000151529 001__ 151529 000151529 005__ 20230915093956.0 000151529 0247_ $$2pmc$$apmc:PMC7687380 000151529 0247_ $$2doi$$a10.1038/s41586-020-2156-5 000151529 0247_ $$2ISSN$$a0028-0836 000151529 0247_ $$2ISSN$$a0300-8746 000151529 0247_ $$2ISSN$$a1476-4687 000151529 0247_ $$2ISSN$$a2058-1106 000151529 0247_ $$2altmetric$$aaltmetric:78679731 000151529 0247_ $$2pmid$$apmid:32296178 000151529 037__ $$aDZNE-2020-01113 000151529 041__ $$aEnglish 000151529 082__ $$a530 000151529 1001_ $$aRauch, Jennifer N.$$b0 000151529 245__ $$aLRP1 is a master regulator of tau uptake and spread 000151529 260__ $$aLondon$$bMacmillan28177$$c2020 000151529 264_1 $$2Crossref$$3online$$bSpringer Science and Business Media LLC$$c2020-04-01 000151529 264_1 $$2Crossref$$3print$$bSpringer Science and Business Media LLC$$c2020-04-01 000151529 3367_ $$2DRIVER$$aarticle 000151529 3367_ $$2DataCite$$aOutput Types/Journal article 000151529 3367_ $$0PUB:(DE-HGF)16$$2PUB:(DE-HGF)$$aJournal Article$$bjournal$$mjournal$$s1600944130_14349 000151529 3367_ $$2BibTeX$$aARTICLE 000151529 3367_ $$2ORCID$$aJOURNAL_ARTICLE 000151529 3367_ $$00$$2EndNote$$aJournal Article 000151529 520__ $$aThe spread of protein aggregates during disease progression is a common theme underlying many neurodegenerative diseases. The microtubule-associated protein tau has a central role in the pathogenesis of several forms of dementia known as tauopathies—including Alzheimer’s disease, frontotemporal dementia and chronic traumatic encephalopathy1. Progression of these diseases is characterized by the sequential spread and deposition of protein aggregates in a predictable pattern that correlates with clinical severity2. This observation and complementary experimental studies3,4 have suggested that tau can spread in a prion-like manner, by passing to naive cells in which it templates misfolding and aggregation. However, although the propagation of tau has been extensively studied, the underlying cellular mechanisms remain poorly understood. Here we show that the low-density lipoprotein receptor-related protein 1 (LRP1) controls the endocytosis of tau and its subsequent spread. Knockdown of LRP1 significantly reduced tau uptake in H4 neuroglioma cells and in induced pluripotent stem cell-derived neurons. The interaction between tau and LRP1 is mediated by lysine residues in the microtubule-binding repeat region of tau. Furthermore, downregulation of LRP1 in an in vivo mouse model of tau spread was found to effectively reduce the propagation of tau between neurons. Our results identify LRP1 as a key regulator of tau spread in the brain, and therefore a potential target for the treatment of diseases that involve tau spread and aggregation. 000151529 536__ $$0G:(DE-HGF)POF3-342$$a342 - Disease Mechanisms and Model Systems (POF3-342)$$cPOF3-342$$fPOF III$$x0 000151529 542__ $$2Crossref$$i2020-04-01$$uhttp://www.springer.com/tdm 000151529 588__ $$aDataset connected to CrossRef 000151529 650_2 $$2MeSH$$aAnimals 000151529 650_2 $$2MeSH$$aCell Line 000151529 650_2 $$2MeSH$$aEndocytosis 000151529 650_2 $$2MeSH$$aFemale 000151529 650_2 $$2MeSH$$aHumans 000151529 650_2 $$2MeSH$$aLigands 000151529 650_2 $$2MeSH$$aLow Density Lipoprotein Receptor-Related Protein-1: genetics 000151529 650_2 $$2MeSH$$aLow Density Lipoprotein Receptor-Related Protein-1: metabolism 000151529 650_2 $$2MeSH$$aMale 000151529 650_2 $$2MeSH$$aMice 000151529 650_2 $$2MeSH$$aNeurons: metabolism 000151529 650_2 $$2MeSH$$atau Proteins: metabolism 000151529 7001_ $$aLuna, Gabriel$$b1 000151529 7001_ $$aGuzman, Elmer$$b2 000151529 7001_ $$aAudouard, Morgane$$b3 000151529 7001_ $$aChallis, Collin$$b4 000151529 7001_ $$00000-0002-0839-5428$$aSibih, Youssef E.$$b5 000151529 7001_ $$aLeshuk, Carolina$$b6 000151529 7001_ $$aHernandez, Israel$$b7 000151529 7001_ $$0P:(DE-2719)2812695$$aWegmann, Susanne$$b8$$udzne 000151529 7001_ $$00000-0002-7959-9401$$aHyman, Bradley T.$$b9 000151529 7001_ $$00000-0001-5868-348X$$aGradinaru, Viviana$$b10 000151529 7001_ $$00000-0002-3819-7019$$aKampmann, Martin$$b11 000151529 7001_ $$0P:(DE-HGF)0$$aKosik, Kenneth S.$$b12$$eCorresponding author 000151529 77318 $$2Crossref$$3journal-article$$a10.1038/s41586-020-2156-5$$b : Springer Science and Business Media LLC, 2020-04-01$$n7803$$p381-385$$tNature$$v580$$x0028-0836$$y2020 000151529 773__ $$0PERI:(DE-600)2590711-6$$a10.1038/s41586-020-2156-5$$gVol. 580, no. 7803, p. 381 - 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