TY - JOUR AU - Wickel, Jonathan AU - Chung, Ha-Yeun AU - Platzer, Stephanie AU - Lehmann, Thomas AU - Prüss, Harald AU - Leypoldt, Frank AU - Günther, Albrecht AU - Scherag, André AU - Geis, Christian TI - Generate-Boost: study protocol for a prospective, multicenter, randomized controlled, double-blinded phase II trial to evaluate efficacy and safety of bortezomib in patients with severe autoimmune encephalitis JO - Trials VL - 21 IS - 1 SN - 1745-6215 CY - London PB - BioMed Central M1 - DZNE-2020-01276 SP - 625 PY - 2020 AB - BackgroundAutoimmune encephalitis is a new spectrum of autoimmune disorders of the central nervous system (CNS), which are characterized by pathogenic autoantibodies against neuronal surface antigens. Clinical presentations range from acute to subacute encephalopathy with neurological and psychiatric symptoms, and life-threatening autonomic dysfunction in severe cases. There exist no approved therapies nor is data available from controlled clinical trials. Patients are usually treated with diverse combinations of immunotherapy. However, effect of immunotherapy on antibody-producing cells and thus on levels of pathogenic autoantibodies is insufficient. Therefore, therapeutic response is sometimes prolonged with necessity of long-time intensive care treatment and also irreversible deficits occur in severe cases. This trial will investigate the efficacy and safety of bortezomib, a proteasome inhibitor known to selectively deplete plasma cells, in patients with severe autoimmune encephalitis who have been treated with rituximab with insufficient response.MethodsGenerate-Boost is an investigator-initiated, multicenter, double-blinded, randomized controlled phase II trial which will be conducted in specialized neurological hospitals within the GENERATE (GErman NEtwork for Research on AuToimmune Encephalitis) network in Germany. Adult patients with severe autoimmune encephalitis (modified Rankin scale, mRS ≥ 3), autoantibodies against neuronal surface antigens, and pretreatment with rituximab are eligible for study participation. Fifty patients will be randomized 1:1 and undergo up to 3 cycles (each 21 days with 4 s. c. applications) of bortezomib or placebo. All patients will receive concomitant medication with dexamethasone, acyclovir and co-trimoxazole. The primary efficacy endpoint is the mRS score 17 weeks after first treatment application. Secondary endpoints are neurocognitive function, antibody titers, markers of neuronal cell damage, length of ICU/hospital stay, and mRS and Glasgow coma scale scores throughout the trial up to week 17. General and bortezomib-specific adverse events are monitored continuously.DiscussionThe expected outcome of the study is to obtain first reliable data on a hypothesis-driven therapeutic option in severe and difficult-to-treat autoimmune encephalitis. If treatment with bortezomib is beneficial in these cases, this will be the basis for implementation in the current guidelines. KW - Acyclovir KW - Adult KW - Autoantibodies: blood KW - Bortezomib: adverse effects KW - Bortezomib: therapeutic use KW - Clinical Trials, Phase II as Topic KW - Dexamethasone KW - Double-Blind Method KW - Drug Therapy, Combination KW - Encephalitis: drug therapy KW - Encephalitis: immunology KW - Germany KW - Glasgow Coma Scale KW - Hashimoto Disease: drug therapy KW - Hashimoto Disease: immunology KW - Humans KW - Immunotherapy KW - Multicenter Studies as Topic KW - Prospective Studies KW - Proteasome Inhibitors: adverse effects KW - Proteasome Inhibitors: therapeutic use KW - Randomized Controlled Trials as Topic KW - Time Factors KW - Treatment Outcome KW - Trimethoprim, Sulfamethoxazole Drug Combination LB - PUB:(DE-HGF)16 C2 - pmc:PMC7346383 C6 - pmid:32641101 DO - DOI:10.1186/s13063-020-04516-7 UR - https://pub.dzne.de/record/153279 ER -