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@ARTICLE{MllerKomorowska:151549,
      author       = {Müller-Komorowska, Daniel and Opitz, Thoralf and
                      Elzoheiry, Shehabeldin and Schweizer, Michaela and Ambrad
                      Giovannetti, Eleonora and Beck, Heinz},
      title        = {{N}onspecific {E}xpression in {L}imited {E}xcitatory {C}ell
                      {P}opulations in {I}nterneuron-{T}argeting {C}re-driver
                      {L}ines {C}an {H}ave {L}arge {F}unctional {E}ffects},
      journal      = {Frontiers in neural circuits},
      volume       = {14},
      issn         = {1662-5110},
      address      = {Lausanne},
      publisher    = {Frontiers Research Foundation},
      reportid     = {DZNE-2020-01133},
      pages        = {16},
      year         = {2020},
      abstract     = {Transgenic Cre-recombinase expressing mouse lines are
                      widely used to express fluorescent proteins and
                      opto-/chemogenetic actuators, making them a cornerstone of
                      modern neuroscience. The investigation of interneurons in
                      particular has benefitted from the ability to genetically
                      target specific cell types. However, the specificity of some
                      Cre driver lines has been called into question. Here, we
                      show that nonspecific expression in a subset of hippocampal
                      neurons can have substantial nonspecific functional effects
                      in a somatostatin-Cre (SST-Cre) mouse line. Nonspecific
                      targeting of CA3 pyramidal cells caused large
                      optogenetically evoked excitatory currents in remote brain
                      regions. Similar, but less severe patterns of nonspecific
                      expression were observed in a widely used SST-IRES-Cre line,
                      when crossed with a reporter mouse line. Viral transduction
                      on the other hand yielded more specific expression but still
                      resulted in nonspecific expression in a minority of
                      pyramidal layer cells. These results suggest that a careful
                      analysis of specificity is mandatory before the use of Cre
                      driver lines for opto- or chemogenetic manipulation
                      approaches.},
      keywords     = {Animals / CA3 Region, Hippocampal: chemistry / CA3 Region,
                      Hippocampal: cytology / CA3 Region, Hippocampal: metabolism
                      / Gene Expression / Integrases: analysis / Integrases:
                      biosynthesis / Integrases: genetics / Interneurons:
                      chemistry / Interneurons: metabolism / Mice / Mice, Inbred
                      C57BL / Mice, Transgenic / Optogenetics: methods /
                      Somatostatin: analysis / Somatostatin: biosynthesis /
                      Somatostatin: genetics},
      cin          = {U Preclinical Researchers - Bonn},
      ddc          = {610},
      cid          = {I:(DE-2719)7000005},
      pnm          = {342 - Disease Mechanisms and Model Systems (POF3-342)},
      pid          = {G:(DE-HGF)POF3-342},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:32395103},
      pmc          = {pmc:PMC7197702},
      doi          = {10.3389/fncir.2020.00016},
      url          = {https://pub.dzne.de/record/151549},
}