TY  - JOUR
AU  - Arora, Amandeep Singh
AU  - Zafar, Saima
AU  - Latif, Umair
AU  - Llorens, Franc
AU  - Sabine, Mihm
AU  - Kumar, Prateek
AU  - Tahir, Waqas
AU  - Thüne, Katrin
AU  - Shafiq, Mohsin
AU  - Schmitz, Matthias
AU  - Zerr, Inga
TI  - The role of cellular prion protein in lipid metabolism in the liver.
JO  - Prion
VL  - 14
IS  - 1
SN  - 1933-690X
CY  - London [u.a.]
PB  - Taylor & Francis
M1  - DZNE-2020-01224
SP  - 95 - 108
PY  - 2020
AB  - Cellular prion protein (PrPC) is a plasma membrane glycophosphatidylinositol-anchored protein and it is involved in multiple functions, including neuroprotection and oxidative stress. So far, most of the PrPC functional research is done in neuronal tissue or cell lines; the role of PrPC in non-neuronal tissues such as liver is only poorly understood. To characterize the role of PrPC in the liver, a proteomics approach was applied in the liver tissue of PrPC knockout mice. The proteome analysis and biochemical validations showed an excessive fat accumulation in the liver of PrPC knockout mice with a change in mRNA expression of genes linked to lipid metabolism. In addition, the higher Bax to Bcl2 ratio, up-regulation of tgfb1 mRNA expression in PrPC knockout mice liver, further showed the evidences of metabolic disease. Over-expression of PrPC in fatty acid-treated AML12 hepatic cell line caused a reduction in excessive intracellular fat accumulation; shows association of PrPC levels and lipid metabolism. Therefore, based on observation of excessive fat globules in the liver of ageing PrPC knockout mice and the reduction of fat accumulation in AML12 cell line with PrPC over-expression, the role of PrPC in lipid metabolism is described.
KW  - Acetyl-CoA Carboxylase: genetics
KW  - Acetyl-CoA Carboxylase: metabolism
KW  - Adiposity
KW  - Animals
KW  - Cell Line
KW  - Electrophoresis, Gel, Two-Dimensional
KW  - Fatty Acid Synthases: genetics
KW  - Fatty Acid Synthases: metabolism
KW  - Female
KW  - Gene Expression Regulation
KW  - Lipid Metabolism: genetics
KW  - Liver: metabolism
KW  - Male
KW  - Metabolic Diseases: metabolism
KW  - Mice, Inbred C57BL
KW  - Mice, Knockout
KW  - PPAR alpha: metabolism
KW  - Prion Proteins: metabolism
KW  - Proteome: metabolism
KW  - Proteomics
KW  - RNA, Messenger: genetics
KW  - RNA, Messenger: metabolism
KW  - Transforming Growth Factor beta1: genetics
KW  - Transforming Growth Factor beta1: metabolism
KW  - Triglycerides: metabolism
LB  - PUB:(DE-HGF)16
C6  - pmid:32138593
C2  - pmc:PMC7153832
DO  - DOI:10.1080/19336896.2020.1729074
UR  - https://pub.dzne.de/record/151645
ER  -