)POF4-890$$2G:(DE-HGF)POF4-800$$3G:(DE-HGF)POF4$$4G:(DE-HGF)POF$$aDE-HGF$$bProgrammungebundene Forschung$$lohne Programm$$vohne Topic$$x0
000144833 9141_ $$y2019
000144833 920__ $$lyes
000144833 9201_ $$0I:(DE-2719)1013002$$kAG Bradke$$lAxon Growth and Regeneration$$x0
000144833 980__ $$aphd
000144833 980__ $$aVDB
000144833 980__ $$aI:(DE-2719)1013002
000144833 980__ $$aUNRESTRICTED