Home > Publications Database > Nonspecific Expression in Limited Excitatory Cell Populations in Interneuron-Targeting Cre-driver Lines Can Have Large Functional Effects > print

001     151549
005     20230915093958.0
024 7 _ |a pmid:32395103
|2 pmid
024 7 _ |a 10.3389/fncir.2020.00016
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037 _ _ |a DZNE-2020-01133
041 _ _ |a English
082 _ _ |a 610
100 1 _ |a Müller-Komorowska, Daniel
|b 0
245 _ _ |a Nonspecific Expression in Limited Excitatory Cell Populations in Interneuron-Targeting Cre-driver Lines Can Have Large Functional Effects
260 _ _ |a Lausanne
|c 2020
|b Frontiers Research Foundation
264 _ 1 |3 online
|2 Crossref
|b Frontiers Media SA
|c 2020-04-27
336 7 _ |a article
|2 DRIVER
336 7 _ |a Output Types/Journal article
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336 7 _ |a Journal Article
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|m journal
|0 PUB:(DE-HGF)16
|s 1601988930_12125
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336 7 _ |a ARTICLE
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336 7 _ |a Journal Article
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520 _ _ |a Transgenic Cre-recombinase expressing mouse lines are widely used to express fluorescent proteins and opto-/chemogenetic actuators, making them a cornerstone of modern neuroscience. The investigation of interneurons in particular has benefitted from the ability to genetically target specific cell types. However, the specificity of some Cre driver lines has been called into question. Here, we show that nonspecific expression in a subset of hippocampal neurons can have substantial nonspecific functional effects in a somatostatin-Cre (SST-Cre) mouse line. Nonspecific targeting of CA3 pyramidal cells caused large optogenetically evoked excitatory currents in remote brain regions. Similar, but less severe patterns of nonspecific expression were observed in a widely used SST-IRES-Cre line, when crossed with a reporter mouse line. Viral transduction on the other hand yielded more specific expression but still resulted in nonspecific expression in a minority of pyramidal layer cells. These results suggest that a careful analysis of specificity is mandatory before the use of Cre driver lines for opto- or chemogenetic manipulation approaches.
536 _ _ |a 342 - Disease Mechanisms and Model Systems (POF3-342)
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542 _ _ |i 2020-04-27
|2 Crossref
|u https://creativecommons.org/licenses/by/4.0/
588 _ _ |a Dataset connected to CrossRef
650 _ 2 |a Animals
|2 MeSH
650 _ 2 |a CA3 Region, Hippocampal: chemistry
|2 MeSH
650 _ 2 |a CA3 Region, Hippocampal: cytology
|2 MeSH
650 _ 2 |a CA3 Region, Hippocampal: metabolism
|2 MeSH
650 _ 2 |a Gene Expression
|2 MeSH
650 _ 2 |a Integrases: analysis
|2 MeSH
650 _ 2 |a Integrases: biosynthesis
|2 MeSH
650 _ 2 |a Integrases: genetics
|2 MeSH
650 _ 2 |a Interneurons: chemistry
|2 MeSH
650 _ 2 |a Interneurons: metabolism
|2 MeSH
650 _ 2 |a Mice
|2 MeSH
650 _ 2 |a Mice, Inbred C57BL
|2 MeSH
650 _ 2 |a Mice, Transgenic
|2 MeSH
650 _ 2 |a Optogenetics: methods
|2 MeSH
650 _ 2 |a Somatostatin: analysis
|2 MeSH
650 _ 2 |a Somatostatin: biosynthesis
|2 MeSH
650 _ 2 |a Somatostatin: genetics
|2 MeSH
700 1 _ |a Opitz, Thoralf
|0 P:(DE-HGF)0
|b 1
700 1 _ |a Elzoheiry, Shehabeldin
|b 2
700 1 _ |a Schweizer, Michaela
|b 3
700 1 _ |a Ambrad Giovannetti, Eleonora
|0 P:(DE-2719)2811489
|b 4
|u dzne
700 1 _ |a Beck, Heinz
|0 P:(DE-HGF)0
|b 5
|e Corresponding author
773 1 8 |a 10.3389/fncir.2020.00016
|b : Frontiers Media SA, 2020-04-27
|3 journal-article
|2 Crossref
|t Frontiers in Neural Circuits
|v 14
|y 2020
|x 1662-5110
773 _ _ |a 10.3389/fncir.2020.00016
|g Vol. 14, p. 16
|0 PERI:(DE-600)2452968-0
|p 16
|t Frontiers in neural circuits
|v 14
|y 2020
|x 1662-5110
856 4 _ |u https://www.frontiersin.org/articles/10.3389/fncir.2020.00016/full
856 4 _ |u https://pub.dzne.de/record/151549/files/6915.pdf
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910 1 _ |a Deutsches Zentrum für Neurodegenerative Erkrankungen
|0 I:(DE-588)1065079516
|k DZNE
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913 1 _ |a DE-HGF
|b Forschungsbereich Gesundheit
|l Erkrankungen des Nervensystems
|1 G:(DE-HGF)POF3-340
|0 G:(DE-HGF)POF3-342
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|v Disease Mechanisms and Model Systems
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914 1 _ |y 2020
915 _ _ |a Creative Commons Attribution CC BY (No Version)
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