000153368 001__ 153368
000153368 005__ 20240321221042.0
000153368 0247_ $$2doi$$a10.1038/s41590-020-0707-4
000153368 0247_ $$2pmid$$apmid:32541832
000153368 0247_ $$2ISSN$$a1529-2908
000153368 0247_ $$2ISSN$$a1529-2916
000153368 0247_ $$2altmetric$$aaltmetric:84050812
000153368 037__ $$aDZNE-2020-01365
000153368 041__ $$aEnglish
000153368 082__ $$a610
000153368 1001_ $$0P:(DE-HGF)0$$aMasuda, Takahiro$$b0$$eCorresponding author
000153368 245__ $$aNovel Hexb-based tools for studying microglia in the CNS.
000153368 260__ $$aLondon$$bSpringer Nature Limited$$c2020
000153368 3367_ $$2DRIVER$$aarticle
000153368 3367_ $$2DataCite$$aOutput Types/Journal article
000153368 3367_ $$0PUB:(DE-HGF)16$$2PUB:(DE-HGF)$$aJournal Article$$bjournal$$mjournal$$s1704807496_29464
000153368 3367_ $$2BibTeX$$aARTICLE
000153368 3367_ $$2ORCID$$aJOURNAL_ARTICLE
000153368 3367_ $$00$$2EndNote$$aJournal Article
000153368 520__ $$aMicroglia and central nervous system (CNS)-associated macrophages (CAMs), such as perivascular and meningeal macrophages, are implicated in virtually all diseases of the CNS. However, little is known about their cell-type-specific roles in the absence of suitable tools that would allow for functional discrimination between the ontogenetically closely related microglia and CAMs. To develop a new microglia gene targeting model, we first applied massively parallel single-cell analyses to compare microglia and CAM signatures during homeostasis and disease and identified hexosaminidase subunit beta (Hexb) as a stably expressed microglia core gene, whereas other microglia core genes were substantially downregulated during pathologies. Next, we generated HexbtdTomato mice to stably monitor microglia behavior in vivo. Finally, the Hexb locus was employed for tamoxifen-inducible Cre-mediated gene manipulation in microglia and for fate mapping of microglia but not CAMs. In sum, we provide valuable new genetic tools to specifically study microglia functions in the CNS.
000153368 536__ $$0G:(DE-HGF)POF3-344$$a344 - Clinical and Health Care Research (POF3-344)$$cPOF3-344$$fPOF III$$x0
000153368 588__ $$aDataset connected to CrossRef, PubMed,
000153368 650_7 $$2NLM Chemicals$$aLuminescent Agents
000153368 650_7 $$2NLM Chemicals$$aLuminescent Proteins
000153368 650_7 $$2NLM Chemicals$$ared fluorescent protein
000153368 650_7 $$0EC 3.2.1.52$$2NLM Chemicals$$abeta-Hexosaminidase beta Chain
000153368 650_2 $$2MeSH$$aAnimals
000153368 650_2 $$2MeSH$$aBrain: cytology
000153368 650_2 $$2MeSH$$aBrain: immunology
000153368 650_2 $$2MeSH$$aBrain: pathology
000153368 650_2 $$2MeSH$$aCRISPR-Cas Systems: genetics
000153368 650_2 $$2MeSH$$aEncephalomyelitis, Autoimmune, Experimental: immunology
000153368 650_2 $$2MeSH$$aEncephalomyelitis, Autoimmune, Experimental: pathology
000153368 650_2 $$2MeSH$$aFacial Nerve Injuries: immunology
000153368 650_2 $$2MeSH$$aFacial Nerve Injuries: pathology
000153368 650_2 $$2MeSH$$aGene Knock-In Techniques
000153368 650_2 $$2MeSH$$aGenes, Reporter: genetics
000153368 650_2 $$2MeSH$$aGenetic Loci: genetics
000153368 650_2 $$2MeSH$$aHumans
000153368 650_2 $$2MeSH$$aIntravital Microscopy
000153368 650_2 $$2MeSH$$aLuminescent Agents: chemistry
000153368 650_2 $$2MeSH$$aLuminescent Proteins: chemistry
000153368 650_2 $$2MeSH$$aLuminescent Proteins: genetics
000153368 650_2 $$2MeSH$$aMacrophages: immunology
000153368 650_2 $$2MeSH$$aMacrophages: metabolism
000153368 650_2 $$2MeSH$$aMice
000153368 650_2 $$2MeSH$$aMicroglia: immunology
000153368 650_2 $$2MeSH$$aMicroglia: metabolism
000153368 650_2 $$2MeSH$$aNIH 3T3 Cells
000153368 650_2 $$2MeSH$$aRNA-Seq
000153368 650_2 $$2MeSH$$aSingle-Cell Analysis
000153368 650_2 $$2MeSH$$aTransfection
000153368 650_2 $$2MeSH$$abeta-Hexosaminidase beta Chain: genetics
000153368 650_2 $$2MeSH$$abeta-Hexosaminidase beta Chain: metabolism
000153368 7001_ $$0P:(DE-HGF)0$$aAmann, Lukas$$b1
000153368 7001_ $$0P:(DE-HGF)0$$aSankowski, Roman$$b2
000153368 7001_ $$0P:(DE-HGF)0$$aStaszewski, Ori$$b3
000153368 7001_ $$0P:(DE-HGF)0$$aLenz, Maximilian$$b4
000153368 7001_ $$0P:(DE-HGF)0$$aD Errico, Paolo$$b5
000153368 7001_ $$0P:(DE-HGF)0$$aSnaidero, Nicolas$$b6
000153368 7001_ $$0P:(DE-HGF)0$$aCosta Jordão, Marta Joana$$b7
000153368 7001_ $$0P:(DE-HGF)0$$aBöttcher, Chotima$$b8
000153368 7001_ $$0P:(DE-HGF)0$$aKierdorf, Katrin$$b9
000153368 7001_ $$0P:(DE-HGF)0$$aJung, Steffen$$b10
000153368 7001_ $$0P:(DE-2719)2811122$$aPriller, Josef$$b11$$udzne
000153368 7001_ $$0P:(DE-HGF)0$$aMisgeld, Thomas$$b12
000153368 7001_ $$0P:(DE-HGF)0$$aVlachos, Andreas$$b13
000153368 7001_ $$0P:(DE-HGF)0$$aMeyer-Luehmann, Melanie$$b14
000153368 7001_ $$0P:(DE-HGF)0$$aKnobeloch, Klaus-Peter$$b15
000153368 7001_ $$0P:(DE-HGF)0$$aPrinz, Marco$$b16
000153368 773__ $$0PERI:(DE-600)2026412-4$$a10.1038/s41590-020-0707-4$$gVol. 21, no. 7, p. 802 - 815$$n7$$p802 - 815$$tNature immunology$$v21$$x1529-2916$$y2020
000153368 8564_ $$uhttps://pub.dzne.de/record/153368/files/DZNE-2020-01365.pdf
000153368 8564_ $$uhttps://pub.dzne.de/record/153368/files/DZNE-2020-01365.pdf?subformat=pdfa$$xpdfa
000153368 909CO $$ooai:pub.dzne.de:153368$$pVDB
000153368 9101_ $$0I:(DE-588)1065079516$$6P:(DE-2719)2811122$$aDeutsches Zentrum für Neurodegenerative Erkrankungen$$b11$$kDZNE
000153368 9131_ $$0G:(DE-HGF)POF3-344$$1G:(DE-HGF)POF3-340$$2G:(DE-HGF)POF3-300$$3G:(DE-HGF)POF3$$4G:(DE-HGF)POF$$aDE-HGF$$bGesundheit$$lErkrankungen des Nervensystems$$vClinical and Health Care Research$$x0
000153368 9141_ $$y2020
000153368 915__ $$0StatID:(DE-HGF)0160$$2StatID$$aDBCoverage$$bEssential Science Indicators$$d2020-08-29
000153368 915__ $$0StatID:(DE-HGF)1190$$2StatID$$aDBCoverage$$bBiological Abstracts$$d2020-08-29
000153368 915__ $$0StatID:(DE-HGF)0113$$2StatID$$aWoS$$bScience Citation Index Expanded$$d2020-08-29
000153368 915__ $$0StatID:(DE-HGF)0420$$2StatID$$aNationallizenz$$d2023-03-30$$wger
000153368 915__ $$0StatID:(DE-HGF)0100$$2StatID$$aJCR$$bNAT IMMUNOL : 2021$$d2023-03-30
000153368 915__ $$0StatID:(DE-HGF)0200$$2StatID$$aDBCoverage$$bSCOPUS$$d2023-03-30
000153368 915__ $$0StatID:(DE-HGF)0300$$2StatID$$aDBCoverage$$bMedline$$d2023-03-30
000153368 915__ $$0StatID:(DE-HGF)0600$$2StatID$$aDBCoverage$$bEbsco Academic Search$$d2023-03-30
000153368 915__ $$0StatID:(DE-HGF)0030$$2StatID$$aPeer Review$$bASC$$d2023-03-30
000153368 915__ $$0StatID:(DE-HGF)0199$$2StatID$$aDBCoverage$$bClarivate Analytics Master Journal List$$d2023-03-30
000153368 915__ $$0StatID:(DE-HGF)0150$$2StatID$$aDBCoverage$$bWeb of Science Core Collection$$d2023-03-30
000153368 915__ $$0StatID:(DE-HGF)1050$$2StatID$$aDBCoverage$$bBIOSIS Previews$$d2023-03-30
000153368 915__ $$0StatID:(DE-HGF)1030$$2StatID$$aDBCoverage$$bCurrent Contents - Life Sciences$$d2023-03-30
000153368 915__ $$0StatID:(DE-HGF)9930$$2StatID$$aIF >= 30$$bNAT IMMUNOL : 2021$$d2023-03-30
000153368 9201_ $$0I:(DE-2719)5000007$$kAG Priller$$lTranslational Neuropsychiatry$$x0
000153368 980__ $$ajournal
000153368 980__ $$aVDB
000153368 980__ $$aI:(DE-2719)5000007
000153368 980__ $$aUNRESTRICTED