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@ARTICLE{Masuda:153368,
      author       = {Masuda, Takahiro and Amann, Lukas and Sankowski, Roman and
                      Staszewski, Ori and Lenz, Maximilian and D Errico, Paolo and
                      Snaidero, Nicolas and Costa Jordão, Marta Joana and
                      Böttcher, Chotima and Kierdorf, Katrin and Jung, Steffen
                      and Priller, Josef and Misgeld, Thomas and Vlachos, Andreas
                      and Meyer-Luehmann, Melanie and Knobeloch, Klaus-Peter and
                      Prinz, Marco},
      title        = {{N}ovel {H}exb-based tools for studying microglia in the
                      {CNS}.},
      journal      = {Nature immunology},
      volume       = {21},
      number       = {7},
      issn         = {1529-2916},
      address      = {London},
      publisher    = {Springer Nature Limited},
      reportid     = {DZNE-2020-01365},
      pages        = {802 - 815},
      year         = {2020},
      abstract     = {Microglia and central nervous system (CNS)-associated
                      macrophages (CAMs), such as perivascular and meningeal
                      macrophages, are implicated in virtually all diseases of the
                      CNS. However, little is known about their cell-type-specific
                      roles in the absence of suitable tools that would allow for
                      functional discrimination between the ontogenetically
                      closely related microglia and CAMs. To develop a new
                      microglia gene targeting model, we first applied massively
                      parallel single-cell analyses to compare microglia and CAM
                      signatures during homeostasis and disease and identified
                      hexosaminidase subunit beta (Hexb) as a stably expressed
                      microglia core gene, whereas other microglia core genes were
                      substantially downregulated during pathologies. Next, we
                      generated HexbtdTomato mice to stably monitor microglia
                      behavior in vivo. Finally, the Hexb locus was employed for
                      tamoxifen-inducible Cre-mediated gene manipulation in
                      microglia and for fate mapping of microglia but not CAMs. In
                      sum, we provide valuable new genetic tools to specifically
                      study microglia functions in the CNS.},
      keywords     = {Animals / Brain: cytology / Brain: immunology / Brain:
                      pathology / CRISPR-Cas Systems: genetics /
                      Encephalomyelitis, Autoimmune, Experimental: immunology /
                      Encephalomyelitis, Autoimmune, Experimental: pathology /
                      Facial Nerve Injuries: immunology / Facial Nerve Injuries:
                      pathology / Gene Knock-In Techniques / Genes, Reporter:
                      genetics / Genetic Loci: genetics / Humans / Intravital
                      Microscopy / Luminescent Agents: chemistry / Luminescent
                      Proteins: chemistry / Luminescent Proteins: genetics /
                      Macrophages: immunology / Macrophages: metabolism / Mice /
                      Microglia: immunology / Microglia: metabolism / NIH 3T3
                      Cells / RNA-Seq / Single-Cell Analysis / Transfection /
                      beta-Hexosaminidase beta Chain: genetics /
                      beta-Hexosaminidase beta Chain: metabolism / Luminescent
                      Agents (NLM Chemicals) / Luminescent Proteins (NLM
                      Chemicals) / red fluorescent protein (NLM Chemicals) /
                      beta-Hexosaminidase beta Chain (NLM Chemicals)},
      cin          = {AG Priller},
      ddc          = {610},
      cid          = {I:(DE-2719)5000007},
      pnm          = {344 - Clinical and Health Care Research (POF3-344)},
      pid          = {G:(DE-HGF)POF3-344},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:32541832},
      doi          = {10.1038/s41590-020-0707-4},
      url          = {https://pub.dzne.de/record/153368},
}