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000151549 037__ $$aDZNE-2020-01133
000151549 041__ $$aEnglish
000151549 082__ $$a610
000151549 1001_ $$aMüller-Komorowska, Daniel$$b0
000151549 245__ $$aNonspecific Expression in Limited Excitatory Cell Populations in Interneuron-Targeting Cre-driver Lines Can Have Large Functional Effects
000151549 260__ $$aLausanne$$bFrontiers Research Foundation$$c2020
000151549 264_1 $$2Crossref$$3online$$bFrontiers Media SA$$c2020-04-27
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000151549 520__ $$aTransgenic Cre-recombinase expressing mouse lines are widely used to express fluorescent proteins and opto-/chemogenetic actuators, making them a cornerstone of modern neuroscience. The investigation of interneurons in particular has benefitted from the ability to genetically target specific cell types. However, the specificity of some Cre driver lines has been called into question. Here, we show that nonspecific expression in a subset of hippocampal neurons can have substantial nonspecific functional effects in a somatostatin-Cre (SST-Cre) mouse line. Nonspecific targeting of CA3 pyramidal cells caused large optogenetically evoked excitatory currents in remote brain regions. Similar, but less severe patterns of nonspecific expression were observed in a widely used SST-IRES-Cre line, when crossed with a reporter mouse line. Viral transduction on the other hand yielded more specific expression but still resulted in nonspecific expression in a minority of pyramidal layer cells. These results suggest that a careful analysis of specificity is mandatory before the use of Cre driver lines for opto- or chemogenetic manipulation approaches.
000151549 536__ $$0G:(DE-HGF)POF3-342$$a342 - Disease Mechanisms and Model Systems (POF3-342)$$cPOF3-342$$fPOF III$$x0
000151549 542__ $$2Crossref$$i2020-04-27$$uhttps://creativecommons.org/licenses/by/4.0/
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000151549 650_2 $$2MeSH$$aAnimals
000151549 650_2 $$2MeSH$$aCA3 Region, Hippocampal: chemistry
000151549 650_2 $$2MeSH$$aCA3 Region, Hippocampal: cytology
000151549 650_2 $$2MeSH$$aCA3 Region, Hippocampal: metabolism
000151549 650_2 $$2MeSH$$aGene Expression
000151549 650_2 $$2MeSH$$aIntegrases: analysis
000151549 650_2 $$2MeSH$$aIntegrases: biosynthesis
000151549 650_2 $$2MeSH$$aIntegrases: genetics
000151549 650_2 $$2MeSH$$aInterneurons: chemistry
000151549 650_2 $$2MeSH$$aInterneurons: metabolism
000151549 650_2 $$2MeSH$$aMice
000151549 650_2 $$2MeSH$$aMice, Inbred C57BL
000151549 650_2 $$2MeSH$$aMice, Transgenic
000151549 650_2 $$2MeSH$$aOptogenetics: methods
000151549 650_2 $$2MeSH$$aSomatostatin: analysis
000151549 650_2 $$2MeSH$$aSomatostatin: biosynthesis
000151549 650_2 $$2MeSH$$aSomatostatin: genetics
000151549 7001_ $$0P:(DE-HGF)0$$aOpitz, Thoralf$$b1
000151549 7001_ $$aElzoheiry, Shehabeldin$$b2
000151549 7001_ $$aSchweizer, Michaela$$b3
000151549 7001_ $$0P:(DE-2719)2811489$$aAmbrad Giovannetti, Eleonora$$b4$$udzne
000151549 7001_ $$0P:(DE-HGF)0$$aBeck, Heinz$$b5$$eCorresponding author
000151549 77318 $$2Crossref$$3journal-article$$a10.3389/fncir.2020.00016$$b : Frontiers Media SA, 2020-04-27$$tFrontiers in Neural Circuits$$v14$$x1662-5110$$y2020
000151549 773__ $$0PERI:(DE-600)2452968-0$$a10.3389/fncir.2020.00016$$gVol. 14, p. 16$$p16$$tFrontiers in neural circuits$$v14$$x1662-5110$$y2020
000151549 8564_ $$uhttps://www.frontiersin.org/articles/10.3389/fncir.2020.00016/full
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000151549 9141_ $$y2020
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