Home > Publications Database > Axon Growth of CNS Neurons in Three Dimensions Is Amoeboid and Independent of Adhesions > print

001     153281
005     20240223115101.0
024 7 _ |a 10.1016/j.celrep.2020.107907
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024 7 _ |a 2211-1247
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024 7 _ |a 2639-1856
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037 _ _ |a DZNE-2020-01278
041 _ _ |a English
082 _ _ |a 610
100 1 _ |a Da Silva Santos, Telma
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245 _ _ |a Axon Growth of CNS Neurons in Three Dimensions Is Amoeboid and Independent of Adhesions
260 _ _ |a [New York, NY]
|c 2020
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520 _ _ |a SummaryDuring development of the central nervous system (CNS), neurons polarize and rapidly extend their axons to assemble neuronal circuits. The growth cone leads the axon to its target and drives axon growth. Here, we explored the mechanisms underlying axon growth in three dimensions. Live in situ imaging and super-resolution microscopy combined with pharmacological and molecular manipulations as well as biophysical force measurements revealed that growth cones extend CNS axons independent of pulling forces on their substrates and without the need for adhesions in three-dimensional (3D) environments. In 3D, microtubules grow unrestrained from the actomyosin cytoskeleton into the growth cone leading edge to enable rapid axon extension. Axons extend and polarize even in adhesion-inert matrices. Thus, CNS neurons use amoeboid mechanisms to drive axon growth. Together with our understanding that adult CNS axons regenerate by reactivating developmental processes, our findings illuminate how cytoskeletal manipulations enable axon regeneration in the adult CNS.
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650 _ 2 |a Actins: metabolism
|2 MeSH
650 _ 2 |a Actomyosin: metabolism
|2 MeSH
650 _ 2 |a Animals
|2 MeSH
650 _ 2 |a Axons: metabolism
|2 MeSH
650 _ 2 |a Cell Adhesion
|2 MeSH
650 _ 2 |a Cell Polarity
|2 MeSH
650 _ 2 |a Central Nervous System: metabolism
|2 MeSH
650 _ 2 |a Collagen: metabolism
|2 MeSH
650 _ 2 |a Fibroblasts: metabolism
|2 MeSH
650 _ 2 |a Growth Cones: metabolism
|2 MeSH
650 _ 2 |a Hippocampus: embryology
|2 MeSH
650 _ 2 |a Mice, Inbred C57BL
|2 MeSH
650 _ 2 |a Microtubules: metabolism
|2 MeSH
650 _ 2 |a Neuronal Outgrowth
|2 MeSH
650 _ 2 |a Polymerization
|2 MeSH
700 1 _ |a Schaffran, Barbara
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700 1 _ |a Broguière, Nicolas
|b 2
700 1 _ |a Meyn, Liane
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700 1 _ |a Zenobi-Wong, Marcy
|b 4
700 1 _ |a Bradke, Frank
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|e Last author
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773 _ _ |a 10.1016/j.celrep.2020.107907
|g Vol. 32, no. 3, p. 107907 -
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|t Cell reports
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856 4 _ |u https://www.sciencedirect.com/science/article/pii/S2211124720308883
856 4 _ |u https://pub.dzne.de/record/153281/files/DZNE-2020-01278.pdf
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910 1 _ |a Deutsches Zentrum für Neurodegenerative Erkrankungen
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910 1 _ |a Deutsches Zentrum für Neurodegenerative Erkrankungen
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910 1 _ |a Deutsches Zentrum für Neurodegenerative Erkrankungen
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